caa a22 4500 60550685X CHVBK 20210128100629.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s00775-014-1206-6 doi (NATIONALLICENCE)springer-10.1007/s00775-014-1206-6 Effective selenium detoxification in the seed proteins of a hyperaccumulator plant: the analysis of selenium-containing proteins of monkeypot nut ( Lecythis minor ) seeds [Elektronische Daten] [Anikó Németh, Mihály Dernovics] A shotgun proteomic approach was applied to characterize the selenium (Se)-containing proteins of the selenium hyperaccumulator monkeypot nut (Lecythis minor) seeds. The exceptionally high Se content (>4,000mgkg−1) of the sample enabled a straightforward procedure without the need for multiple preconcentration and fractionation steps. The proteins identified were sulfur-rich seed proteins, namely, 11S globulin (Q84ND2), 2S albumin (B6EU54), 2S sulfur-rich seed storage proteins (P04403 and P0C8Y8) and a 11S globulin-like protein (A0EM48). Database directed search for theoretically selenium-containing peptides was assisted by manual spectra evaluation to achieve around 25% coverage on sulfur analogues. Remarkable detoxification mechanisms on the proteome level were revealed in the form of multiple selenomethionine-methionine substitution and the lack of selenocysteine residues. The degree of selenomethionine substitution could be characterized by an exponential function that implies the inhibition of protein elongation by selenomethionine. Our results contribute to the deeper understanding of selenium detoxification procedures in hyperaccumulator plants. SBIC, 2014 Selenium nationallicence Hyperaccumulator nationallicence Multiple S/Se substitution nationallicence Németh Anikó Department of Applied Chemistry, Faculty of Food Science, Corvinus University of Budapest, Villányi út 29-33, 1118, Budapest, Hungary aut Dernovics Mihály Department of Applied Chemistry, Faculty of Food Science, Corvinus University of Budapest, Villányi út 29-33, 1118, Budapest, Hungary aut JBIC Journal of Biological Inorganic Chemistry Springer Berlin Heidelberg 20/1(2015-01-01), 23-33 0949-8257 20:1<23 2015 20 775 https://doi.org/10.1007/s00775-014-1206-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00775-014-1206-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Németh Anikó Department of Applied Chemistry, Faculty of Food Science, Corvinus University of Budapest, Villányi út 29-33, 1118, Budapest, Hungary aut NATIONALLICENCE 700 1- Dernovics Mihály Department of Applied Chemistry, Faculty of Food Science, Corvinus University of Budapest, Villányi út 29-33, 1118, Budapest, Hungary aut NATIONALLICENCE 773 0- JBIC Journal of Biological Inorganic Chemistry Springer Berlin Heidelberg 20/1(2015-01-01), 23-33 0949-8257 20:1<23 2015 20 775