caa a22 4500 605506515 CHVBK 20210128100627.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s00253-015-6608-z doi (NATIONALLICENCE)springer-10.1007/s00253-015-6608-z A fungal biofilm reactor based on metal structured packing improves the quality of a Gla::GFP fusion protein produced by Aspergillus oryzae [Elektronische Daten] [Q. Zune, A. Delepierre, S. Gofflot, J. Bauwens, J. Twizere, P. Punt, F. Francis, D. Toye, T. Bawin, F. Delvigne] Fungal biofilm is known to promote the excretion of secondary metabolites in accordance with solid-state-related physiological mechanisms. This work is based on the comparative analysis of classical submerged fermentation with a fungal biofilm reactor for the production of a Gla::green fluorescent protein (GFP) fusion protein by Aspergillus oryzae. The biofilm reactor comprises a metal structured packing allowing the attachment of the fungal biomass. Since the production of the target protein is under the control of the promoter glaB, specifically induced in solid-state fermentation, the biofilm mode of culture is expected to enhance the global productivity. Although production of the target protein was enhanced by using the biofilm mode of culture, we also found that fusion protein production is also significant when the submerged mode of culture is used. This result is related to high shear stress leading to biomass autolysis and leakage of intracellular fusion protein into the extracellular medium. Moreover, 2-D gel electrophoresis highlights the preservation of fusion protein integrity produced in biofilm conditions. Two fungal biofilm reactor designs were then investigated further, i.e. with full immersion of the packing or with medium recirculation on the packing, and the scale-up potentialities were evaluated. In this context, it has been shown that full immersion of the metal packing in the liquid medium during cultivation allows for a uniform colonization of the packing by the fungal biomass and leads to a better quality of the fusion protein. Springer-Verlag Berlin Heidelberg, 2015 Fungal biofilm nationallicence Bioreactor nationallicence Scale-up nationallicence Recombinant protein nationallicence Zune Q. Microbial Processes and Interactions (MiPI), Gembloux ABT (ULg), 2 Passage des Déportés, 5030, Gembloux, Belgium aut Delepierre A. Microbial Processes and Interactions (MiPI), Gembloux ABT (ULg), 2 Passage des Déportés, 5030, Gembloux, Belgium aut Gofflot S. Unit of Products Transformation Technology, CRAW, 24 Chaussée de Namur, 5030, Gembloux, Belgium aut Bauwens J. Unit of Functional and Evolutionary Entomology, Gembloux ABT (ULg), 2 Passage des Déportés, 5030, Gembloux, Belgium aut Twizere J. Unit of Cellular and Molecular Biology, Gembloux ABT (ULg), 2 Passage des Déportés, 5030, Gembloux, Belgium aut Punt P. TNO Microbiology & Systems Biology, 3600 AJ, Zeist, The Netherlands aut Francis F. Unit of Functional and Evolutionary Entomology, Gembloux ABT (ULg), 2 Passage des Déportés, 5030, Gembloux, Belgium aut Toye D. Laboratory of Chemical Engineering, ULg, Bâtiment B6c, Sart Tilman, 4000, Liège, Belgium aut Bawin T. Unit of Functional and Evolutionary Entomology, Gembloux ABT (ULg), 2 Passage des Déportés, 5030, Gembloux, Belgium aut Delvigne F. Microbial Processes and Interactions (MiPI), Gembloux ABT (ULg), 2 Passage des Déportés, 5030, Gembloux, Belgium aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/15(2015-08-01), 6241-6254 0175-7598 99:15<6241 2015 99 253 https://doi.org/10.1007/s00253-015-6608-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6608-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zune Q. Microbial Processes and Interactions (MiPI), Gembloux ABT (ULg), 2 Passage des Déportés, 5030, Gembloux, Belgium aut NATIONALLICENCE 700 1- Delepierre A. Microbial Processes and Interactions (MiPI), Gembloux ABT (ULg), 2 Passage des Déportés, 5030, Gembloux, Belgium aut NATIONALLICENCE 700 1- Gofflot S. Unit of Products Transformation Technology, CRAW, 24 Chaussée de Namur, 5030, Gembloux, Belgium aut NATIONALLICENCE 700 1- Bauwens J. Unit of Functional and Evolutionary Entomology, Gembloux ABT (ULg), 2 Passage des Déportés, 5030, Gembloux, Belgium aut NATIONALLICENCE 700 1- Twizere J. Unit of Cellular and Molecular Biology, Gembloux ABT (ULg), 2 Passage des Déportés, 5030, Gembloux, Belgium aut NATIONALLICENCE 700 1- Punt P. TNO Microbiology & Systems Biology, 3600 AJ, Zeist, The Netherlands aut NATIONALLICENCE 700 1- Francis F. Unit of Functional and Evolutionary Entomology, Gembloux ABT (ULg), 2 Passage des Déportés, 5030, Gembloux, Belgium aut NATIONALLICENCE 700 1- Toye D. Laboratory of Chemical Engineering, ULg, Bâtiment B6c, Sart Tilman, 4000, Liège, Belgium aut NATIONALLICENCE 700 1- Bawin T. Unit of Functional and Evolutionary Entomology, Gembloux ABT (ULg), 2 Passage des Déportés, 5030, Gembloux, Belgium aut NATIONALLICENCE 700 1- Delvigne F. Microbial Processes and Interactions (MiPI), Gembloux ABT (ULg), 2 Passage des Déportés, 5030, Gembloux, Belgium aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/15(2015-08-01), 6241-6254 0175-7598 99:15<6241 2015 99 253