caa a22 4500 605507023 CHVBK 20210128100630.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s00775-015-1281-3 doi (NATIONALLICENCE)springer-10.1007/s00775-015-1281-3 Elemental mapping of the entire intact Drosophila gastrointestinal tract [Elektronische Daten] [Michael Jones, Martin de Jonge, Simon James, Richard Burke] The main role of the animal gastrointestinal (GI) tract is the selective absorption of dietary nutrients from ingested food sources. One class of vital micronutrients are the essential biometals such as copper, zinc and iron, which participate in a plethora of biological process, acting as enzymatic or structural co-factors for numerous proteins and also as important cellular signalling molecules. To help elucidate the mechanisms by which biometals are absorbed from the diet, we mapped elemental distribution in entire, intact Drosophila larval GI tracts using synchrotron X-ray fluorescence microscopy. Our results revealed distinct regions of the GI tract enriched for specific metals. Copper was found to be concentrated in the copper cell region but also in the region directly anterior to the copper cells and unexpectedly, in the middle midgut/iron cell region as well. Iron was observed exclusively in the iron cell region, confirming previous work with iron-specific histological stains. Zinc was observed throughout the GI tract with an increased accumulation in the posterior midgut region, while manganese was seen to co-localize with calcium specifically in clusters in the distal Malpighian tubules. This work simultaneously reveals distribution of a number of biologically important elements in entire, intact GI tracts. These distributions revealed not only a previously undescribed Ca/Mn co-localization, but also the unexpected presence of additional Cu accumulations in the iron cell region. SBIC, 2015 Drosophila nationallicence Metal ion absorption nationallicence Gastrointestinal tract nationallicence Synchrotron nationallicence X-ray fluorescence microscopy nationallicence Jones Michael Australian Synchrotron, 800 Blackburn Road, 3168, Clayton, Australia aut de Jonge Martin Australian Synchrotron, 800 Blackburn Road, 3168, Clayton, Australia aut James Simon Australian Synchrotron, 800 Blackburn Road, 3168, Clayton, Australia aut Burke Richard School of Biological Sciences, Monash University, Wellington Road, 3163, Clayton, Australia aut JBIC Journal of Biological Inorganic Chemistry Springer Berlin Heidelberg 20/6(2015-09-01), 979-987 0949-8257 20:6<979 2015 20 775 https://doi.org/10.1007/s00775-015-1281-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00775-015-1281-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jones Michael Australian Synchrotron, 800 Blackburn Road, 3168, Clayton, Australia aut NATIONALLICENCE 700 1- de Jonge Martin Australian Synchrotron, 800 Blackburn Road, 3168, Clayton, Australia aut NATIONALLICENCE 700 1- James Simon Australian Synchrotron, 800 Blackburn Road, 3168, Clayton, Australia aut NATIONALLICENCE 700 1- Burke Richard School of Biological Sciences, Monash University, Wellington Road, 3163, Clayton, Australia aut NATIONALLICENCE 773 0- JBIC Journal of Biological Inorganic Chemistry Springer Berlin Heidelberg 20/6(2015-09-01), 979-987 0949-8257 20:6<979 2015 20 775