caa a22 4500 605506787 CHVBK 20210128100628.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s00253-015-6543-z doi (NATIONALLICENCE)springer-10.1007/s00253-015-6543-z Increased intracellular calcium level and impaired nutrient absorption are important pathogenicity traits in the chicken intestinal epithelium during Campylobacter jejuni colonization [Elektronische Daten] [Wageha Awad, Alina Smorodchenko, Claudia Hess, Jörg Aschenbach, Andor Molnár, Károly Dublecz, Basel Khayal, Elena Pohl, Michael Hess] Although a high number of chickens carry Campylobacter jejuni, the mechanistic action of colonization in the intestine is still poorly understood. The current study was therefore designed to investigate the effects of C. jejuni on glucose uptake, amino acids availability in digesta, and intracellular calcium [Ca2+]i signaling in the intestines of broiler chickens. For this, we compared: control birds (n = 60) and C. jejuni-infected birds (n = 60; infected orally with 1 × 108CFU of C. jejuni NCTC 12744 at 14days of age). Our results showed that glucose uptake was reduced due to C. jejuni infection in isolated jejunal, but not in cecal mucosa at 14days postinfection (dpi). The decrease in intestinal glucose absorption coincided with a decrease in body weight gain during the 2-week post-infectious period. A reduction in the amount of the amino acids (serine, proline, valine, leucine, phenylalanine, arginine, histidine, and lysine) in ileal digesta of the infected birds at 2 and/or 7dpi was found, indicating that Campylobacter utilizes amino acids as a carbon source for their multiplication. Applying the cell-permeable Ca2+ indicator Fluo-4 and two-photon microscopy, we revealed that [Ca2+]i was increased in the jejunal and cecal mucosa of infected birds. The muscarinic agonist carbachol induced an increase in [Ca2+]i in jejunum and cecum mucosa of control chickens, a response absent in the mucosa of infected chickens, demonstrating that the modulation of [Ca2+]i by Campylobacter might be involved in facilitating the necessary cytoskeletal rearrangements that occur during the bacterial invasion of epithelial cells. In conclusion, this study demonstrates the multifaceted interactions of C. jejuni with the gastrointestinal mucosa of broiler chickens. For the first time, it could be shown that a Campylobacter infection could interfere with intracellular Ca2+ signaling and nutrient absorption in the small intestine with consequences on intestinal function, performance, and Campylobacter colonization. Altogether, these findings indicate that Campylobacter is not entirely a commensal and can be recognized as an important factor contributing to an impaired chicken gut health. Springer-Verlag Berlin Heidelberg, 2015 Chickens nationallicence Campylobacter jejuni nationallicence Intestine nationallicence Colonization nationallicence Absorptive function nationallicence Nutrient utilization nationallicence Calcium nationallicence Awad Wageha Clinic for Poultry and Fish Medicine, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine, Vienna, Austria aut Smorodchenko Alina Institute of Veterinary Physiology, Department of Veterinary Medicine, Free University of Berlin, Berlin, Germany aut Hess Claudia Clinic for Poultry and Fish Medicine, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine, Vienna, Austria aut Aschenbach Jörg Institute of Veterinary Physiology, Department of Veterinary Medicine, Free University of Berlin, Berlin, Germany aut Molnár Andor Department of Animal Science and Animal Husbandry, Georgikon Faculty, University of Pannonia, Keszthely, Hungary aut Dublecz Károly Department of Animal Science and Animal Husbandry, Georgikon Faculty, University of Pannonia, Keszthely, Hungary aut Khayal Basel Clinic for Poultry and Fish Medicine, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine, Vienna, Austria aut Pohl Elena Institute of Physiology, Pathophysiology and Biophysics, Department of Biomedical Sciences, University of Veterinary Medicine, Vienna, Austria aut Hess Michael Clinic for Poultry and Fish Medicine, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine, Vienna, Austria aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/15(2015-08-01), 6431-6441 0175-7598 99:15<6431 2015 99 253 https://doi.org/10.1007/s00253-015-6543-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6543-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Awad Wageha Clinic for Poultry and Fish Medicine, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine, Vienna, Austria aut NATIONALLICENCE 700 1- Smorodchenko Alina Institute of Veterinary Physiology, Department of Veterinary Medicine, Free University of Berlin, Berlin, Germany aut NATIONALLICENCE 700 1- Hess Claudia Clinic for Poultry and Fish Medicine, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine, Vienna, Austria aut NATIONALLICENCE 700 1- Aschenbach Jörg Institute of Veterinary Physiology, Department of Veterinary Medicine, Free University of Berlin, Berlin, Germany aut NATIONALLICENCE 700 1- Molnár Andor Department of Animal Science and Animal Husbandry, Georgikon Faculty, University of Pannonia, Keszthely, Hungary aut NATIONALLICENCE 700 1- Dublecz Károly Department of Animal Science and Animal Husbandry, Georgikon Faculty, University of Pannonia, Keszthely, Hungary aut NATIONALLICENCE 700 1- Khayal Basel Clinic for Poultry and Fish Medicine, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine, Vienna, Austria aut NATIONALLICENCE 700 1- Pohl Elena Institute of Physiology, Pathophysiology and Biophysics, Department of Biomedical Sciences, University of Veterinary Medicine, Vienna, Austria aut NATIONALLICENCE 700 1- Hess Michael Clinic for Poultry and Fish Medicine, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine, Vienna, Austria aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/15(2015-08-01), 6431-6441 0175-7598 99:15<6431 2015 99 253