caa a22 4500 605507465 CHVBK 20210128100632.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s00775-015-1253-7 doi (NATIONALLICENCE)springer-10.1007/s00775-015-1253-7 Conformational control of the binding of diatomic gases to cytochrome c′ [Elektronische Daten] [Andreea Manole, Demet Kekilli, Dimitri Svistunenko, Michael Wilson, Paul Dobbin, Michael Hough] The cytochromes c′ (CYTcp) are found in denitrifying, methanotrophic and photosynthetic bacteria. These proteins are able to form stable adducts with CO and NO but not with O2. The binding of NO to CYTcp currently provides the best structural model for the NO activation mechanism of soluble guanylate cyclase. Ligand binding in CYTcps has been shown to be highly dependent on residues in both the proximal and distal heme pockets. Group 1 CYTcps typically have a phenylalanine residue positioned close to the distal face of heme, while for group 2, this residue is typically leucine. We have structurally, spectroscopically and kinetically characterised the CYTcp from Shewanella frigidimarina (SFCP), a protein that has a distal phenylalanine residue and a lysine in the proximal pocket in place of the more common arginine. Each monomer of the SFCP dimer folds as a 4-alpha-helical bundle in a similar manner to CYTcps previously characterised. SFCP exhibits biphasic binding kinetics for both NO and CO as a result of the high level of steric hindrance from the aromatic side chain of residue Phe 16. The binding of distal ligands is thus controlled by the conformation of the phenylalanine ring. Only a proximal 5-coordinate NO adduct, confirmed by structural data, is observed with no detectable hexacoordinate distal NO adduct. SBIC, 2015 Biphasic kinetics nationallicence Conformational control nationallicence Proximal nitric oxide nationallicence Gas sensor nationallicence Ligand discrimination nationallicence AXCP : Alcaligenes xylosoxidans cytochrome c′ nationallicence CVCP : Chromatium vinosum cytochrome c′ nationallicence CYTcp : Cytochrome c′ nationallicence RCCP : Rhodobacter capsulatus cytochrome c′ nationallicence RGCP : Rubrivivax gelatinosus cytochrome c′ nationallicence RSCP : Rhodobacter sphaeroides cytochrome c′ nationallicence SFCP : Shewanella frigidimarina cytochrome c′ nationallicence Manole Andreea School of Biological Sciences, University of Essex, Wivenhoe Park, CO4 3SQ, Colchester, Essex, UK aut Kekilli Demet School of Biological Sciences, University of Essex, Wivenhoe Park, CO4 3SQ, Colchester, Essex, UK aut Svistunenko Dimitri School of Biological Sciences, University of Essex, Wivenhoe Park, CO4 3SQ, Colchester, Essex, UK aut Wilson Michael School of Biological Sciences, University of Essex, Wivenhoe Park, CO4 3SQ, Colchester, Essex, UK aut Dobbin Paul School of Biological Sciences, University of Essex, Wivenhoe Park, CO4 3SQ, Colchester, Essex, UK aut Hough Michael School of Biological Sciences, University of Essex, Wivenhoe Park, CO4 3SQ, Colchester, Essex, UK aut JBIC Journal of Biological Inorganic Chemistry Springer Berlin Heidelberg 20/4(2015-06-01), 675-686 0949-8257 20:4<675 2015 20 775 https://doi.org/10.1007/s00775-015-1253-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00775-015-1253-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Manole Andreea School of Biological Sciences, University of Essex, Wivenhoe Park, CO4 3SQ, Colchester, Essex, UK aut NATIONALLICENCE 700 1- Kekilli Demet School of Biological Sciences, University of Essex, Wivenhoe Park, CO4 3SQ, Colchester, Essex, UK aut NATIONALLICENCE 700 1- Svistunenko Dimitri School of Biological Sciences, University of Essex, Wivenhoe Park, CO4 3SQ, Colchester, Essex, UK aut NATIONALLICENCE 700 1- Wilson Michael School of Biological Sciences, University of Essex, Wivenhoe Park, CO4 3SQ, Colchester, Essex, UK aut NATIONALLICENCE 700 1- Dobbin Paul School of Biological Sciences, University of Essex, Wivenhoe Park, CO4 3SQ, Colchester, Essex, UK aut NATIONALLICENCE 700 1- Hough Michael School of Biological Sciences, University of Essex, Wivenhoe Park, CO4 3SQ, Colchester, Essex, UK aut NATIONALLICENCE 773 0- JBIC Journal of Biological Inorganic Chemistry Springer Berlin Heidelberg 20/4(2015-06-01), 675-686 0949-8257 20:4<675 2015 20 775