Effects of the ruthenium-based drug NAMI-A on the roles played by TGF-β1 in the metastatic process
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| 024 | 7 | 0 | |a 10.1007/s00775-015-1297-8 |2 doi |
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| 245 | 0 | 0 | |a Effects of the ruthenium-based drug NAMI-A on the roles played by TGF-β1 in the metastatic process |h [Elektronische Daten] |c [L. Brescacin, A. Masi, G. Sava, A. Bergamo] |
| 520 | 3 | |a The ruthenium-based drug NAMI-A, characterised by its selectivity against solid tumour metastases, promotes TGF-β1-dependent fibrosis and the reduction of the release of MMPs in the primary tumour. The aim of the study was to examine the interaction of NAMI-A with TGF-β1 in the process of metastasis formation. NAMI-A (1) affects the secretion of TGF-β1 in metastatic MDA-MB-231 cells rather than in non-tumorigenic HBL-100 cells, (2) prevails over TGF-β1 with regard to the invasive capacity of the treated cells, and (3) contrasts integrin-dependent migration stimulated by TGF-β1. It, thus, appears that the effects of NAMI-A on cell invasion and migration are best summarised as an interference with TGF-β1 and a reduction of its activity in these events. At a molecular level, the similar activity of NAMI-A and TGF-β1 on RhoA GTPase supports its interaction with cell surface integrins while TGF-β1 can activate it by interaction with its TGFβR receptor. The inhibition of TGF-β1-induced migration of MDA-MB-231 cells by NAMI-A cannot simply be attributed to a modulation of the Smad2 and p38MAPK pathways. In conclusion, the effects of NAMI-A on the biological role of TGF-β1 in cancer metastasis are insufficient to attribute the responsibility for the anti-metastatic activity of the ruthenium-based drug to this target alone. Graphical Abstract: | |
| 540 | |a SBIC, 2015 | ||
| 690 | 7 | |a Anticancer drug |2 nationallicence | |
| 690 | 7 | |a Heavy metal |2 nationallicence | |
| 690 | 7 | |a Biomedicine |2 nationallicence | |
| 690 | 7 | |a Molecular dynamics |2 nationallicence | |
| 690 | 7 | |a BSA : Bovine serum albumin |2 nationallicence | |
| 690 | 7 | |a CHK1 : Checkpoint kinase 1 |2 nationallicence | |
| 690 | 7 | |a CMF-DPBS : Calcium and Magnesium Free Dulbecco's Phosphate Buffered Saline |2 nationallicence | |
| 690 | 7 | |a ECM : Extracellular matrix |2 nationallicence | |
| 690 | 7 | |a DMEM : Dulbecco's modified Eagle's medium |2 nationallicence | |
| 690 | 7 | |a DPBS : Dulbecco's Phosphate Buffered Saline |2 nationallicence | |
| 690 | 7 | |a FBS : Foetal bovine serum |2 nationallicence | |
| 690 | 7 | |a GRP78 : 78kDa glucose-regulated protein |2 nationallicence | |
| 690 | 7 | |a LB : Lysis buffer |2 nationallicence | |
| 690 | 7 | |a MMPs : Matrix metallo proteinases |2 nationallicence | |
| 690 | 7 | |a NAMI-A : Imidazolium trans-imidazoletetrachlororuthenate |2 nationallicence | |
| 690 | 7 | |a ROS : Reactive oxygen species |2 nationallicence | |
| 690 | 7 | |a SRB : Sulforhodamine B |2 nationallicence | |
| 690 | 7 | |a TCA : Trichloro acetic acid |2 nationallicence | |
| 690 | 7 | |a TGF-β1 : Transforming growth factor beta 1 |2 nationallicence | |
| 690 | 7 | |a TIMPs : Tissue inhibitors of MMPs |2 nationallicence | |
| 690 | 7 | |a TMB : 3,3′,5,5′-Tetramethylbenzidine |2 nationallicence | |
| 700 | 1 | |a Brescacin |D L. |u Callerio Foundation Onlus, via A. Fleming 22, 34127, Trieste, Italy |4 aut | |
| 700 | 1 | |a Masi |D A. |u Callerio Foundation Onlus, via A. Fleming 22, 34127, Trieste, Italy |4 aut | |
| 700 | 1 | |a Sava |D G. |u Callerio Foundation Onlus, via A. Fleming 22, 34127, Trieste, Italy |4 aut | |
| 700 | 1 | |a Bergamo |D A. |u Callerio Foundation Onlus, via A. Fleming 22, 34127, Trieste, Italy |4 aut | |
| 773 | 0 | |t JBIC Journal of Biological Inorganic Chemistry |d Springer Berlin Heidelberg |g 20/7(2015-10-01), 1163-1173 |x 0949-8257 |q 20:7<1163 |1 2015 |2 20 |o 775 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00775-015-1297-8 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00775-015-1297-8 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Brescacin |D L. |u Callerio Foundation Onlus, via A. Fleming 22, 34127, Trieste, Italy |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Masi |D A. |u Callerio Foundation Onlus, via A. Fleming 22, 34127, Trieste, Italy |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Sava |D G. |u Callerio Foundation Onlus, via A. Fleming 22, 34127, Trieste, Italy |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Bergamo |D A. |u Callerio Foundation Onlus, via A. Fleming 22, 34127, Trieste, Italy |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t JBIC Journal of Biological Inorganic Chemistry |d Springer Berlin Heidelberg |g 20/7(2015-10-01), 1163-1173 |x 0949-8257 |q 20:7<1163 |1 2015 |2 20 |o 775 | ||