caa a22 4500 605506418 CHVBK 20210128100627.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s00253-014-6364-5 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6364-5 Structural diversity and possible functional roles of free fatty acids of the novel soil isolate Streptomyces sp. NP10 [Elektronische Daten] [Tatjana Ilic-Tomic, Marija Genčić, Milena Živković, Branka Vasiljevic, Lidija Djokic, Jasmina Nikodinovic-Runic, Niko Radulović] Herein, a novel soil bacterium Streptomyces sp. NP10 able to grow outside usual streptomycetes optimum conditions (e.g., at 4°C, pH 9 and high NaCl concentration), exhibiting atypical hemolytic, DNAse, and cellulolytic activities, is described. This strain produces and excretes into the growth medium large amounts of free long-chain fatty acids (FAs). A concurrent lipidomics study revealed a large structural diversity of FAs with over 50 different n- and branched-chain, (un)saturated, and cyclopropane FAs (C7-C30) produced by this strain. Two of these, i-17:0cy9-10 and a-18:0cy9-10, represent new natural products and the first ever identified branched cyclopropane FAs. Both free and bound lipid profiles of Streptomyces sp. NP10 were dominated by saturated branched chain FAs (i-14:0, a-15:0, and i-16:0). Although these free FAs showed only a moderate antimicrobial activity, our results suggest that they could have an ecophysiological role in interspecies signaling with another soil microorganism Pseudomonas aeruginosa. This work represents the first comprehensive report on the structural diversity and complexity of the free FA pool in Streptomyces. A naturally occurring streptomycete, such as Streptomyces sp. NP10, which secretes significant amounts of free long-chain FAs (non-cytotoxic) into the medium, could be useful in microbial biodiesel production. Springer-Verlag Berlin Heidelberg, 2015 Streptomyces sp. NP10 nationallicence Lipidomics nationallicence Free fatty acids nationallicence Branched cyclopropane fatty acids nationallicence Ecophysiological role nationallicence Microbial biodiesel production nationallicence Ilic-Tomic Tatjana Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, P.O. Box 23, 11010, Belgrade, Serbia aut Genčić Marija Department of Chemistry, Faculty of Science and Mathematics, University of Niš, Višegradska 33, 18000, Niš, Serbia aut Živković Milena Department of Chemistry, Faculty of Science and Mathematics, University of Niš, Višegradska 33, 18000, Niš, Serbia aut Vasiljevic Branka Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, P.O. Box 23, 11010, Belgrade, Serbia aut Djokic Lidija Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, P.O. Box 23, 11010, Belgrade, Serbia aut Nikodinovic-Runic Jasmina Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, P.O. Box 23, 11010, Belgrade, Serbia aut Radulović Niko Department of Chemistry, Faculty of Science and Mathematics, University of Niš, Višegradska 33, 18000, Niš, Serbia aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/11(2015-06-01), 4815-4833 0175-7598 99:11<4815 2015 99 253 https://doi.org/10.1007/s00253-014-6364-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6364-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ilic-Tomic Tatjana Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, P.O. Box 23, 11010, Belgrade, Serbia aut NATIONALLICENCE 700 1- Genčić Marija Department of Chemistry, Faculty of Science and Mathematics, University of Niš, Višegradska 33, 18000, Niš, Serbia aut NATIONALLICENCE 700 1- Živković Milena Department of Chemistry, Faculty of Science and Mathematics, University of Niš, Višegradska 33, 18000, Niš, Serbia aut NATIONALLICENCE 700 1- Vasiljevic Branka Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, P.O. Box 23, 11010, Belgrade, Serbia aut NATIONALLICENCE 700 1- Djokic Lidija Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, P.O. Box 23, 11010, Belgrade, Serbia aut NATIONALLICENCE 700 1- Nikodinovic-Runic Jasmina Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, P.O. Box 23, 11010, Belgrade, Serbia aut NATIONALLICENCE 700 1- Radulović Niko Department of Chemistry, Faculty of Science and Mathematics, University of Niš, Višegradska 33, 18000, Niš, Serbia aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/11(2015-06-01), 4815-4833 0175-7598 99:11<4815 2015 99 253