caa a22 4500 605507589 CHVBK 20210128100632.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s00775-015-1306-y doi (NATIONALLICENCE)springer-10.1007/s00775-015-1306-y Elemental changes in the hippocampal formation following two different formulas of ketogenic diet: an X-ray fluorescence microscopy study [Elektronische Daten] [J. Chwiej, A. Patulska, A. Skoczen, K. Janeczko, M. Ciarach, R. Simon, Z. Setkowicz] The main purpose of the following study was the determination of elemental changes occurring within hippocampal formation as a result of high-fat and carbohydrate-restricted ketogenic diet (KD). To realize it, X-ray fluorescence microscopy was applied for topographic and quantitative analysis of P, S, K, Ca, Fe, Cu, Zn and Se in hippocampal formations taken from rats fed with two different KDs and naive controls. The detailed comparisons were done for sectors 1 and 3 of the Ammon's, the dentate gyrus and hilus of dentate gyrus. The results of elemental analysis showed that the KDs induced statistically significant changes in the accumulation of P, K, Ca, Zn and Se in particular areas of hippocampal formation and these alterations strongly depended on the composition of the diets. Much greater influence on the hippocampal areal densities of examined elements was found for the KD which was characterized by a lower content of carbohydrates, higher content of fats and increased proportion of unsaturated fatty acids. The levels of P, K and Zn decreased whilst those of Ca and Se increased as a result of the treatment with the KDs. The Author(s), 2015 Ketogenic diet nationallicence Hippocampal formation nationallicence Topographic and quantitative elemental analysis nationallicence X-ray fluorescence microscopy nationallicence Synchrotron radiation nationallicence Chwiej J. Faculty of Physics and Applied Computer Science, AGH University of Science and Technology, Krakow, Poland aut Patulska A. Faculty of Physics and Applied Computer Science, AGH University of Science and Technology, Krakow, Poland aut Skoczen A. Faculty of Physics and Applied Computer Science, AGH University of Science and Technology, Krakow, Poland aut Janeczko K. Institute of Zoology, Jagiellonian University, Krakow, Poland aut Ciarach M. Institute of Zoology, Jagiellonian University, Krakow, Poland aut Simon R. ANKA Synchrotron Radiation Facility, Karlsruhe Institute of Technology, Karlsruhe, Germany aut Setkowicz Z. Institute of Zoology, Jagiellonian University, Krakow, Poland aut JBIC Journal of Biological Inorganic Chemistry Springer Berlin Heidelberg 20/8(2015-12-01), 1277-1286 0949-8257 20:8<1277 2015 20 775 https://doi.org/10.1007/s00775-015-1306-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00775-015-1306-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chwiej J. Faculty of Physics and Applied Computer Science, AGH University of Science and Technology, Krakow, Poland aut NATIONALLICENCE 700 1- Patulska A. Faculty of Physics and Applied Computer Science, AGH University of Science and Technology, Krakow, Poland aut NATIONALLICENCE 700 1- Skoczen A. Faculty of Physics and Applied Computer Science, AGH University of Science and Technology, Krakow, Poland aut NATIONALLICENCE 700 1- Janeczko K. Institute of Zoology, Jagiellonian University, Krakow, Poland aut NATIONALLICENCE 700 1- Ciarach M. Institute of Zoology, Jagiellonian University, Krakow, Poland aut NATIONALLICENCE 700 1- Simon R. ANKA Synchrotron Radiation Facility, Karlsruhe Institute of Technology, Karlsruhe, Germany aut NATIONALLICENCE 700 1- Setkowicz Z. Institute of Zoology, Jagiellonian University, Krakow, Poland aut NATIONALLICENCE 773 0- JBIC Journal of Biological Inorganic Chemistry Springer Berlin Heidelberg 20/8(2015-12-01), 1277-1286 0949-8257 20:8<1277 2015 20 775