caa a22 4500 605507325 CHVBK 20210128100631.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s00775-014-1231-5 doi (NATIONALLICENCE)springer-10.1007/s00775-014-1231-5 Incorporation of uranium into a biomimetic apatite: physicochemical and biological aspects [Elektronische Daten] [Grégory Chatelain, Damien Bourgeois, Johann Ravaux, Olivier Averseng, Claude Vidaud, Daniel Meyer] Bone is the main target organ for the storage of several toxic metals, including uranium. But the mode of action of uranium on bones remains poorly understood. To better assess the impact of uranium on bone cells, synthetic biomimetic apatites encompassing a controlled amount of uranium were prepared and analyzed. This study revealed the physicochemical impact of uranium on apatite mineralization: the presence of the metal induces a loss of crystallinity and a lower mineralization rate. The prepared samples were then used as substrates for bone cell culture. Osteoblasts were not sensitive to the presence of uranium in the support, whereas previous results showed a deleterious effect of uranium introduced into a cell culture solution. This work should therefore have some original prospects within the context of toxicological studies concerning the effect of metallic cations on bone cell systems. SBIC, 2014 Apatite nationallicence Biomimetic material nationallicence Bone cells nationallicence Metal ion toxicity nationallicence Uranium nationallicence ACC : Apatite-collagen complex nationallicence FT-IR : Fourrier transform Infrared spectroscopy nationallicence ICP-AES : Inductively coupled plasma atomic emission spectroscopy nationallicence SEM : Scanning electron microscopy nationallicence STD : Standard mineralization of collagen nationallicence XRD : X-ray diffraction nationallicence Chatelain Grégory ICSM, LCPA, BP 17171, 30207, Bagnols-Sur-Cèze, France aut Bourgeois Damien ICSM, LCPA, BP 17171, 30207, Bagnols-Sur-Cèze, France aut Ravaux Johann ICSM, LM2E, BP 17171, 30207, Bagnols-Sur-Cèze, France aut Averseng Olivier CEA, IBEB, SBTN, BP 17171, 30207, Bagnols-Sur-Cèze, France aut Vidaud Claude CEA, IBEB, SBTN, BP 17171, 30207, Bagnols-Sur-Cèze, France aut Meyer Daniel ICSM, LCPA, BP 17171, 30207, Bagnols-Sur-Cèze, France aut JBIC Journal of Biological Inorganic Chemistry Springer Berlin Heidelberg 20/3(2015-04-01), 497-507 0949-8257 20:3<497 2015 20 775 https://doi.org/10.1007/s00775-014-1231-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00775-014-1231-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chatelain Grégory ICSM, LCPA, BP 17171, 30207, Bagnols-Sur-Cèze, France aut NATIONALLICENCE 700 1- Bourgeois Damien ICSM, LCPA, BP 17171, 30207, Bagnols-Sur-Cèze, France aut NATIONALLICENCE 700 1- Ravaux Johann ICSM, LM2E, BP 17171, 30207, Bagnols-Sur-Cèze, France aut NATIONALLICENCE 700 1- Averseng Olivier CEA, IBEB, SBTN, BP 17171, 30207, Bagnols-Sur-Cèze, France aut NATIONALLICENCE 700 1- Vidaud Claude CEA, IBEB, SBTN, BP 17171, 30207, Bagnols-Sur-Cèze, France aut NATIONALLICENCE 700 1- Meyer Daniel ICSM, LCPA, BP 17171, 30207, Bagnols-Sur-Cèze, France aut NATIONALLICENCE 773 0- JBIC Journal of Biological Inorganic Chemistry Springer Berlin Heidelberg 20/3(2015-04-01), 497-507 0949-8257 20:3<497 2015 20 775