Physiologo-biochemical characteristics of citrate-producing yeast Yarrowia lipolytica grown on glycerol-containing waste of biodiesel industry

Verfasser / Beitragende:
[Igor Morgunov, Svetlana Kamzolova]
Ort, Verlag, Jahr:
2015
Enthalten in:
Applied Microbiology and Biotechnology, 99/15(2015-08-01), 6443-6450
Format:
Artikel (online)
ID: 605506531
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024 7 0 |a 10.1007/s00253-015-6558-5  |2 doi 
035 |a (NATIONALLICENCE)springer-10.1007/s00253-015-6558-5 
245 0 0 |a Physiologo-biochemical characteristics of citrate-producing yeast Yarrowia lipolytica grown on glycerol-containing waste of biodiesel industry  |h [Elektronische Daten]  |c [Igor Morgunov, Svetlana Kamzolova] 
520 3 |a In this study, physiologo-biochemical characteristics of citrate-producing yeast Yarrowia lipolytica grown on glycerol-containing waste of biodiesel industry were studied by an investigation of growth dynamics, the consumption of glycerol, and the fatty acid fractions from waste as well as by measuring the activities of enzymes involved in the metabolism of waste. It was shown that Y. lipolytica realizes concurrent uptake of glycerol and the fatty acid fractions during conversion of glycerol-containing waste, although glycerol was utilized at a higher rate than fatty acids. Under optimal feeding of glycerol-containing waste by portions of 20gl−1, the citric acid production and the ratio between citric acid and isocitric acid depended on the strain used. It was revealed that wild strain Y. lipolytica VKM Y-2373 produced citrate and isocitrate with a ratio of 1.7:1, while the mutant strain Y. lipolytica NG40/UV7 synthesized presumably citric acid (122.2gl−1) with a citrate-to-isocitrate ratio of 53:1 and the yield of 0.95gg−1. 
540 |a Springer-Verlag Berlin Heidelberg, 2015 
690 7 |a Glycerol-containing waste (GW)  |2 nationallicence 
690 7 |a Biodiesel industry  |2 nationallicence 
690 7 |a Citric acid (CA) production  |2 nationallicence 
690 7 |a Isocitric acid (ICA) production  |2 nationallicence 
690 7 |a Yarrowia lipolytica  |2 nationallicence 
690 7 |a Glycerol  |2 nationallicence 
690 7 |a Fatty acids  |2 nationallicence 
700 1 |a Morgunov  |D Igor  |u G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, 142290, Moscow, Russia  |4 aut 
700 1 |a Kamzolova  |D Svetlana  |u G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, 142290, Moscow, Russia  |4 aut 
773 0 |t Applied Microbiology and Biotechnology  |d Springer Berlin Heidelberg  |g 99/15(2015-08-01), 6443-6450  |x 0175-7598  |q 99:15<6443  |1 2015  |2 99  |o 253 
856 4 0 |u https://doi.org/10.1007/s00253-015-6558-5  |q text/html  |z Onlinezugriff via DOI 
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900 7 |a Metadata rights reserved  |b Springer special CC-BY-NC licence  |2 nationallicence 
908 |D 1  |a research-article  |2 jats 
949 |B NATIONALLICENCE  |F NATIONALLICENCE  |b NL-springer 
950 |B NATIONALLICENCE  |P 856  |E 40  |u https://doi.org/10.1007/s00253-015-6558-5  |q text/html  |z Onlinezugriff via DOI 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Morgunov  |D Igor  |u G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, 142290, Moscow, Russia  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Kamzolova  |D Svetlana  |u G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, 142290, Moscow, Russia  |4 aut 
950 |B NATIONALLICENCE  |P 773  |E 0-  |t Applied Microbiology and Biotechnology  |d Springer Berlin Heidelberg  |g 99/15(2015-08-01), 6443-6450  |x 0175-7598  |q 99:15<6443  |1 2015  |2 99  |o 253