caa a22 4500 605506620 CHVBK 20210128100628.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s00253-015-6580-7 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6580-7 Effect of lake water on algal biomass and microbial community structure in municipal wastewater-based lab-scale photobioreactors [Elektronische Daten] [I. Krustok, J. Truu, M. Odlare, M. Truu, T. Ligi, K. Tiirik, E. Nehrenheim] Photobioreactors are a novel environmental technology that can produce biofuels with the simultaneous removal of nutrients and pollutants from wastewaters. The aim of this study was to evaluate the effect of lake water inoculation on the production of algal biomass and phylogenetic and functional structure of the algal and bacterial communities in municipal wastewater-treating lab-scale photobioreactors. Inoculating the reactors with lake water had a significant benefit to the overall algal biomass growth and nutrient reduction in the reactors with wastewater and lake water (ratio 70/30 v/v). The metagenome-based survey showed that the most abundant algal phylum in these reactors was Chlorophyta with Scenedesmus being the most prominent genus. The most abundant bacterial phyla were Proteobacteria and Bacteroidetes with most dominant families being Sphingobacteriaceae, Cytophagaceae, Flavobacteriaceae, Comamonadaceae, Planctomycetaceae, Nocardiaceae and Nostocaceae. These photobioreactors were also effective in reducing the overall amount of pathogens in wastewater compared to reactors with wastewater/tap water mixture. Functional analysis of the photobioreactor metagenomes revealed an increase in relative abundance genes related to photosynthesis, synthesis of vitamins important for auxotrophic algae and decrease in virulence and nitrogen metabolism subsystems in lake water reactors. The results of the study indicate that adding lake water to the wastewater-based photobioreactor leads to an altered bacterial community phylogenetic and functional structure that could be linked to higher algal biomass production, as well as to enhanced nutrient and pathogen reduction in these reactors. Springer-Verlag Berlin Heidelberg, 2015 Biomass production nationallicence Metagenome analysis nationallicence Photobioreactor nationallicence Wastewater treatment nationallicence Krustok I. School of Business, Society and Engineering, Mälardalen University, P.O. Box 883, SE-721 23, Västerås, Sweden aut Truu J. Institute of Ecology and Earth Sciences, University of Tartu, 46 Vanemuise, 51014, Tartu, Estonia aut Odlare M. School of Business, Society and Engineering, Mälardalen University, P.O. Box 883, SE-721 23, Västerås, Sweden aut Truu M. Institute of Ecology and Earth Sciences, University of Tartu, 46 Vanemuise, 51014, Tartu, Estonia aut Ligi T. Institute of Ecology and Earth Sciences, University of Tartu, 46 Vanemuise, 51014, Tartu, Estonia aut Tiirik K. Institute of Ecology and Earth Sciences, University of Tartu, 46 Vanemuise, 51014, Tartu, Estonia aut Nehrenheim E. School of Business, Society and Engineering, Mälardalen University, P.O. Box 883, SE-721 23, Västerås, Sweden aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/15(2015-08-01), 6537-6549 0175-7598 99:15<6537 2015 99 253 https://doi.org/10.1007/s00253-015-6580-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6580-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Krustok I. School of Business, Society and Engineering, Mälardalen University, P.O. Box 883, SE-721 23, Västerås, Sweden aut NATIONALLICENCE 700 1- Truu J. Institute of Ecology and Earth Sciences, University of Tartu, 46 Vanemuise, 51014, Tartu, Estonia aut NATIONALLICENCE 700 1- Odlare M. School of Business, Society and Engineering, Mälardalen University, P.O. Box 883, SE-721 23, Västerås, Sweden aut NATIONALLICENCE 700 1- Truu M. Institute of Ecology and Earth Sciences, University of Tartu, 46 Vanemuise, 51014, Tartu, Estonia aut NATIONALLICENCE 700 1- Ligi T. Institute of Ecology and Earth Sciences, University of Tartu, 46 Vanemuise, 51014, Tartu, Estonia aut NATIONALLICENCE 700 1- Tiirik K. Institute of Ecology and Earth Sciences, University of Tartu, 46 Vanemuise, 51014, Tartu, Estonia aut NATIONALLICENCE 700 1- Nehrenheim E. School of Business, Society and Engineering, Mälardalen University, P.O. Box 883, SE-721 23, Västerås, Sweden aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/15(2015-08-01), 6537-6549 0175-7598 99:15<6537 2015 99 253