caa a22 4500 605506809 CHVBK 20210128100629.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s00253-015-6523-3 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6523-3 Extracellular esterases of phylloplane yeast Pseudozyma antarctica induce defect on cuticle layer structure and water-holding ability of plant leaves [Elektronische Daten] [Hirokazu Ueda, Ichiro Mitsuhara, Jun Tabata, Soichi Kugimiya, Takashi Watanabe, Ken Suzuki, Shigenobu Yoshida, Hiroko Kitamoto] Aerial plant surface (phylloplane) is a primary key habitat for many microorganisms but is generally recognized as limited in nutrient resources. Pseudozyma antarctica, a nonpathogenic yeast, is commonly isolated from plant surfaces and characterized as an esterase producer with fatty acid assimilation ability. In order to elucidate the biological functions of these esterases, culture filtrate with high esterase activity (crude enzyme) of P. antarctica was applied onto leaves of tomato and Arabidopsis. These leaves showed a wilty phenotype, which is typically associated with water deficiency. Furthermore, we confirmed that crude enzyme-treated detached leaves clearly lost their water-holding ability. In treated leaves of both plants, genes associated to abscisic acid (ABA; a plant stress hormone responding osmotic stress) were activated and accumulation of ABA was confirmed in tomato plants. Microscopic observation of treated leaf surfaces revealed that cuticle layer covering the aerial epidermis of leaves became thinner. A gas chromatography-mass spectrometry (GC-MS) analysis exhibited that fatty acids with 16 and 18 carbon chains were released in larger amounts from treated leaf surfaces, indicating that the crude enzyme has ability to degrade lipid components of cuticle layer. Among the three esterases detected in the crude enzyme, lipase A, lipase B, and P. antarctica esterase (PaE), an in vitro enzyme assay using para-nitrophenyl palmitate as substrate demonstrated that PaE was the most responsible for the degradation. These results suggest that PaE has a potential role in the extraction of fatty acids from plant surfaces, making them available for the growth of phylloplane yeasts. Springer-Verlag Berlin Heidelberg, 2015 Pseudozyma antarctica nationallicence Plant surface nationallicence Cuticle layer nationallicence Biodegradable plastic-degrading enzyme nationallicence Drought stress response nationallicence Ueda Hirokazu National Institute for Agro-Environmental Sciences (NIAES), 3-1-3 Kannondai, 305-8604, Tsukuba, Ibaraki, Japan aut Mitsuhara Ichiro National Institute of Agrobiological Sciences (NIAS), 2-1-2 Kannondai, 305-8602, Tsukuba, Ibaraki, Japan aut Tabata Jun National Institute for Agro-Environmental Sciences (NIAES), 3-1-3 Kannondai, 305-8604, Tsukuba, Ibaraki, Japan aut Kugimiya Soichi National Institute for Agro-Environmental Sciences (NIAES), 3-1-3 Kannondai, 305-8604, Tsukuba, Ibaraki, Japan aut Watanabe Takashi National Institute for Agro-Environmental Sciences (NIAES), 3-1-3 Kannondai, 305-8604, Tsukuba, Ibaraki, Japan aut Suzuki Ken National Institute for Agro-Environmental Sciences (NIAES), 3-1-3 Kannondai, 305-8604, Tsukuba, Ibaraki, Japan aut Yoshida Shigenobu National Institute for Agro-Environmental Sciences (NIAES), 3-1-3 Kannondai, 305-8604, Tsukuba, Ibaraki, Japan aut Kitamoto Hiroko National Institute for Agro-Environmental Sciences (NIAES), 3-1-3 Kannondai, 305-8604, Tsukuba, Ibaraki, Japan aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/15(2015-08-01), 6405-6415 0175-7598 99:15<6405 2015 99 253 https://doi.org/10.1007/s00253-015-6523-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6523-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ueda Hirokazu National Institute for Agro-Environmental Sciences (NIAES), 3-1-3 Kannondai, 305-8604, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 700 1- Mitsuhara Ichiro National Institute of Agrobiological Sciences (NIAS), 2-1-2 Kannondai, 305-8602, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 700 1- Tabata Jun National Institute for Agro-Environmental Sciences (NIAES), 3-1-3 Kannondai, 305-8604, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 700 1- Kugimiya Soichi National Institute for Agro-Environmental Sciences (NIAES), 3-1-3 Kannondai, 305-8604, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 700 1- Watanabe Takashi National Institute for Agro-Environmental Sciences (NIAES), 3-1-3 Kannondai, 305-8604, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 700 1- Suzuki Ken National Institute for Agro-Environmental Sciences (NIAES), 3-1-3 Kannondai, 305-8604, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 700 1- Yoshida Shigenobu National Institute for Agro-Environmental Sciences (NIAES), 3-1-3 Kannondai, 305-8604, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 700 1- Kitamoto Hiroko National Institute for Agro-Environmental Sciences (NIAES), 3-1-3 Kannondai, 305-8604, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/15(2015-08-01), 6405-6415 0175-7598 99:15<6405 2015 99 253