caa a22 4500 605506795 CHVBK 20210128100629.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s00253-015-6524-2 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6524-2 Ammonia manipulates the ammonia-oxidizing archaea and bacteria in the coastal sediment-water microcosms [Elektronische Daten] [Yan Zhang, Lujun Chen, Tianjiao Dai, Renhua Sun, Donghui Wen] Ammonia was observed as a potential significant factor to manipulate the abundance and activity of ammonia-oxidizing microorganisms (AOMs) in water environments. For the first time, this study confirmed this phenomenon by laboratory cultivation. In a series of estuarine sediment-coastal water microcosms, we investigated the AOM's phylogenetic composition and activity change in response to ammonia concentration. Increase of ammonia concentration promoted bacterial amoA gene abundance in a linear pattern. The ratio of transcribed ammonia-oxidizing bacteria (AOB) amoA gene/ammonia-oxidizing archaea (AOA) amoA gene increased from 0.1 to 43 as NH4 +-N increased from less than 0.1 to 12mgL−1, and AOA amoA transcription was undetected under 20mg NH4 +-NL−1. The incubation of stable isotope probing (SIP) microcosms revealed a faster 13C-NaHCO3 incorporation rate of AOA amoA gene under 0.1mg NH4 +-NL−1 and a sole 13C-NaHCO3 utilization of the AOB amoA gene under 20mg NH4 +-NL−1. Our results indicate that ammonia concentration manipulates the structure of AOM. AOA prefers to live and perform higher amoA transcription activity than AOB in ammonia-limited water environments, and AOB tends to take the first contributor place in ammonia-rich ones. Springer-Verlag Berlin Heidelberg, 2015 Ammonia-oxidizing archaea nationallicence Ammonia-oxidizing bacteria nationallicence Sediment microcosm nationallicence Stable isotope probing nationallicence Transcriptional activity nationallicence Zhang Yan School of Environment, Tsinghua University, 100084, Beijing, China aut Chen Lujun School of Environment, Tsinghua University, 100084, Beijing, China aut Dai Tianjiao College of Environmental Sciences and Engineering, Peking University, 100871, Beijing, China aut Sun Renhua College of Environmental Sciences and Engineering, Peking University, 100871, Beijing, China aut Wen Donghui College of Environmental Sciences and Engineering, Peking University, 100871, Beijing, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/15(2015-08-01), 6481-6491 0175-7598 99:15<6481 2015 99 253 https://doi.org/10.1007/s00253-015-6524-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6524-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Yan School of Environment, Tsinghua University, 100084, Beijing, China aut NATIONALLICENCE 700 1- Chen Lujun School of Environment, Tsinghua University, 100084, Beijing, China aut NATIONALLICENCE 700 1- Dai Tianjiao College of Environmental Sciences and Engineering, Peking University, 100871, Beijing, China aut NATIONALLICENCE 700 1- Sun Renhua College of Environmental Sciences and Engineering, Peking University, 100871, Beijing, China aut NATIONALLICENCE 700 1- Wen Donghui College of Environmental Sciences and Engineering, Peking University, 100871, Beijing, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/15(2015-08-01), 6481-6491 0175-7598 99:15<6481 2015 99 253