caa a22 4500 605506655 CHVBK 20210128100628.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s00253-015-6586-1 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6586-1 Dynamic investigation of nutrient consumption and injection strategy in microbial enhanced oil recovery (MEOR) by means of large-scale experiments [Elektronische Daten] [Zhiyong Song, Weiyao Zhu, Gangzheng Sun, Koen Blanckaert] Microbial enhanced oil recovery (MEOR) depends on the in situ microbial activity to release trapped oil in reservoirs. In practice, undesired consumption is a universal phenomenon but cannot be observed effectively in small-scale physical simulations due to the scale effect. The present paper investigates the dynamics of oil recovery, biomass and nutrient consumption in a series of flooding experiments in a dedicated large-scale sand-pack column. First, control experiments of nutrient transportation with and without microbial consumption were conducted, which characterized the nutrient loss during transportation. Then, a standard microbial flooding experiment was performed recovering additional oil (4.9% Original Oil in Place, OOIP), during which microbial activity mostly occurred upstream, where oil saturation declined earlier and steeper than downstream in the column. Subsequently, more oil remained downstream due to nutrient shortage. Finally, further research was conducted to enhance the ultimate recovery by optimizing the injection strategy. An extra 3.5% OOIP was recovered when the nutrients were injected in the middle of the column, and another additional 11.9% OOIP were recovered by altering the timing of nutrient injection. Springer-Verlag Berlin Heidelberg, 2015 Microbial enhanced oil recovery nationallicence Nutrient consumption nationallicence Large-scale physical simulation nationallicence Microbial flooding nationallicence Sand-pack column nationallicence Song Zhiyong School of Civil and Environmental Engineering, University of Science and Technology Beijing, 100083, Beijing, China aut Zhu Weiyao School of Civil and Environmental Engineering, University of Science and Technology Beijing, 100083, Beijing, China aut Sun Gangzheng Oil Production Research Institute, Shengli Oil Field Ltd. Co., SinoPEC, 257000, Dongying, China aut Blanckaert Koen ENAC, Ecole Polytechnique Fédérale Lausanne, 1015, Lausanne, Switzerland aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/15(2015-08-01), 6551-6561 0175-7598 99:15<6551 2015 99 253 https://doi.org/10.1007/s00253-015-6586-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6586-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Song Zhiyong School of Civil and Environmental Engineering, University of Science and Technology Beijing, 100083, Beijing, China aut NATIONALLICENCE 700 1- Zhu Weiyao School of Civil and Environmental Engineering, University of Science and Technology Beijing, 100083, Beijing, China aut NATIONALLICENCE 700 1- Sun Gangzheng Oil Production Research Institute, Shengli Oil Field Ltd. Co., SinoPEC, 257000, Dongying, China aut NATIONALLICENCE 700 1- Blanckaert Koen ENAC, Ecole Polytechnique Fédérale Lausanne, 1015, Lausanne, Switzerland aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/15(2015-08-01), 6551-6561 0175-7598 99:15<6551 2015 99 253