caa a22 4500 60550654X CHVBK 20210128100627.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s00253-015-6555-8 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6555-8 Outer membrane proteins related to SusC and SusD are not required for Cytophaga hutchinsonii cellulose utilization [Elektronische Daten] [Yongtao Zhu, Kurt Kwiatkowski, Tengteng Yang, Sampada Kharade, Constance Bahr, Nicole Koropatkin, Weifeng Liu, Mark McBride] Cytophaga hutchinsonii, a member of the phylum Bacteroidetes, employs a novel collection of cell-associated proteins to digest crystalline cellulose. Other Bacteroidetes rely on cell surface proteins related to the starch utilization system (Sus) proteins SusC and SusD to bind oligosaccharides and import them across the outer membrane for further digestion. These bacteria typically produce dozens of SusC-like porins and SusD-like oligosaccharide-binding proteins to facilitate utilization of diverse polysaccharides. C. hutchinsonii specializes in cellulose digestion and its genome has only two susC-like genes and two susD-like genes. Single and multiple gene deletions were constructed to determine if the susC-like and susD-like genes have roles in cellulose utilization. A mutant lacking all susC-like and all susD-like genes digested cellulose and grew on cellulose as well as wild-type cells. Further, recombinantly expressed SusD-like proteins CHU_0547 and CHU_0554 failed to bind cellulose or β-glucan hemicellulosic polysaccharides. The results suggest that the Bacteroidetes Sus paradigm for polysaccharide utilization may not apply to the cellulolytic bacterium C. hutchinsonii. Springer-Verlag Berlin Heidelberg, 2015 Cellulose nationallicence Bacteroidetes nationallicence Polysaccharide utilization nationallicence SusD nationallicence Zhu Yongtao Department of Biological Sciences, University of Wisconsin-Milwaukee, P.O. Box 413, 53201, Milwaukee, WI, USA aut Kwiatkowski Kurt Department of Microbiology and Immunology, University of Michigan Medical School, Medical Sciences II, Room 5641, 48109, Ann Arbor, MI, USA aut Yang Tengteng The State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, 250100, Jinan, Shandong, China aut Kharade Sampada Department of Biological Sciences, University of Wisconsin-Milwaukee, P.O. Box 413, 53201, Milwaukee, WI, USA aut Bahr Constance Department of Microbiology and Immunology, University of Michigan Medical School, Medical Sciences II, Room 5641, 48109, Ann Arbor, MI, USA aut Koropatkin Nicole Department of Microbiology and Immunology, University of Michigan Medical School, Medical Sciences II, Room 5641, 48109, Ann Arbor, MI, USA aut Liu Weifeng The State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, 250100, Jinan, Shandong, China aut McBride Mark Department of Biological Sciences, University of Wisconsin-Milwaukee, P.O. Box 413, 53201, Milwaukee, WI, USA aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/15(2015-08-01), 6339-6350 0175-7598 99:15<6339 2015 99 253 https://doi.org/10.1007/s00253-015-6555-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6555-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhu Yongtao Department of Biological Sciences, University of Wisconsin-Milwaukee, P.O. Box 413, 53201, Milwaukee, WI, USA aut NATIONALLICENCE 700 1- Kwiatkowski Kurt Department of Microbiology and Immunology, University of Michigan Medical School, Medical Sciences II, Room 5641, 48109, Ann Arbor, MI, USA aut NATIONALLICENCE 700 1- Yang Tengteng The State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, 250100, Jinan, Shandong, China aut NATIONALLICENCE 700 1- Kharade Sampada Department of Biological Sciences, University of Wisconsin-Milwaukee, P.O. Box 413, 53201, Milwaukee, WI, USA aut NATIONALLICENCE 700 1- Bahr Constance Department of Microbiology and Immunology, University of Michigan Medical School, Medical Sciences II, Room 5641, 48109, Ann Arbor, MI, USA aut NATIONALLICENCE 700 1- Koropatkin Nicole Department of Microbiology and Immunology, University of Michigan Medical School, Medical Sciences II, Room 5641, 48109, Ann Arbor, MI, USA aut NATIONALLICENCE 700 1- Liu Weifeng The State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, 250100, Jinan, Shandong, China aut NATIONALLICENCE 700 1- McBride Mark Department of Biological Sciences, University of Wisconsin-Milwaukee, P.O. Box 413, 53201, Milwaukee, WI, USA aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/15(2015-08-01), 6339-6350 0175-7598 99:15<6339 2015 99 253