caa a22 4500 605506914 CHVBK 20210128100629.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s00775-014-1221-7 doi (NATIONALLICENCE)springer-10.1007/s00775-014-1221-7 Phosphonomethyl iminodiacetic acid-conjugated cobalt oxide nanoparticles liberate Co++ ion-induced stress associated activation of TNF-α/p38 MAPK/caspase 8-caspase 3 signaling in human leukemia cells [Elektronische Daten] [Sourav Chattopadhyay, Sandeep Dash, Satyajit Tripathy, Panchanan Pramanik, Somenath Roy] The aim of this work is to understand the potential health effects of metal nanoparticles by exposing human leukemic cell lines (jurkat, K562 and KG1A cells) to nanosize phosphonomethyl iminodiacetic acid coated cobalt oxide (PMIDA-CoO) NPs. The synthesized PMIDA-CoO NPs were characterized by XRD, dynamic light scattering, transmission electron microscopy and scanning electron microscopy. Our results showed that exposure of leukemic cell lines to PMIDA-CoO NPs caused reactive oxygen species (ROS) generation by increasing the concentration of free Co++ ions in cancer microenvironment. But at physiological pH, PMIDA-CoO liberates little amount of Co++ ions into media and exerts lower toxicity to normal cells up to a certain dose. PMIDA-CoO NPs caused DNA damage in leukemic cell lines, which was reflected by an increase in apoptosis of jurkat, KG-1A and K562 cells. PMIDA-CoO NPs induced apoptosis by increasing pro-inflammatory cytokines, primarily TNF-α. The in vivo study shows that PMIDA-CoO NPs were efficiently killed DLA cells. These findings have important implications for understanding the potential anticancer property induced by surface-modified cobalt oxide nanoparticles. SBIC, 2014 Cobalt nanoparticles nationallicence PMIDA nationallicence Cytotoxicity nationallicence Anticancer activity nationallicence Cytokines nationallicence Chattopadhyay Sourav Department of Human Physiology with Community Health, Immunology and Microbiology Laboratory, Vidyasagar University, Midnapore, 721 102, West Bengal, India aut Dash Sandeep Department of Human Physiology with Community Health, Immunology and Microbiology Laboratory, Vidyasagar University, Midnapore, 721 102, West Bengal, India aut Tripathy Satyajit Department of Human Physiology with Community Health, Immunology and Microbiology Laboratory, Vidyasagar University, Midnapore, 721 102, West Bengal, India aut Pramanik Panchanan Department of Chemistry, Nano Materials Laboratory, Indian Institute of Technology, Khargapur, West Bengal, India aut Roy Somenath Department of Human Physiology with Community Health, Immunology and Microbiology Laboratory, Vidyasagar University, Midnapore, 721 102, West Bengal, India aut JBIC Journal of Biological Inorganic Chemistry Springer Berlin Heidelberg 20/1(2015-01-01), 123-141 0949-8257 20:1<123 2015 20 775 https://doi.org/10.1007/s00775-014-1221-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00775-014-1221-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chattopadhyay Sourav Department of Human Physiology with Community Health, Immunology and Microbiology Laboratory, Vidyasagar University, Midnapore, 721 102, West Bengal, India aut NATIONALLICENCE 700 1- Dash Sandeep Department of Human Physiology with Community Health, Immunology and Microbiology Laboratory, Vidyasagar University, Midnapore, 721 102, West Bengal, India aut NATIONALLICENCE 700 1- Tripathy Satyajit Department of Human Physiology with Community Health, Immunology and Microbiology Laboratory, Vidyasagar University, Midnapore, 721 102, West Bengal, India aut NATIONALLICENCE 700 1- Pramanik Panchanan Department of Chemistry, Nano Materials Laboratory, Indian Institute of Technology, Khargapur, West Bengal, India aut NATIONALLICENCE 700 1- Roy Somenath Department of Human Physiology with Community Health, Immunology and Microbiology Laboratory, Vidyasagar University, Midnapore, 721 102, West Bengal, India aut NATIONALLICENCE 773 0- JBIC Journal of Biological Inorganic Chemistry Springer Berlin Heidelberg 20/1(2015-01-01), 123-141 0949-8257 20:1<123 2015 20 775