Structural characterization of metal binding to a cold-adapted frataxin
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| 005 | 20210128100632.0 | ||
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| 008 | 210128e20150601xx s 000 0 eng | ||
| 024 | 7 | 0 | |a 10.1007/s00775-015-1251-9 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00775-015-1251-9 | ||
| 245 | 0 | 0 | |a Structural characterization of metal binding to a cold-adapted frataxin |h [Elektronische Daten] |c [Martín Noguera, Ernesto Roman, Juan Rigal, Alexandra Cousido-Siah, André Mitschler, Alberto Podjarny, Javier Santos] |
| 520 | 3 | |a Frataxin is an evolutionary conserved protein that participates in iron metabolism. Deficiency of this small protein in humans causes a severe neurodegenerative disease known as Friedreich's ataxia. A number of studies indicate that frataxin binds iron and regulates Fe-S cluster biosynthesis. Previous structural studies showed that metal binding occurs mainly in a region of high density of negative charge. However, a comprehensive characterization of the binding sites is required to gain further insights into the mechanistic details of frataxin function. In this work, we have solved the X-ray crystal structures of a cold-adapted frataxin from a psychrophilic bacterium in the presence of cobalt or europium ions. We have identified a number of metal-binding sites, mainly solvent exposed, several of which had not been observed in previous studies on mesophilic homologues. No major structural changes were detected upon metal binding, although the structures exhibit significant changes in crystallographic B-factors. The analysis of these B-factors, in combination with crystal packing and RMSD among structures, suggests the existence of localized changes in the internal motions. Based on these results, we propose that bacterial frataxins possess binding sites of moderate affinity for a quick capture and transfer of iron to other proteins and for the regulation of Fe-S cluster biosynthesis, modulating interactions with partner proteins. | |
| 540 | |a SBIC, 2015 | ||
| 690 | 7 | |a CyaY protein family |2 nationallicence | |
| 690 | 7 | |a Iron binding |2 nationallicence | |
| 690 | 7 | |a X-ray diffraction |2 nationallicence | |
| 690 | 7 | |a Conformational dynamics |2 nationallicence | |
| 690 | 7 | |a Extremophile |2 nationallicence | |
| 700 | 1 | |a Noguera |D Martín |u Instituto de Química y Físico-Química Biológicas, Universidad de Buenos Aires, Junín 956, 1113AAD, Buenos Aires, Argentina |4 aut | |
| 700 | 1 | |a Roman |D Ernesto |u Instituto de Química y Físico-Química Biológicas, Universidad de Buenos Aires, Junín 956, 1113AAD, Buenos Aires, Argentina |4 aut | |
| 700 | 1 | |a Rigal |D Juan |u Instituto de Química y Físico-Química Biológicas, Universidad de Buenos Aires, Junín 956, 1113AAD, Buenos Aires, Argentina |4 aut | |
| 700 | 1 | |a Cousido-Siah |D Alexandra |u Department of Integrative Biology, IGBMC, CNRS, INSERM, Université de Strasbourg, Illkirch, France |4 aut | |
| 700 | 1 | |a Mitschler |D André |u Department of Integrative Biology, IGBMC, CNRS, INSERM, Université de Strasbourg, Illkirch, France |4 aut | |
| 700 | 1 | |a Podjarny |D Alberto |u Department of Integrative Biology, IGBMC, CNRS, INSERM, Université de Strasbourg, Illkirch, France |4 aut | |
| 700 | 1 | |a Santos |D Javier |u Instituto de Química y Físico-Química Biológicas, Universidad de Buenos Aires, Junín 956, 1113AAD, Buenos Aires, Argentina |4 aut | |
| 773 | 0 | |t JBIC Journal of Biological Inorganic Chemistry |d Springer Berlin Heidelberg |g 20/4(2015-06-01), 653-664 |x 0949-8257 |q 20:4<653 |1 2015 |2 20 |o 775 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00775-015-1251-9 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00775-015-1251-9 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Noguera |D Martín |u Instituto de Química y Físico-Química Biológicas, Universidad de Buenos Aires, Junín 956, 1113AAD, Buenos Aires, Argentina |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Roman |D Ernesto |u Instituto de Química y Físico-Química Biológicas, Universidad de Buenos Aires, Junín 956, 1113AAD, Buenos Aires, Argentina |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Rigal |D Juan |u Instituto de Química y Físico-Química Biológicas, Universidad de Buenos Aires, Junín 956, 1113AAD, Buenos Aires, Argentina |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Cousido-Siah |D Alexandra |u Department of Integrative Biology, IGBMC, CNRS, INSERM, Université de Strasbourg, Illkirch, France |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Mitschler |D André |u Department of Integrative Biology, IGBMC, CNRS, INSERM, Université de Strasbourg, Illkirch, France |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Podjarny |D Alberto |u Department of Integrative Biology, IGBMC, CNRS, INSERM, Université de Strasbourg, Illkirch, France |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Santos |D Javier |u Instituto de Química y Físico-Química Biológicas, Universidad de Buenos Aires, Junín 956, 1113AAD, Buenos Aires, Argentina |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t JBIC Journal of Biological Inorganic Chemistry |d Springer Berlin Heidelberg |g 20/4(2015-06-01), 653-664 |x 0949-8257 |q 20:4<653 |1 2015 |2 20 |o 775 | ||