Interaction between lanthanide ions and Saccharomyces cerevisiae cells
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| 008 | 210128e20151001xx s 000 0 eng | ||
| 024 | 7 | 0 | |a 10.1007/s00775-015-1291-1 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00775-015-1291-1 | ||
| 245 | 0 | 0 | |a Interaction between lanthanide ions and Saccharomyces cerevisiae cells |h [Elektronische Daten] |c [Cristian Ene, Lavinia Ruta, Ioana Nicolau, Claudia Popa, Virgil Iordache, Aurora Neagoe, Ileana Farcasanu] |
| 520 | 3 | |a Lanthanides are a group of non-essential elements with important imaging and therapeutic applications. Although trivalent lanthanide ions (Ln3+) are used as potent blockers of Ca2+ channels, the systematic studies correlating Ln3+ accumulation and toxicity to Ca2+ channel blocking activity are scarce. In this study, we made use of the eukaryotic model Saccharomyces cerevisiae to investigate the correlation between Ln3+ accumulation, their toxicity and their capacity to block the exogenous stress-induced Ca2+ influx into the cytosol. It was found that the Ln3+ blocked the Ca2+ entry into the yeast cells only when present at concentration high enough to allow rapid binding to cell surface. At lower concentrations, Ln3+ were taken up by the cell, but Ca2+ blockage was no longer achieved. At 1mM concentration, all ions from the Ln3+ series could block Ca2+ entry into cytosol with the exception of La3+, and to a lesser extent, Pr3+ and Nd3+. The plasma membrane Ca2+-channel Cch1/Mid1 contributed to La3+ and Gd3+ entry into the cells, with a significant preference for La3+. The results open the possibility to obtain cells loaded with controlled amounts and ratios of Ln3+. | |
| 540 | |a SBIC, 2015 | ||
| 690 | 7 | |a Lanthanide |2 nationallicence | |
| 690 | 7 | |a Calcium signaling |2 nationallicence | |
| 690 | 7 | |a Saccharomyces cerevisiae |2 nationallicence | |
| 690 | 7 | |a Aequorin |2 nationallicence | |
| 690 | 7 | |a Alkaline stress |2 nationallicence | |
| 690 | 7 | |a [Ca2+]cyt : Cytosolic Ca2+ |2 nationallicence | |
| 690 | 7 | |a CFU : Colony forming units |2 nationallicence | |
| 690 | 7 | |a EDTA : Ethylenediaminetetraacetic acid |2 nationallicence | |
| 690 | 7 | |a ICP-MS : Inductively coupled plasma-mass spectrometry |2 nationallicence | |
| 690 | 7 | |a Ln3+ : Trivalent lanthanide ions |2 nationallicence | |
| 690 | 7 | |a MES : 2-(N-morpholino)ethanesulfonic acid |2 nationallicence | |
| 690 | 7 | |a MM : Minimal defined medium |2 nationallicence | |
| 690 | 7 | |a RLU : Relative luminescence units |2 nationallicence | |
| 690 | 7 | |a SD-Ura : SD lacking uracil |2 nationallicence | |
| 690 | 7 | |a SE : Standard deviation |2 nationallicence | |
| 690 | 7 | |a WT : Parental "wild-type” strain |2 nationallicence | |
| 690 | 7 | |a YPD : Yeast extract-peptone-dextrose |2 nationallicence | |
| 700 | 1 | |a Ene |D Cristian |u Faculty of Chemistry, University of Bucharest, Sos. Panduri 90-92, Bucharest, Romania |4 aut | |
| 700 | 1 | |a Ruta |D Lavinia |u Faculty of Chemistry, University of Bucharest, Sos. Panduri 90-92, Bucharest, Romania |4 aut | |
| 700 | 1 | |a Nicolau |D Ioana |u Faculty of Chemistry, University of Bucharest, Sos. Panduri 90-92, Bucharest, Romania |4 aut | |
| 700 | 1 | |a Popa |D Claudia |u Faculty of Chemistry, University of Bucharest, Sos. Panduri 90-92, Bucharest, Romania |4 aut | |
| 700 | 1 | |a Iordache |D Virgil |u Faculty of Biology, University of Bucharest, Spl. Independentei 91-95, 050095, Bucharest, Romania |4 aut | |
| 700 | 1 | |a Neagoe |D Aurora |u Faculty of Biology, University of Bucharest, Spl. Independentei 91-95, 050095, Bucharest, Romania |4 aut | |
| 700 | 1 | |a Farcasanu |D Ileana |u Faculty of Chemistry, University of Bucharest, Sos. Panduri 90-92, Bucharest, Romania |4 aut | |
| 773 | 0 | |t JBIC Journal of Biological Inorganic Chemistry |d Springer Berlin Heidelberg |g 20/7(2015-10-01), 1097-1107 |x 0949-8257 |q 20:7<1097 |1 2015 |2 20 |o 775 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00775-015-1291-1 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00775-015-1291-1 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Ene |D Cristian |u Faculty of Chemistry, University of Bucharest, Sos. Panduri 90-92, Bucharest, Romania |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Ruta |D Lavinia |u Faculty of Chemistry, University of Bucharest, Sos. Panduri 90-92, Bucharest, Romania |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Nicolau |D Ioana |u Faculty of Chemistry, University of Bucharest, Sos. Panduri 90-92, Bucharest, Romania |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Popa |D Claudia |u Faculty of Chemistry, University of Bucharest, Sos. Panduri 90-92, Bucharest, Romania |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Iordache |D Virgil |u Faculty of Biology, University of Bucharest, Spl. Independentei 91-95, 050095, Bucharest, Romania |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Neagoe |D Aurora |u Faculty of Biology, University of Bucharest, Spl. Independentei 91-95, 050095, Bucharest, Romania |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Farcasanu |D Ileana |u Faculty of Chemistry, University of Bucharest, Sos. Panduri 90-92, Bucharest, Romania |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t JBIC Journal of Biological Inorganic Chemistry |d Springer Berlin Heidelberg |g 20/7(2015-10-01), 1097-1107 |x 0949-8257 |q 20:7<1097 |1 2015 |2 20 |o 775 | ||