caa a22 4500 605506566 CHVBK 20210128100627.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s00253-015-6526-0 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6526-0 Effect of pH and dilution rate on specific production rate of extra cellular metabolites by Lactobacillus salivarius UCO_979C in continuous culture [Elektronische Daten] [Javier Valenzuela, Luis Pinuer, Apolinaria Cancino, Rodrigo Yáñez] The effect of pH and dilution rate on the production of extracellular metabolites of Lactobacillus salivarius UCO_979 was studied. The experiments were carried out in continuous mode, with chemically defined culture medium at a temperature of 37°C, 200rpm agitation and synthetic air flow of 100ml/min. Ethanol, acetic acid, formic acid, lactic acid and glucose were quantified through HPLC, while exopolysaccharide (EPS) was extracted with ethanol and quantified through the Dubois method. The results showed no linear trends for the specific production of lactic acid, EPS, acetic acid and ethanol, while the specific glucose consumption and ATP production rates showed linear trends. There was a metabolic change of the strain for dilution rates below 0.3h−1. The pH had a significant effect on the metabolism of the strain, which was evidenced by a higher specific glucose consumption and increased production of ATP at pH6 compared with that obtained at pH7. This work shows not only the metabolic capabilities of L. salivarius UCO_979C, but also shows that it is possible to quantify some molecules associated with its current use as gastrointestinal probiotic, especially regarding the production of organic acids and EPS. Springer-Verlag Berlin Heidelberg, 2015 Lactobacillus salivarius nationallicence pH and dilution rate nationallicence Continuous cultures nationallicence Metabolites nationallicence Valenzuela Javier Department of Chemical Engineering, Universidad de Concepción, Campus Concepción, Concepción, Chile aut Pinuer Luis Department of Microbiology, Universidad de Concepción, Campus Concepción, Concepción, Chile aut Cancino Apolinaria Department of Microbiology, Universidad de Concepción, Campus Concepción, Concepción, Chile aut Yáñez Rodrigo Department of Chemical Engineering, Universidad de Concepción, Campus Concepción, Concepción, Chile aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/15(2015-08-01), 6417-6429 0175-7598 99:15<6417 2015 99 253 https://doi.org/10.1007/s00253-015-6526-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6526-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Valenzuela Javier Department of Chemical Engineering, Universidad de Concepción, Campus Concepción, Concepción, Chile aut NATIONALLICENCE 700 1- Pinuer Luis Department of Microbiology, Universidad de Concepción, Campus Concepción, Concepción, Chile aut NATIONALLICENCE 700 1- Cancino Apolinaria Department of Microbiology, Universidad de Concepción, Campus Concepción, Concepción, Chile aut NATIONALLICENCE 700 1- Yáñez Rodrigo Department of Chemical Engineering, Universidad de Concepción, Campus Concepción, Concepción, Chile aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/15(2015-08-01), 6417-6429 0175-7598 99:15<6417 2015 99 253