caa a22 4500 605506590 CHVBK 20210128100628.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s00253-015-6532-2 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6532-2 Evaluation of simultaneous autotrophic and heterotrophic denitrification processes and bacterial community structure analysis [Elektronische Daten] [Guihua Xu, Jingjing Peng, Cuijie Feng, Fang Fang, Shaohua Chen, Yuanjian Xu, Xingzu Wang] This study demonstrated that a combined heterotrophic and autotrophic denitrification (HAD) process is highly effective for the simultaneous removal of acetate, nitrate, and sulfide at an efficiency of 100, 80, and 100%, respectively. In the HAD system, simultaneous sulfide, acetate, and nitrate removals were observed, which indicated that heterotrophic and autotrophic denitrification occurred simultaneously. When the sulfide was existed in HAD reactor, the main product of sulfide biooxidation was S0. Once the sulfide was exhausted, the sulfate concentration in the HAD reactor increased and became the main end product. These results provided an alternative method to control the end sulfide biooxidation product by online monitoring sulfide concentration. Nearly half (43%) of the total clones in our mix-trophic reactor were amphitrophy denitrifiers. The autotrophic denitrifiers, heterotrophic denitrifiers, and amphitrophy denitrifiers coexisted in the HAD reactor to complete the denitrification process. Retrieved bacterial 16S rRNA gene clones affiliated with uncultured Xanthomonadaceae, Thauera, Thiobacillus, and Chromatiales were dominant. Springer-Verlag Berlin Heidelberg, 2015 Sulfide biooxidation nationallicence Autotrophic denitrification nationallicence Heterotrophic denitrification nationallicence Microbial community structure nationallicence 16S rRNA gene nationallicence Xu Guihua Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, 361021, Xiamen, China aut Peng Jingjing Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, 361021, Xiamen, China aut Feng Cuijie Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, 361021, Xiamen, China aut Fang Fang Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, 361021, Xiamen, China aut Chen Shaohua Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, 361021, Xiamen, China aut Xu Yuanjian Key Laboratory of Reservoir Aquatic Environment, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, 400714, Chongqing, China aut Wang Xingzu Key Laboratory of Reservoir Aquatic Environment, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, 400714, Chongqing, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/15(2015-08-01), 6527-6536 0175-7598 99:15<6527 2015 99 253 https://doi.org/10.1007/s00253-015-6532-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6532-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Xu Guihua Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, 361021, Xiamen, China aut NATIONALLICENCE 700 1- Peng Jingjing Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, 361021, Xiamen, China aut NATIONALLICENCE 700 1- Feng Cuijie Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, 361021, Xiamen, China aut NATIONALLICENCE 700 1- Fang Fang Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, 361021, Xiamen, China aut NATIONALLICENCE 700 1- Chen Shaohua Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, 361021, Xiamen, China aut NATIONALLICENCE 700 1- Xu Yuanjian Key Laboratory of Reservoir Aquatic Environment, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, 400714, Chongqing, China aut NATIONALLICENCE 700 1- Wang Xingzu Key Laboratory of Reservoir Aquatic Environment, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, 400714, Chongqing, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/15(2015-08-01), 6527-6536 0175-7598 99:15<6527 2015 99 253