caa a22 4500 605506442 CHVBK 20210128100627.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s00253-015-6381-z doi (NATIONALLICENCE)springer-10.1007/s00253-015-6381-z Potent antimicrobial peptides against Legionella pneumophila and its environmental host, Acanthamoeba castellanii [Elektronische Daten] [Margot Schlusselhuber, Vincent Humblot, Sandra Casale, Christophe Méthivier, Julien Verdon, Matthias Leippe, Jean-Marc Berjeaud] Legionella pneumophila, the major causative agent of Legionnaires' disease, is most often found in the environment in close association with free-living amoebae, leading to persistence, spread, biocide resistance, and elevated virulence of the bacterium. In the present study, we evaluated the anti-Legionella and anti-Acanthamoeba activities of three alpha-helical antimicrobial peptides (AMPs), namely, NK-2, Ci-MAM-A24, and Ci-PAP-A22, already known for the extraordinary efficacy against other microbes. Our data represent the first demonstration of the activity of a particular AMP against both the human facultative intracellular pathogen L. pneumophila and its pathogenic host, Acanthamoeba castellanii. Interestingly, the most effective peptide, Ci-MAM-A24, was also found to reduce the Legionella cell number within amoebae. Accordingly, this peptide was immobilized on gold surfaces to assess its antimicrobial activity. Surfaces were characterized, and activity studies revealed that the potent bactericidal activity of the peptide was conserved after its immobilization. In the frame of elaborating anti-Legionella surfaces, Ci-MAM-A24 represents, by its direct and indirect activity against Legionella, a potent peptide template for biological control of the bacterium in plumbings. Springer-Verlag Berlin Heidelberg, 2015 Legionella nationallicence Antimicrobial peptides nationallicence Amoebae nationallicence Surface modification nationallicence Schlusselhuber Margot Laboratoire Ecologie & Biologie des Interactions, UMR CNRS 7267, Equipe Microbiologie de l'Eau, Université de Poitiers, 1 Rue Georges Bonnet, TSA 51106, 86073, Poitiers Cedex 9, France aut Humblot Vincent UPMC Univ Paris 06, UMR 7197, Laboratoire de Réactivité de Surface (LRS), Sorbonne Universités, 75005, Paris, France aut Casale Sandra UPMC Univ Paris 06, UMR 7197, Laboratoire de Réactivité de Surface (LRS), Sorbonne Universités, 75005, Paris, France aut Méthivier Christophe UPMC Univ Paris 06, UMR 7197, Laboratoire de Réactivité de Surface (LRS), Sorbonne Universités, 75005, Paris, France aut Verdon Julien Laboratoire Ecologie & Biologie des Interactions, UMR CNRS 7267, Equipe Microbiologie de l'Eau, Université de Poitiers, 1 Rue Georges Bonnet, TSA 51106, 86073, Poitiers Cedex 9, France aut Leippe Matthias Zoological Institute, Comparative Immunobiology, Christian-Albrechts University of Kiel, Olshausenstrasse 40, 24098, Kiel, Germany aut Berjeaud Jean-Marc Laboratoire Ecologie & Biologie des Interactions, UMR CNRS 7267, Equipe Microbiologie de l'Eau, Université de Poitiers, 1 Rue Georges Bonnet, TSA 51106, 86073, Poitiers Cedex 9, France aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/11(2015-06-01), 4879-4891 0175-7598 99:11<4879 2015 99 253 https://doi.org/10.1007/s00253-015-6381-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6381-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Schlusselhuber Margot Laboratoire Ecologie & Biologie des Interactions, UMR CNRS 7267, Equipe Microbiologie de l'Eau, Université de Poitiers, 1 Rue Georges Bonnet, TSA 51106, 86073, Poitiers Cedex 9, France aut NATIONALLICENCE 700 1- Humblot Vincent UPMC Univ Paris 06, UMR 7197, Laboratoire de Réactivité de Surface (LRS), Sorbonne Universités, 75005, Paris, France aut NATIONALLICENCE 700 1- Casale Sandra UPMC Univ Paris 06, UMR 7197, Laboratoire de Réactivité de Surface (LRS), Sorbonne Universités, 75005, Paris, France aut NATIONALLICENCE 700 1- Méthivier Christophe UPMC Univ Paris 06, UMR 7197, Laboratoire de Réactivité de Surface (LRS), Sorbonne Universités, 75005, Paris, France aut NATIONALLICENCE 700 1- Verdon Julien Laboratoire Ecologie & Biologie des Interactions, UMR CNRS 7267, Equipe Microbiologie de l'Eau, Université de Poitiers, 1 Rue Georges Bonnet, TSA 51106, 86073, Poitiers Cedex 9, France aut NATIONALLICENCE 700 1- Leippe Matthias Zoological Institute, Comparative Immunobiology, Christian-Albrechts University of Kiel, Olshausenstrasse 40, 24098, Kiel, Germany aut NATIONALLICENCE 700 1- Berjeaud Jean-Marc Laboratoire Ecologie & Biologie des Interactions, UMR CNRS 7267, Equipe Microbiologie de l'Eau, Université de Poitiers, 1 Rue Georges Bonnet, TSA 51106, 86073, Poitiers Cedex 9, France aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/11(2015-06-01), 4879-4891 0175-7598 99:11<4879 2015 99 253