caa a22 4500 605507082 CHVBK 20210128100630.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s00775-015-1280-4 doi (NATIONALLICENCE)springer-10.1007/s00775-015-1280-4 Water-soluble lipophilic MR contrast agents for cell membrane labeling [Elektronische Daten] [Christiane Carney, Keith MacRenaris, Thomas Meade] Long-term cell tracking using MR imaging necessitates the development of contrast agents that both label and are retained by cells. One promising strategy for long-term cell labeling is the development of lipophilic Gd(III)-based contrast agents that anchor into the cell membrane. We have previously reported the efficacy of monomeric and multimeric lipophilic agents and showed that the monomeric agents have improved labeling and contrast enhancement of cell populations. Here, we report on the synthesis, characterization, and in vitro testing of a series of monomeric lipophilic contrast agents with varied alkyl chain compositions. We show that these agents disperse in water, localize to the cell membrane, and label HeLa and MCF7 cells effectively. Additionally, these agents have up to tenfold improved retention in cells compared to clinically available ProHance®. SBIC, 2015 MRI nationallicence Contrast agents nationallicence Gadolinium nationallicence Cell membrane nationallicence Cell labeling nationallicence Carney Christiane Department of Chemistry, Molecular Biosciences, Neurobiology, Biomedical Engineering, and Radiology, Northwestern University, 2145 Sheridan Road, 60208-3113, Evanston, IL, USA aut MacRenaris Keith Department of Chemistry, Molecular Biosciences, Neurobiology, Biomedical Engineering, and Radiology, Northwestern University, 2145 Sheridan Road, 60208-3113, Evanston, IL, USA aut Meade Thomas Department of Chemistry, Molecular Biosciences, Neurobiology, Biomedical Engineering, and Radiology, Northwestern University, 2145 Sheridan Road, 60208-3113, Evanston, IL, USA aut JBIC Journal of Biological Inorganic Chemistry Springer Berlin Heidelberg 20/6(2015-09-01), 971-977 0949-8257 20:6<971 2015 20 775 https://doi.org/10.1007/s00775-015-1280-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00775-015-1280-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Carney Christiane Department of Chemistry, Molecular Biosciences, Neurobiology, Biomedical Engineering, and Radiology, Northwestern University, 2145 Sheridan Road, 60208-3113, Evanston, IL, USA aut NATIONALLICENCE 700 1- MacRenaris Keith Department of Chemistry, Molecular Biosciences, Neurobiology, Biomedical Engineering, and Radiology, Northwestern University, 2145 Sheridan Road, 60208-3113, Evanston, IL, USA aut NATIONALLICENCE 700 1- Meade Thomas Department of Chemistry, Molecular Biosciences, Neurobiology, Biomedical Engineering, and Radiology, Northwestern University, 2145 Sheridan Road, 60208-3113, Evanston, IL, USA aut NATIONALLICENCE 773 0- JBIC Journal of Biological Inorganic Chemistry Springer Berlin Heidelberg 20/6(2015-09-01), 971-977 0949-8257 20:6<971 2015 20 775