caa a22 4500 605507597 CHVBK 20210128100632.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s00775-015-1310-2 doi (NATIONALLICENCE)springer-10.1007/s00775-015-1310-2 In vitro evaluation of anticancer and antibacterial activities of cobalt oxide nanoparticles [Elektronische Daten] [Shahanavaj Khan, Anees Ansari, Abdul Khan, Rehan Ahmad, Omar Al-Obaid, Wael Al-Kattan] Cobalt oxide nanoparticles (Co3O4-NPs) were synthesized using simple urea-based thermal decomposition method. Phase purity and particle size of as-synthesized nanoparticles were characterized through X-ray diffraction pattern (XRD) and transmission electron microscopy. Through XRD morphology of the Co3O4-NPs was found to be variable in size with range of 36nm. In our present study, we explored the potential cytotoxic and antibacterial effects of Co3O4-NPs in human colorectal types of cancerous cells (HT29 and SW620) and also nine Gram-positive and Gram-negative bacteria. Co3O4-NPs showed promising anticancer activity against HT29 and SW620 cells with IC50 value of 2.26 and 394.5μg/mL, respectively. However, no significant effect of Co3O4-NPs was observed against bacterial strains. Furthermore, a detailed study has been carried out to investigate the possible mechanism of cell death in HT29 cancer cell line through the analysis of expression level of anti-apoptotic Bcl2 and BclxL markers. Western blot analysis results suggested significant role of Co3O4-NPs exposure in cell death due to apoptosis. Graphical Abstract: SBIC, 2015 Cobalt oxide nanoparticles nationallicence MTT assay nationallicence Cytotoxicity nationallicence Western blot analysis nationallicence Khan Shahanavaj Nanomedicine and Biotechnology Research Unit, Department of Pharmaceutics, College of Pharmacy, King Saud University, PO Box 2457, 11451, Riyadh, Saudi Arabia aut Ansari Anees King Abdullah Institute for Nanotechnology, King Saud University, P.O. Box 2455, 11451, Riyadh, Saudi Arabia aut Khan Abdul Nanomedicine and Biotechnology Research Unit, Department of Pharmaceutics, College of Pharmacy, King Saud University, PO Box 2457, 11451, Riyadh, Saudi Arabia aut Ahmad Rehan Colorectal Research Center, College of Medicine, King Saud University, 11451, Riyadh, Saudi Arabia aut Al-Obaid Omar Colorectal Research Center, College of Medicine, King Saud University, 11451, Riyadh, Saudi Arabia aut Al-Kattan Wael Department of Surgery, Al-Faisal University Riyadh, 11451, Riyadh, Saudi Arabia aut JBIC Journal of Biological Inorganic Chemistry Springer Berlin Heidelberg 20/8(2015-12-01), 1319-1326 0949-8257 20:8<1319 2015 20 775 https://doi.org/10.1007/s00775-015-1310-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00775-015-1310-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Khan Shahanavaj Nanomedicine and Biotechnology Research Unit, Department of Pharmaceutics, College of Pharmacy, King Saud University, PO Box 2457, 11451, Riyadh, Saudi Arabia aut NATIONALLICENCE 700 1- Ansari Anees King Abdullah Institute for Nanotechnology, King Saud University, P.O. Box 2455, 11451, Riyadh, Saudi Arabia aut NATIONALLICENCE 700 1- Khan Abdul Nanomedicine and Biotechnology Research Unit, Department of Pharmaceutics, College of Pharmacy, King Saud University, PO Box 2457, 11451, Riyadh, Saudi Arabia aut NATIONALLICENCE 700 1- Ahmad Rehan Colorectal Research Center, College of Medicine, King Saud University, 11451, Riyadh, Saudi Arabia aut NATIONALLICENCE 700 1- Al-Obaid Omar Colorectal Research Center, College of Medicine, King Saud University, 11451, Riyadh, Saudi Arabia aut NATIONALLICENCE 700 1- Al-Kattan Wael Department of Surgery, Al-Faisal University Riyadh, 11451, Riyadh, Saudi Arabia aut NATIONALLICENCE 773 0- JBIC Journal of Biological Inorganic Chemistry Springer Berlin Heidelberg 20/8(2015-12-01), 1319-1326 0949-8257 20:8<1319 2015 20 775