caa a22 4500 605506922 CHVBK 20210128100629.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s00775-014-1202-x doi (NATIONALLICENCE)springer-10.1007/s00775-014-1202-x Direct determination of the binding sites of cisplatin on insulin-like growth factor-1 by top-down mass spectrometry [Elektronische Daten] [Ningbo Zhang, Huan Liu, Meng Cui, Yonggang Du, Zhiqiang Liu, Shuying Liu] Cisplatin has been widely used in the chemotherapy of a variety of tumors, and the interactions of cisplatin with proteins play very important roles in its side effects and drug resistance, as well as its pharmacokinetics and the biodistribution. Insulin-like growth factor-1 (IGF-1) was found to be associated with the drug resistance of cisplatin. Here, the interaction between cisplatin and IGF-1 was investigated using electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry. IGF-1-Pt(NH3)Cl was the main mono-adduct and the trans labilization was important to the reaction between IGF-1 and cisplatin, while another special mono-adduct IGF-1-Pt(NH3)Cl2 was observed. The rapid and sensitive top-down mass spectrometry-based approach in linear ion trap mass spectrometer has been developed to identify the binding sites of cisplatin in IGF-1 directly without tedious enzyme digestion. Three binding sites (Met59, Arg56 and Cys6) of cisplatin in IGF-1 were determined. The results not only provide a rapid and efficient way to identify the platinum binding sites in proteins, but also indicate that the binding of cisplatin could promote the fragmentation of IGF-1 and the rupture of disulfide bond. SBIC, 2014 Insulin-like growth factor-1 nationallicence Anticancer drug nationallicence Top-down nationallicence Mass spectrometry nationallicence Zhang Ningbo Changchun Center of Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, 130022, Changchun, People's Republic of China aut Liu Huan Changchun Center of Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, 130022, Changchun, People's Republic of China aut Cui Meng Changchun Center of Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, 130022, Changchun, People's Republic of China aut Du Yonggang Changchun Center of Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, 130022, Changchun, People's Republic of China aut Liu Zhiqiang Changchun Center of Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, 130022, Changchun, People's Republic of China aut Liu Shuying Changchun Center of Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, 130022, Changchun, People's Republic of China aut JBIC Journal of Biological Inorganic Chemistry Springer Berlin Heidelberg 20/1(2015-01-01), 1-10 0949-8257 20:1<1 2015 20 775 https://doi.org/10.1007/s00775-014-1202-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00775-014-1202-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Ningbo Changchun Center of Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, 130022, Changchun, People's Republic of China aut NATIONALLICENCE 700 1- Liu Huan Changchun Center of Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, 130022, Changchun, People's Republic of China aut NATIONALLICENCE 700 1- Cui Meng Changchun Center of Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, 130022, Changchun, People's Republic of China aut NATIONALLICENCE 700 1- Du Yonggang Changchun Center of Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, 130022, Changchun, People's Republic of China aut NATIONALLICENCE 700 1- Liu Zhiqiang Changchun Center of Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, 130022, Changchun, People's Republic of China aut NATIONALLICENCE 700 1- Liu Shuying Changchun Center of Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, 130022, Changchun, People's Republic of China aut NATIONALLICENCE 773 0- JBIC Journal of Biological Inorganic Chemistry Springer Berlin Heidelberg 20/1(2015-01-01), 1-10 0949-8257 20:1<1 2015 20 775