Potent antimicrobial peptides against Legionella pneumophila and its environmental host, Acanthamoeba castellanii
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| 024 | 7 | 0 | |a 10.1007/s00253-015-6381-z |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-015-6381-z | ||
| 245 | 0 | 0 | |a Potent antimicrobial peptides against Legionella pneumophila and its environmental host, Acanthamoeba castellanii |h [Elektronische Daten] |c [Margot Schlusselhuber, Vincent Humblot, Sandra Casale, Christophe Méthivier, Julien Verdon, Matthias Leippe, Jean-Marc Berjeaud] |
| 520 | 3 | |a Legionella pneumophila, the major causative agent of Legionnaires' disease, is most often found in the environment in close association with free-living amoebae, leading to persistence, spread, biocide resistance, and elevated virulence of the bacterium. In the present study, we evaluated the anti-Legionella and anti-Acanthamoeba activities of three alpha-helical antimicrobial peptides (AMPs), namely, NK-2, Ci-MAM-A24, and Ci-PAP-A22, already known for the extraordinary efficacy against other microbes. Our data represent the first demonstration of the activity of a particular AMP against both the human facultative intracellular pathogen L. pneumophila and its pathogenic host, Acanthamoeba castellanii. Interestingly, the most effective peptide, Ci-MAM-A24, was also found to reduce the Legionella cell number within amoebae. Accordingly, this peptide was immobilized on gold surfaces to assess its antimicrobial activity. Surfaces were characterized, and activity studies revealed that the potent bactericidal activity of the peptide was conserved after its immobilization. In the frame of elaborating anti-Legionella surfaces, Ci-MAM-A24 represents, by its direct and indirect activity against Legionella, a potent peptide template for biological control of the bacterium in plumbings. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a Legionella |2 nationallicence | |
| 690 | 7 | |a Antimicrobial peptides |2 nationallicence | |
| 690 | 7 | |a Amoebae |2 nationallicence | |
| 690 | 7 | |a Surface modification |2 nationallicence | |
| 700 | 1 | |a Schlusselhuber |D Margot |u Laboratoire Ecologie & Biologie des Interactions, UMR CNRS 7267, Equipe Microbiologie de l'Eau, Université de Poitiers, 1 Rue Georges Bonnet, TSA 51106, 86073, Poitiers Cedex 9, France |4 aut | |
| 700 | 1 | |a Humblot |D Vincent |u UPMC Univ Paris 06, UMR 7197, Laboratoire de Réactivité de Surface (LRS), Sorbonne Universités, 75005, Paris, France |4 aut | |
| 700 | 1 | |a Casale |D Sandra |u UPMC Univ Paris 06, UMR 7197, Laboratoire de Réactivité de Surface (LRS), Sorbonne Universités, 75005, Paris, France |4 aut | |
| 700 | 1 | |a Méthivier |D Christophe |u UPMC Univ Paris 06, UMR 7197, Laboratoire de Réactivité de Surface (LRS), Sorbonne Universités, 75005, Paris, France |4 aut | |
| 700 | 1 | |a Verdon |D Julien |u Laboratoire Ecologie & Biologie des Interactions, UMR CNRS 7267, Equipe Microbiologie de l'Eau, Université de Poitiers, 1 Rue Georges Bonnet, TSA 51106, 86073, Poitiers Cedex 9, France |4 aut | |
| 700 | 1 | |a Leippe |D Matthias |u Zoological Institute, Comparative Immunobiology, Christian-Albrechts University of Kiel, Olshausenstrasse 40, 24098, Kiel, Germany |4 aut | |
| 700 | 1 | |a Berjeaud |D Jean-Marc |u Laboratoire Ecologie & Biologie des Interactions, UMR CNRS 7267, Equipe Microbiologie de l'Eau, Université de Poitiers, 1 Rue Georges Bonnet, TSA 51106, 86073, Poitiers Cedex 9, France |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/11(2015-06-01), 4879-4891 |x 0175-7598 |q 99:11<4879 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6381-z |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6381-z |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Schlusselhuber |D Margot |u Laboratoire Ecologie & Biologie des Interactions, UMR CNRS 7267, Equipe Microbiologie de l'Eau, Université de Poitiers, 1 Rue Georges Bonnet, TSA 51106, 86073, Poitiers Cedex 9, France |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Humblot |D Vincent |u UPMC Univ Paris 06, UMR 7197, Laboratoire de Réactivité de Surface (LRS), Sorbonne Universités, 75005, Paris, France |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Casale |D Sandra |u UPMC Univ Paris 06, UMR 7197, Laboratoire de Réactivité de Surface (LRS), Sorbonne Universités, 75005, Paris, France |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Méthivier |D Christophe |u UPMC Univ Paris 06, UMR 7197, Laboratoire de Réactivité de Surface (LRS), Sorbonne Universités, 75005, Paris, France |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Verdon |D Julien |u Laboratoire Ecologie & Biologie des Interactions, UMR CNRS 7267, Equipe Microbiologie de l'Eau, Université de Poitiers, 1 Rue Georges Bonnet, TSA 51106, 86073, Poitiers Cedex 9, France |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Leippe |D Matthias |u Zoological Institute, Comparative Immunobiology, Christian-Albrechts University of Kiel, Olshausenstrasse 40, 24098, Kiel, Germany |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Berjeaud |D Jean-Marc |u Laboratoire Ecologie & Biologie des Interactions, UMR CNRS 7267, Equipe Microbiologie de l'Eau, Université de Poitiers, 1 Rue Georges Bonnet, TSA 51106, 86073, Poitiers Cedex 9, France |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/11(2015-06-01), 4879-4891 |x 0175-7598 |q 99:11<4879 |1 2015 |2 99 |o 253 | ||