caa a22 4500 60550718X CHVBK 20210128100631.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s00775-015-1291-1 doi (NATIONALLICENCE)springer-10.1007/s00775-015-1291-1 Interaction between lanthanide ions and Saccharomyces cerevisiae cells [Elektronische Daten] [Cristian Ene, Lavinia Ruta, Ioana Nicolau, Claudia Popa, Virgil Iordache, Aurora Neagoe, Ileana Farcasanu] Lanthanides are a group of non-essential elements with important imaging and therapeutic applications. Although trivalent lanthanide ions (Ln3+) are used as potent blockers of Ca2+ channels, the systematic studies correlating Ln3+ accumulation and toxicity to Ca2+ channel blocking activity are scarce. In this study, we made use of the eukaryotic model Saccharomyces cerevisiae to investigate the correlation between Ln3+ accumulation, their toxicity and their capacity to block the exogenous stress-induced Ca2+ influx into the cytosol. It was found that the Ln3+ blocked the Ca2+ entry into the yeast cells only when present at concentration high enough to allow rapid binding to cell surface. At lower concentrations, Ln3+ were taken up by the cell, but Ca2+ blockage was no longer achieved. At 1mM concentration, all ions from the Ln3+ series could block Ca2+ entry into cytosol with the exception of La3+, and to a lesser extent, Pr3+ and Nd3+. The plasma membrane Ca2+-channel Cch1/Mid1 contributed to La3+ and Gd3+ entry into the cells, with a significant preference for La3+. The results open the possibility to obtain cells loaded with controlled amounts and ratios of Ln3+. SBIC, 2015 Lanthanide nationallicence Calcium signaling nationallicence Saccharomyces cerevisiae nationallicence Aequorin nationallicence Alkaline stress nationallicence [Ca2+]cyt : Cytosolic Ca2+ nationallicence CFU : Colony forming units nationallicence EDTA : Ethylenediaminetetraacetic acid nationallicence ICP-MS : Inductively coupled plasma-mass spectrometry nationallicence Ln3+ : Trivalent lanthanide ions nationallicence MES : 2-(N-morpholino)ethanesulfonic acid nationallicence MM : Minimal defined medium nationallicence RLU : Relative luminescence units nationallicence SD-Ura : SD lacking uracil nationallicence SE : Standard deviation nationallicence WT : Parental "wild-type” strain nationallicence YPD : Yeast extract-peptone-dextrose nationallicence Ene Cristian Faculty of Chemistry, University of Bucharest, Sos. Panduri 90-92, Bucharest, Romania aut Ruta Lavinia Faculty of Chemistry, University of Bucharest, Sos. Panduri 90-92, Bucharest, Romania aut Nicolau Ioana Faculty of Chemistry, University of Bucharest, Sos. Panduri 90-92, Bucharest, Romania aut Popa Claudia Faculty of Chemistry, University of Bucharest, Sos. Panduri 90-92, Bucharest, Romania aut Iordache Virgil Faculty of Biology, University of Bucharest, Spl. Independentei 91-95, 050095, Bucharest, Romania aut Neagoe Aurora Faculty of Biology, University of Bucharest, Spl. Independentei 91-95, 050095, Bucharest, Romania aut Farcasanu Ileana Faculty of Chemistry, University of Bucharest, Sos. Panduri 90-92, Bucharest, Romania aut JBIC Journal of Biological Inorganic Chemistry Springer Berlin Heidelberg 20/7(2015-10-01), 1097-1107 0949-8257 20:7<1097 2015 20 775 https://doi.org/10.1007/s00775-015-1291-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00775-015-1291-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ene Cristian Faculty of Chemistry, University of Bucharest, Sos. Panduri 90-92, Bucharest, Romania aut NATIONALLICENCE 700 1- Ruta Lavinia Faculty of Chemistry, University of Bucharest, Sos. Panduri 90-92, Bucharest, Romania aut NATIONALLICENCE 700 1- Nicolau Ioana Faculty of Chemistry, University of Bucharest, Sos. Panduri 90-92, Bucharest, Romania aut NATIONALLICENCE 700 1- Popa Claudia Faculty of Chemistry, University of Bucharest, Sos. Panduri 90-92, Bucharest, Romania aut NATIONALLICENCE 700 1- Iordache Virgil Faculty of Biology, University of Bucharest, Spl. Independentei 91-95, 050095, Bucharest, Romania aut NATIONALLICENCE 700 1- Neagoe Aurora Faculty of Biology, University of Bucharest, Spl. Independentei 91-95, 050095, Bucharest, Romania aut NATIONALLICENCE 700 1- Farcasanu Ileana Faculty of Chemistry, University of Bucharest, Sos. Panduri 90-92, Bucharest, Romania aut NATIONALLICENCE 773 0- JBIC Journal of Biological Inorganic Chemistry Springer Berlin Heidelberg 20/7(2015-10-01), 1097-1107 0949-8257 20:7<1097 2015 20 775