caa a22 4500 605506574 CHVBK 20210128100627.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s00253-015-6706-y doi (NATIONALLICENCE)springer-10.1007/s00253-015-6706-y Transcriptome analysis of acetic-acid-treated yeast cells identifies a large set of genes whose overexpression or deletion enhances acetic acid tolerance [Elektronische Daten] [Yeji Lee, Olviyani Nasution, Eunyong Choi, In-Geol Choi, Wankee Kim, Wonja Choi] Acetic acid inhibits the metabolic activities of Saccharomyces cerevisiae. Therefore, a better understanding of how S. cerevisiae cells acquire the tolerance to acetic acid is of importance to develop robust yeast strains to be used in industry. To do this, we examined the transcriptional changes that occur at 12h post-exposure to acetic acid, revealing that 56 and 58 genes were upregulated and downregulated, respectively. Functional categorization of them revealed that 22 protein synthesis genes and 14 stress response genes constituted the largest portion of the upregulated and downregulated genes, respectively. To evaluate the association of the regulated genes with acetic acid tolerance, 3 upregulated genes (DBP2, ASC1, and GND1) were selected among 34 non-protein synthesis genes, and 54 viable mutants individually deleted for the downregulated genes were retrieved from the non-essential haploid deletion library. Strains overexpressing ASC1 and GND1 displayed enhanced tolerance to acetic acid, whereas a strain overexpressing DBP2 was sensitive. Fifty of 54 deletion mutants displayed enhanced acetic acid tolerance. Three chosen deletion mutants (hsps82Δ, ato2Δ, and ssa3Δ) were also tolerant to benzoic acid but not propionic and sorbic acids. Moreover, all those five (two overexpressing and three deleted) strains were more efficient in proton efflux and lower in membrane permeability and internal hydrogen peroxide content than controls. Individually or in combination, those physiological changes are likely to contribute at least in part to enhanced acetic acid tolerance. Overall, information of our transcriptional profile was very useful to identify molecular factors associated with acetic acid tolerance. Springer-Verlag Berlin Heidelberg, 2015 Transcriptome profile nationallicence Acetic acid nationallicence Overexpression nationallicence Deletion mutant nationallicence Stress tolerance nationallicence Lee Yeji Division of Life and Pharmaceutical Sciences, Ewha Womans University, 120-750, Seoul, Republic of Korea aut Nasution Olviyani Division of Life and Pharmaceutical Sciences, Ewha Womans University, 120-750, Seoul, Republic of Korea aut Choi Eunyong Division of Life and Pharmaceutical Sciences, Ewha Womans University, 120-750, Seoul, Republic of Korea aut Choi In-Geol School of Life Sciences and Biotechnology, Korea University, 136-713, Seoul, Republic of Korea aut Kim Wankee Department of Pharmacology, School of Medicine, Ajou University, 442-749, Suwon, Republic of Korea aut Choi Wonja Division of Life and Pharmaceutical Sciences, Ewha Womans University, 120-750, Seoul, Republic of Korea aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/15(2015-08-01), 6391-6403 0175-7598 99:15<6391 2015 99 253 https://doi.org/10.1007/s00253-015-6706-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6706-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lee Yeji Division of Life and Pharmaceutical Sciences, Ewha Womans University, 120-750, Seoul, Republic of Korea aut NATIONALLICENCE 700 1- Nasution Olviyani Division of Life and Pharmaceutical Sciences, Ewha Womans University, 120-750, Seoul, Republic of Korea aut NATIONALLICENCE 700 1- Choi Eunyong Division of Life and Pharmaceutical Sciences, Ewha Womans University, 120-750, Seoul, Republic of Korea aut NATIONALLICENCE 700 1- Choi In-Geol School of Life Sciences and Biotechnology, Korea University, 136-713, Seoul, Republic of Korea aut NATIONALLICENCE 700 1- Kim Wankee Department of Pharmacology, School of Medicine, Ajou University, 442-749, Suwon, Republic of Korea aut NATIONALLICENCE 700 1- Choi Wonja Division of Life and Pharmaceutical Sciences, Ewha Womans University, 120-750, Seoul, Republic of Korea aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/15(2015-08-01), 6391-6403 0175-7598 99:15<6391 2015 99 253