caa a22 4500 605507295 CHVBK 20210128100631.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s00775-014-1226-2 doi (NATIONALLICENCE)springer-10.1007/s00775-014-1226-2 Rhenium and technetium tricarbonyl, {M(CO)3}+ (M=Tc, Re), binding to mammalian metallothioneins: new insights into chemical and radiopharmaceutical implications [Elektronische Daten] [Joan Lecina, Òscar Palacios, Sílvia Atrian, Mercè Capdevila, Joan Suades] This paper deals with the binding of the four mammalian metallothioneins (MTs) to the organometallic metal fragment {fac-M(CO)3}+ (M=99Tc, Re), which is highly promising for the preparation of second-generation radiopharmaceuticals. The study of the transmetallation reaction between zinc and rhenium in Zn7-MT1 by means of UV-vis and CD spectroscopy demonstrated the incorporation of the {fac-Re(CO)3}+ fragment to the MTs. This reaction should be performed at 70°C to accelerate the reaction rate, a result that is consistent with the reported reactivity of the rhenium fragment. ESI-TOF MS demonstrated the formation of mixed-metal species as Zn6,{Re(CO)3}-MT, Zn6,{Re(CO)3}2-MT, and Zn5,{Re(CO)3}3-MT, as well as the different reactivity of the four MT isoforms. Hence, Zn-MT3 showed the highest reactivity, in agreement with its high Cu-thionein character, whereas Zn-MT2 exhibited the lowest reactivity, in line with its high Zn-thionein character. The reactivity of the Zn-loaded forms of MT1 and MT4 is intermediate between those of MT3 and MT2. The study of the binding of the {fac-99Tc(CO)3}+ fragment to MTs showed a significant and very interesting different reactivity in relation to rhenium. The transmetallation reaction is much more effective with technetium than with rhenium and significant amounts of mixed Zn x ,{99Tc(CO)3} y -MT species were formed with the four MT isoforms whereas only MT3 rendered similar amounts of rhenium derivatives. The results obtained in this study support the possible use of technetium for labelling mammalian metallothioneins and also for possible radiopharmaceutical applications. Graphical Abstract: SBIC, 2014 Rhenium nationallicence Technetium nationallicence Metallothionein nationallicence Metal carbonyl nationallicence Mass spectrometry nationallicence Lecina Joan Departament de Química, Facultat de Ciències, Universitat Autònoma de Barcelona, 08193, Cerdanyola del Vallès, Barcelona, Spain aut Palacios Òscar Departament de Química, Facultat de Ciències, Universitat Autònoma de Barcelona, 08193, Cerdanyola del Vallès, Barcelona, Spain aut Atrian Sílvia Departament de Genètica, Facultat de Biologia, Universitat de Barcelona, Av. Diagonal 645, 08028, Barcelona, Spain aut Capdevila Mercè Departament de Química, Facultat de Ciències, Universitat Autònoma de Barcelona, 08193, Cerdanyola del Vallès, Barcelona, Spain aut Suades Joan Departament de Química, Facultat de Ciències, Universitat Autònoma de Barcelona, 08193, Cerdanyola del Vallès, Barcelona, Spain aut JBIC Journal of Biological Inorganic Chemistry Springer Berlin Heidelberg 20/3(2015-04-01), 465-474 0949-8257 20:3<465 2015 20 775 https://doi.org/10.1007/s00775-014-1226-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00775-014-1226-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lecina Joan Departament de Química, Facultat de Ciències, Universitat Autònoma de Barcelona, 08193, Cerdanyola del Vallès, Barcelona, Spain aut NATIONALLICENCE 700 1- Palacios Òscar Departament de Química, Facultat de Ciències, Universitat Autònoma de Barcelona, 08193, Cerdanyola del Vallès, Barcelona, Spain aut NATIONALLICENCE 700 1- Atrian Sílvia Departament de Genètica, Facultat de Biologia, Universitat de Barcelona, Av. Diagonal 645, 08028, Barcelona, Spain aut NATIONALLICENCE 700 1- Capdevila Mercè Departament de Química, Facultat de Ciències, Universitat Autònoma de Barcelona, 08193, Cerdanyola del Vallès, Barcelona, Spain aut NATIONALLICENCE 700 1- Suades Joan Departament de Química, Facultat de Ciències, Universitat Autònoma de Barcelona, 08193, Cerdanyola del Vallès, Barcelona, Spain aut NATIONALLICENCE 773 0- JBIC Journal of Biological Inorganic Chemistry Springer Berlin Heidelberg 20/3(2015-04-01), 465-474 0949-8257 20:3<465 2015 20 775