caa a22 4500 605506744 CHVBK 20210128100628.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s00253-015-6545-x doi (NATIONALLICENCE)springer-10.1007/s00253-015-6545-x Microbial community analysis of switchgrass planted and unplanted soil microcosms displaying PCB dechlorination [Elektronische Daten] [Yi Liang, Richard Meggo, Dingfei Hu, Jerald Schnoor, Timothy Mattes] Polychlorinated biphenyls (PCBs) pose potential risks to human and environmental health because they are carcinogenic, persistent, and bioaccumulative. In this study, we investigated bacterial communities in soil microcosms spiked with PCB 52, 77, and 153. Switchgrass (Panicum virgatum) was employed to improve overall PCB removal, and redox cycling (i.e., sequential periods of flooding followed by periods of no flooding) was performed in an effort to promote PCB dechlorination. Lesser chlorinated PCB transformation products were detected in all microcosms, indicating the occurrence of PCB dechlorination. Terminal restriction fragment length polymorphism (T-RFLP) and clone library analysis showed that PCB spiking, switchgrass planting, and redox cycling affected the microbial community structure. Putative organohalide-respiring Chloroflexi populations, which were not found in unflooded microcosms, were enriched after 2weeks of flooding in the redox-cycled microcosms. Sequences classified as Geobacter sp. were detected in all microcosms and were most abundant in the switchgrass-planted microcosm spiked with PCB congeners. The presence of possible organohalide-respiring bacteria in these soil microcosms suggests that they play a role in PCB dechlorination therein. Springer-Verlag Berlin Heidelberg, 2015 Chloroflexi nationallicence Geobacter nationallicence Clostridia nationallicence qPCR nationallicence T-RFLP nationallicence Redox cycling nationallicence Phytoremediation nationallicence Switchgrass nationallicence Liang Yi Department of Civil and Environmental Engineering, 4105 Seamans Center, The University of Iowa, 52242, Iowa City, IA, USA aut Meggo Richard Department of Civil and Environmental Engineering, 4105 Seamans Center, The University of Iowa, 52242, Iowa City, IA, USA aut Hu Dingfei Department of Civil and Environmental Engineering, 4105 Seamans Center, The University of Iowa, 52242, Iowa City, IA, USA aut Schnoor Jerald Department of Civil and Environmental Engineering, 4105 Seamans Center, The University of Iowa, 52242, Iowa City, IA, USA aut Mattes Timothy Department of Civil and Environmental Engineering, 4105 Seamans Center, The University of Iowa, 52242, Iowa City, IA, USA aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/15(2015-08-01), 6515-6526 0175-7598 99:15<6515 2015 99 253 https://doi.org/10.1007/s00253-015-6545-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6545-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liang Yi Department of Civil and Environmental Engineering, 4105 Seamans Center, The University of Iowa, 52242, Iowa City, IA, USA aut NATIONALLICENCE 700 1- Meggo Richard Department of Civil and Environmental Engineering, 4105 Seamans Center, The University of Iowa, 52242, Iowa City, IA, USA aut NATIONALLICENCE 700 1- Hu Dingfei Department of Civil and Environmental Engineering, 4105 Seamans Center, The University of Iowa, 52242, Iowa City, IA, USA aut NATIONALLICENCE 700 1- Schnoor Jerald Department of Civil and Environmental Engineering, 4105 Seamans Center, The University of Iowa, 52242, Iowa City, IA, USA aut NATIONALLICENCE 700 1- Mattes Timothy Department of Civil and Environmental Engineering, 4105 Seamans Center, The University of Iowa, 52242, Iowa City, IA, USA aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/15(2015-08-01), 6515-6526 0175-7598 99:15<6515 2015 99 253