Ammonia manipulates the ammonia-oxidizing archaea and bacteria in the coastal sediment-water microcosms
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| 024 | 7 | 0 | |a 10.1007/s00253-015-6524-2 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-015-6524-2 | ||
| 245 | 0 | 0 | |a Ammonia manipulates the ammonia-oxidizing archaea and bacteria in the coastal sediment-water microcosms |h [Elektronische Daten] |c [Yan Zhang, Lujun Chen, Tianjiao Dai, Renhua Sun, Donghui Wen] |
| 520 | 3 | |a Ammonia was observed as a potential significant factor to manipulate the abundance and activity of ammonia-oxidizing microorganisms (AOMs) in water environments. For the first time, this study confirmed this phenomenon by laboratory cultivation. In a series of estuarine sediment-coastal water microcosms, we investigated the AOM's phylogenetic composition and activity change in response to ammonia concentration. Increase of ammonia concentration promoted bacterial amoA gene abundance in a linear pattern. The ratio of transcribed ammonia-oxidizing bacteria (AOB) amoA gene/ammonia-oxidizing archaea (AOA) amoA gene increased from 0.1 to 43 as NH4 +-N increased from less than 0.1 to 12mgL−1, and AOA amoA transcription was undetected under 20mg NH4 +-NL−1. The incubation of stable isotope probing (SIP) microcosms revealed a faster 13C-NaHCO3 incorporation rate of AOA amoA gene under 0.1mg NH4 +-NL−1 and a sole 13C-NaHCO3 utilization of the AOB amoA gene under 20mg NH4 +-NL−1. Our results indicate that ammonia concentration manipulates the structure of AOM. AOA prefers to live and perform higher amoA transcription activity than AOB in ammonia-limited water environments, and AOB tends to take the first contributor place in ammonia-rich ones. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a Ammonia-oxidizing archaea |2 nationallicence | |
| 690 | 7 | |a Ammonia-oxidizing bacteria |2 nationallicence | |
| 690 | 7 | |a Sediment microcosm |2 nationallicence | |
| 690 | 7 | |a Stable isotope probing |2 nationallicence | |
| 690 | 7 | |a Transcriptional activity |2 nationallicence | |
| 700 | 1 | |a Zhang |D Yan |u School of Environment, Tsinghua University, 100084, Beijing, China |4 aut | |
| 700 | 1 | |a Chen |D Lujun |u School of Environment, Tsinghua University, 100084, Beijing, China |4 aut | |
| 700 | 1 | |a Dai |D Tianjiao |u College of Environmental Sciences and Engineering, Peking University, 100871, Beijing, China |4 aut | |
| 700 | 1 | |a Sun |D Renhua |u College of Environmental Sciences and Engineering, Peking University, 100871, Beijing, China |4 aut | |
| 700 | 1 | |a Wen |D Donghui |u College of Environmental Sciences and Engineering, Peking University, 100871, Beijing, China |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/15(2015-08-01), 6481-6491 |x 0175-7598 |q 99:15<6481 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6524-2 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6524-2 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zhang |D Yan |u School of Environment, Tsinghua University, 100084, Beijing, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Chen |D Lujun |u School of Environment, Tsinghua University, 100084, Beijing, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Dai |D Tianjiao |u College of Environmental Sciences and Engineering, Peking University, 100871, Beijing, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Sun |D Renhua |u College of Environmental Sciences and Engineering, Peking University, 100871, Beijing, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Wen |D Donghui |u College of Environmental Sciences and Engineering, Peking University, 100871, Beijing, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/15(2015-08-01), 6481-6491 |x 0175-7598 |q 99:15<6481 |1 2015 |2 99 |o 253 | ||