Histidine residues are important for preserving the structure and heme binding to the C. elegans HRG-3 heme-trafficking protein
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| 024 | 7 | 0 | |a 10.1007/s00775-015-1304-0 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00775-015-1304-0 | ||
| 245 | 0 | 0 | |a Histidine residues are important for preserving the structure and heme binding to the C. elegans HRG-3 heme-trafficking protein |h [Elektronische Daten] |c [Ortal Marciano, Yoni Moskovitz, Iqbal Hamza, Sharon Ruthstein] |
| 520 | 3 | |a C. elegans is a heme auxotroph that requires environmental heme for sustenance. As such, worms utilize HRG-3, a small heme-trafficking protein, to traffic heme from the intestine to extra-intestinal tissues and embryos. However, how HRG-3 binds and delivers heme remains unknown. In this study, we utilized electron paramagnetic resonance spectroscopy together with site-directed spin labeling, absorption spectroscopy, circular dichroism, and mutagenesis to gain structural and molecular insights into HRG-3. We showed that HRG-3 is a dimer, whereas H9 and H10 are significant residues that preserve a specific conformational state in the HRG-3 dimer. In the absence of H9 and H10, HRG-3 can still bind heme, although with a different affinity. Furthermore, the heme-binding site is closer to the N-termini than to the C-termini. Taken together, our results lay the groundwork for future mechanistic and structural studies of HRG-3 and inter-tissue heme trafficking in metazoans. | |
| 540 | |a SBIC, 2015 | ||
| 690 | 7 | |a HRG-3 |2 nationallicence | |
| 690 | 7 | |a Heme transfer |2 nationallicence | |
| 690 | 7 | |a EPR |2 nationallicence | |
| 690 | 7 | |a Site-directed spin labeling |2 nationallicence | |
| 690 | 7 | |a Absorption spectroscopy |2 nationallicence | |
| 690 | 7 | |a CD : Circular dichroism |2 nationallicence | |
| 690 | 7 | |a CW : Continuous wave |2 nationallicence | |
| 690 | 7 | |a EPR : Electron paramagnetic resonance |2 nationallicence | |
| 690 | 7 | |a MTSSL : 1-Oxyl-2,2,5,5-tetramethyl-2,5-pyrroline-3-methyl) methanesulfonothioate |2 nationallicence | |
| 690 | 7 | |a SDSL : Site-directed spin labeling |2 nationallicence | |
| 700 | 1 | |a Marciano |D Ortal |u Department of Chemistry, Faculty of Exact Sciences, Bar Ilan University, 5290002, Ramat-Gan, Israel |4 aut | |
| 700 | 1 | |a Moskovitz |D Yoni |u Department of Chemistry, Faculty of Exact Sciences, Bar Ilan University, 5290002, Ramat-Gan, Israel |4 aut | |
| 700 | 1 | |a Hamza |D Iqbal |u Department of Animal and Avian Sciences, University of Maryland, 20742, College Park, MD, USA |4 aut | |
| 700 | 1 | |a Ruthstein |D Sharon |u Department of Chemistry, Faculty of Exact Sciences, Bar Ilan University, 5290002, Ramat-Gan, Israel |4 aut | |
| 773 | 0 | |t JBIC Journal of Biological Inorganic Chemistry |d Springer Berlin Heidelberg |g 20/8(2015-12-01), 1253-1261 |x 0949-8257 |q 20:8<1253 |1 2015 |2 20 |o 775 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00775-015-1304-0 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00775-015-1304-0 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Marciano |D Ortal |u Department of Chemistry, Faculty of Exact Sciences, Bar Ilan University, 5290002, Ramat-Gan, Israel |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Moskovitz |D Yoni |u Department of Chemistry, Faculty of Exact Sciences, Bar Ilan University, 5290002, Ramat-Gan, Israel |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Hamza |D Iqbal |u Department of Animal and Avian Sciences, University of Maryland, 20742, College Park, MD, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Ruthstein |D Sharon |u Department of Chemistry, Faculty of Exact Sciences, Bar Ilan University, 5290002, Ramat-Gan, Israel |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t JBIC Journal of Biological Inorganic Chemistry |d Springer Berlin Heidelberg |g 20/8(2015-12-01), 1253-1261 |x 0949-8257 |q 20:8<1253 |1 2015 |2 20 |o 775 | ||