caa a22 4500 605507074 CHVBK 20210128100630.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s00775-015-1276-0 doi (NATIONALLICENCE)springer-10.1007/s00775-015-1276-0 Lysozyme stability and amyloid fibrillization dependence on Hofmeister anions in acidic pH [Elektronische Daten] [Slavomíra Poniková, Andrea Antošová, Erna Demjén, Dagmar Sedláková, Jozef Marek, Rastislav Varhač, Zuzana Gažová, Erik Sedlák] We have explored an effect of Hofmeister anions, Na2SO4, NaCl, NaBr, NaNO3, NaSCN and NaClO4, on stability and amyloid fibrillization of hen egg white lysozyme at pH 2.7. The stability of the protein was analyzed by differential scanning calorimetry. The Hofmeister effect of the anions was assessed by the parameter dT trs/d[anion] (T trs, transition temperature). We show that dT trs/d[anion] correlates with anion surface tension effects and anion partition coefficients indicating direct interactions between anions and lysozyme. The kinetic of amyloid fibrillization of lysozyme was followed by Thioflavin T (ThT) fluorescence. Negative correlation between dT trs/d[anion] and the nucleation rate of fibrillization in the presence of monovalent anions indicates specific effect of anions on fibrillization rate of lysozyme. The efficiency of monovalent anions to accelerate fibrillization correlates with inverse Hofmeister series. The far-UV circular dichroism spectroscopy and atomic force microscopy findings show that conformational properties of fibrils depend on fibrillization rate. In the presence of sodium chloride, lysozyme forms typical fibrils with elongated structure and with the secondary structure of the β-sheet. On the other hand, in the presence of both chaotropic perchlorate and kosmotropic sulfate anions, the fibrils form clusters with secondary structure of β-turn. Moreover, the acceleration of fibril formation is accompanied by decreased amount of the formed fibrils as indicated by ThT fluorescence. Taken together, our study shows Hofmeister effect of monovalent anions on: (1) lysozyme stability; (2) ability to accelerate nucleation phase of lysozyme fibrillization; (3) amount, and (4) conformational properties of the formed fibrils. SBIC, 2015 Protein stability nationallicence Acidic protein nationallicence Hofmeister series nationallicence Fibrillization kinetics nationallicence Poniková Slavomíra Department of Biophysics, Institute of Experimental Physics Slovak Academy of Sciences, Watsonova 47, 04001, Košice, Slovakia aut Antošová Andrea Department of Biophysics, Institute of Experimental Physics Slovak Academy of Sciences, Watsonova 47, 04001, Košice, Slovakia aut Demjén Erna Department of Biophysics, Institute of Experimental Physics Slovak Academy of Sciences, Watsonova 47, 04001, Košice, Slovakia aut Sedláková Dagmar Department of Biophysics, Institute of Experimental Physics Slovak Academy of Sciences, Watsonova 47, 04001, Košice, Slovakia aut Marek Jozef Department of Biophysics, Institute of Experimental Physics Slovak Academy of Sciences, Watsonova 47, 04001, Košice, Slovakia aut Varhač Rastislav Department of Biochemistry, P.J. Šafárik University, Moyzesova 11, 04001, Košice, Slovakia aut Gažová Zuzana Department of Biophysics, Institute of Experimental Physics Slovak Academy of Sciences, Watsonova 47, 04001, Košice, Slovakia aut Sedlák Erik Department of Biochemistry, P.J. Šafárik University, Moyzesova 11, 04001, Košice, Slovakia aut JBIC Journal of Biological Inorganic Chemistry Springer Berlin Heidelberg 20/6(2015-09-01), 921-933 0949-8257 20:6<921 2015 20 775 https://doi.org/10.1007/s00775-015-1276-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00775-015-1276-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Poniková Slavomíra Department of Biophysics, Institute of Experimental Physics Slovak Academy of Sciences, Watsonova 47, 04001, Košice, Slovakia aut NATIONALLICENCE 700 1- Antošová Andrea Department of Biophysics, Institute of Experimental Physics Slovak Academy of Sciences, Watsonova 47, 04001, Košice, Slovakia aut NATIONALLICENCE 700 1- Demjén Erna Department of Biophysics, Institute of Experimental Physics Slovak Academy of Sciences, Watsonova 47, 04001, Košice, Slovakia aut NATIONALLICENCE 700 1- Sedláková Dagmar Department of Biophysics, Institute of Experimental Physics Slovak Academy of Sciences, Watsonova 47, 04001, Košice, Slovakia aut NATIONALLICENCE 700 1- Marek Jozef Department of Biophysics, Institute of Experimental Physics Slovak Academy of Sciences, Watsonova 47, 04001, Košice, Slovakia aut NATIONALLICENCE 700 1- Varhač Rastislav Department of Biochemistry, P.J. Šafárik University, Moyzesova 11, 04001, Košice, Slovakia aut NATIONALLICENCE 700 1- Gažová Zuzana Department of Biophysics, Institute of Experimental Physics Slovak Academy of Sciences, Watsonova 47, 04001, Košice, Slovakia aut NATIONALLICENCE 700 1- Sedlák Erik Department of Biochemistry, P.J. Šafárik University, Moyzesova 11, 04001, Košice, Slovakia aut NATIONALLICENCE 773 0- JBIC Journal of Biological Inorganic Chemistry Springer Berlin Heidelberg 20/6(2015-09-01), 921-933 0949-8257 20:6<921 2015 20 775