caa a22 4500 605506493 CHVBK 20210128100627.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s00253-015-6587-0 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6587-0 cAMP receptor protein (CRP)-mediated resistance/tolerance in bacteria: mechanism and utilization in biotechnology [Elektronische Daten] [Hefang Geng, Rongrong Jiang] Cyclic AMP receptor protein (CRP) is one of the seven global regulators in Escherichia coli, which regulates the expression of over 490 genes. It contains a cAMP binding N-terminal domain and a DNA binding C-terminal domain, connected via a short hinge region. Various stress-tolerant E. coli mutants had been obtained through transcriptional engineering of CRP. This review aims to shed some light on the possible mechanism behind these CRP variants, from the change in CRP structure, transcription profile, and DNA binding affinity. The amino acid substitutions are distributed along the protein—certain mutations have shown higher frequency than others, such as T127N and D138Y. β-Galactosidase reporter gene assay revealed that CRP mutants had lower binding affinity with all three classes of CRP-dependent promoters as compared to native CRP, which probably would change cellular transcription profile. Different CRP mutants would induce different cellular transcription profile in E. coli, but there are common genes differentially expressed in these variants, including upregulated gadAB and downregulated nontransporter genes aspA and tnaA, and transporter/poringenes malE, mglB, cstA, and lamB. We believe that transcriptional engineering of CRP can provide an alternative strain engineering method for E. coli and its detailed mechanism may need further investigations. Springer-Verlag Berlin Heidelberg, 2015 Global transcription factor nationallicence cAMP receptor protein nationallicence Strain engineering nationallicence Stress tolerance nationallicence Random mutagenesis nationallicence Directed evolution nationallicence Transcriptional engineering nationallicence DNA microarray nationallicence CRP structure nationallicence Global transcription machinery engineering nationallicence Geng Hefang School of Chemical and Biomedical Engineering, Nanyang Technological University, 62 Nanyang Drive, 637459, Singapore, Singapore aut Jiang Rongrong School of Chemical and Biomedical Engineering, Nanyang Technological University, 62 Nanyang Drive, 637459, Singapore, Singapore aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/11(2015-06-01), 4533-4543 0175-7598 99:11<4533 2015 99 253 https://doi.org/10.1007/s00253-015-6587-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 review-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6587-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Geng Hefang School of Chemical and Biomedical Engineering, Nanyang Technological University, 62 Nanyang Drive, 637459, Singapore, Singapore aut NATIONALLICENCE 700 1- Jiang Rongrong School of Chemical and Biomedical Engineering, Nanyang Technological University, 62 Nanyang Drive, 637459, Singapore, Singapore aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/11(2015-06-01), 4533-4543 0175-7598 99:11<4533 2015 99 253