Genome-wide investigation of the genes involved in nicotine metabolism in Pseudomonas putida J5 by Tn 5 transposon mutagenesis
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| 024 | 7 | 0 | |a 10.1007/s00253-015-6529-x |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-015-6529-x | ||
| 245 | 0 | 0 | |a Genome-wide investigation of the genes involved in nicotine metabolism in Pseudomonas putida J5 by Tn 5 transposon mutagenesis |h [Elektronische Daten] |c [Zhenyuan Xia, Wei Zhang, Liping Lei, Xingzhong Liu, Hai-Lei Wei] |
| 520 | 3 | |a Pseudomonas putida J5 is an efficient nicotine-degrading bacterial strain isolated from the tobacco rhizosphere. We successfully performed a comprehensive whole-genome analysis of nicotine metabolism-associated genes by Tn5 transposon mutagenesis in P. putida J5. A total of 18 mutants with unique insertions screened from 16,324 Tn5-transformants failed to use nicotine as the sole carbon source. Flanking sequences of the Tn5 transposon were cloned with a shotgun method from all of the nicotine-growth-deficient mutants. The potentially essential products of mutated gene were classified as follows: oxidoreductases, protein and metal transport systems, proteases and peptidases, transcriptional and translational regulators, and unknown proteins. Bioinformatic analysis of the Tn5 insertion sites indicated that the nicotine metabolic genes were separated and widely distributed in the genome. One of the mutants, M2022, was a Tn5 insert into a gene encoding a homolog of 6-hydroxy-l-nicotine oxidase, the second enzyme of nicotine metabolism in Arthrobacter nicotinovorans. Genetic and biochemical analysis confirmed that three open reading frames (ORFs) from an approximately 13-kb fragment recovered from the mutant M2022 were responsible for the transformation of nicotine to 3-succinoyl-pyridine via pseudooxynicotine and 3-succinoyl semialdehyde-pyridine, the first three steps of nicotine degradation. Further research on these mutants and the Tn5-inserted genes will help us characterize nicotine metabolic processes in P. putida J5. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a Nicotine |2 nationallicence | |
| 690 | 7 | |a Pseudomonas putida |2 nationallicence | |
| 690 | 7 | |a Biodegradation |2 nationallicence | |
| 690 | 7 | |a Tn 5 |2 nationallicence | |
| 700 | 1 | |a Xia |D Zhenyuan |u Yunnan Academy of Tobacco Agricultural Science, 650021, Kunming, Yunnan, China |4 aut | |
| 700 | 1 | |a Zhang |D Wei |u Key Laboratory of Agricultural Environment, Ministry of Agriculture, Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, 100081, Beijing, China |4 aut | |
| 700 | 1 | |a Lei |D Liping |u Yunnan Academy of Tobacco Agricultural Science, 650021, Kunming, Yunnan, China |4 aut | |
| 700 | 1 | |a Liu |D Xingzhong |u State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, 3A Datun Rd, Chaoyang District, 100101, Beijing, China |4 aut | |
| 700 | 1 | |a Wei |D Hai-Lei |u State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, 3A Datun Rd, Chaoyang District, 100101, Beijing, China |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/15(2015-08-01), 6503-6514 |x 0175-7598 |q 99:15<6503 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6529-x |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6529-x |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Xia |D Zhenyuan |u Yunnan Academy of Tobacco Agricultural Science, 650021, Kunming, Yunnan, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zhang |D Wei |u Key Laboratory of Agricultural Environment, Ministry of Agriculture, Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, 100081, Beijing, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Lei |D Liping |u Yunnan Academy of Tobacco Agricultural Science, 650021, Kunming, Yunnan, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Liu |D Xingzhong |u State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, 3A Datun Rd, Chaoyang District, 100101, Beijing, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Wei |D Hai-Lei |u State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, 3A Datun Rd, Chaoyang District, 100101, Beijing, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/15(2015-08-01), 6503-6514 |x 0175-7598 |q 99:15<6503 |1 2015 |2 99 |o 253 | ||