Efficient production of reuterin from glycerol by magnetically immobilized Lactobacillus reuteri
| LEADER | caa a22 4500 | ||
|---|---|---|---|
| 001 | 605506396 | ||
| 003 | CHVBK | ||
| 005 | 20210128100627.0 | ||
| 007 | cr unu---uuuuu | ||
| 008 | 210128e20150601xx s 000 0 eng | ||
| 024 | 7 | 0 | |a 10.1007/s00253-015-6530-4 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-015-6530-4 | ||
| 245 | 0 | 0 | |a Efficient production of reuterin from glycerol by magnetically immobilized Lactobacillus reuteri |h [Elektronische Daten] |c [Feixia Liu, Bo Yu] |
| 520 | 3 | |a Reuterin is a compound that contains the monomeric, hydrated monomeric, and cyclic dimeric forms of 3-hydroxypropionaldehyde (3-HPA). It is widely used as a food preservative due to its antimicrobial properties, and 3-HPA is also an important precursor for several industrial chemicals. In this study, we first developed an efficient immobilization process involving magnetic cell entrapment for production of reuterin from glycerol by Lactobacillus reuteri. The cell growth condition was optimized by statistical approaches. High conversion efficiency was achieved using k-carrageenan as the immobilization support and Fe3O4 as magnetic nanoparticles. Furthermore, addition of 0.02g/L vitamin B12, 10mmol/LMg2+, and glucose in a molar ratio of 0.15 to glycerol significantly increased the conversion rate. Under optimal conditions, 235.9mmol/L 3-HPA was produced from 250mmol/L initial glycerol in 1.5h, with a molar yield of 94.4% and a productivity of 15.4mmol/(g dry cell weight∙L∙h), which were the highest values to date. Thus, this study demonstrated a promising process for the improvement of biocatalyst efficiency in biotransformation. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a Reuterin |2 nationallicence | |
| 690 | 7 | |a Lactobacillus reuteri |2 nationallicence | |
| 690 | 7 | |a Magnetic immobilization |2 nationallicence | |
| 690 | 7 | |a Glycerol |2 nationallicence | |
| 690 | 7 | |a Dehydratase |2 nationallicence | |
| 700 | 1 | |a Liu |D Feixia |u CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, 100101, Beijing, People's Republic of China |4 aut | |
| 700 | 1 | |a Yu |D Bo |u CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, 100101, Beijing, People's Republic of China |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/11(2015-06-01), 4659-4666 |x 0175-7598 |q 99:11<4659 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6530-4 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6530-4 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Liu |D Feixia |u CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, 100101, Beijing, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Yu |D Bo |u CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, 100101, Beijing, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/11(2015-06-01), 4659-4666 |x 0175-7598 |q 99:11<4659 |1 2015 |2 99 |o 253 | ||