An effective method for profiling the selenium-binding proteins using its reactive metabolic intermediate
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| 008 | 210128e20150701xx s 000 0 eng | ||
| 024 | 7 | 0 | |a 10.1007/s00775-015-1265-3 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00775-015-1265-3 | ||
| 245 | 0 | 3 | |a An effective method for profiling the selenium-binding proteins using its reactive metabolic intermediate |h [Elektronische Daten] |c [Eriko Hori, Sakura Yoshida, Mamoru Haratake, Sakiko Ura, Takeshi Fuchigami, Morio Nakayama] |
| 520 | 3 | |a Currently, the intracellular reduction and/or transport of selenium still remain unknown. Certain reduced forms of selenium species are thought to be reactive with various endogenous molecules, particularly thiol-containing proteins. In this study, a profiling method for identifying the selenium-binding proteins using l-penicillamine selenotrisulfide (PenSSeSPen) as a model of the selenium metabolic intermediate was applied to the cell lysate generated from the rat liver. Several proteins with cysteine thiol were found to be reactive with PenSSeSPen through the thiol-exchange reaction by MALDI TOF-MS analysis. The most distinctive cysteine-containing protein at m/z 14,313 in the liver cell lysate was identified as the liver fatty acid-binding protein based on a rat protein database search and a tryptic fragmentation experiment. This methodology could be used for determining the selenium-binding proteins and/or selenium-interactive species and provide a better understanding of the selenium metabolism and utilization in biological systems. | |
| 540 | |a SBIC, 2015 | ||
| 690 | 7 | |a Liver fatty acid-binding protein |2 nationallicence | |
| 690 | 7 | |a Mass spectrometry |2 nationallicence | |
| 690 | 7 | |a Selenium |2 nationallicence | |
| 690 | 7 | |a Selenotrisulfide |2 nationallicence | |
| 690 | 7 | |a Thiol exchange |2 nationallicence | |
| 690 | 7 | |a Cys : l-Cysteine |2 nationallicence | |
| 690 | 7 | |a DAN : 2,3-Diaminonaphthalene |2 nationallicence | |
| 690 | 7 | |a DTNB : 5,5′-Dithiobis(2-nitrobenzoic acid) |2 nationallicence | |
| 690 | 7 | |a GPx : Glutathione peroxidase |2 nationallicence | |
| 690 | 7 | |a GSH : Glutathione in the reduced form |2 nationallicence | |
| 690 | 7 | |a LFABP : Liver fatty acid-binding protein |2 nationallicence | |
| 690 | 7 | |a MALDI TOF-MS : Matrix-assisted laser desorption ionization time of flight-mass spectrometry |2 nationallicence | |
| 690 | 7 | |a NEM : N-Ethylmaleimide |2 nationallicence | |
| 690 | 7 | |a Pen : L-Penicillamine |2 nationallicence | |
| 690 | 7 | |a PenSSeSPen : L-Penicillamine selenotrisulfide |2 nationallicence | |
| 690 | 7 | |a SeCys, Sec and U : l-Selenocysteine |2 nationallicence | |
| 690 | 7 | |a STS : Selenotrisulfide |2 nationallicence | |
| 700 | 1 | |a Hori |D Eriko |u Graduate School of Biomedical Sciences, Nagasaki University, 1-14 Bunkyo-machi, 852-8521, Nagasaki, Japan |4 aut | |
| 700 | 1 | |a Yoshida |D Sakura |u Graduate School of Biomedical Sciences, Nagasaki University, 1-14 Bunkyo-machi, 852-8521, Nagasaki, Japan |4 aut | |
| 700 | 1 | |a Haratake |D Mamoru |u Faculty of Pharmaceutical Sciences, Sojo University, 4-22-1 Ikeda, 860-0082, Kumamoto, Japan |4 aut | |
| 700 | 1 | |a Ura |D Sakiko |u Graduate School of Biomedical Sciences, Nagasaki University, 1-14 Bunkyo-machi, 852-8521, Nagasaki, Japan |4 aut | |
| 700 | 1 | |a Fuchigami |D Takeshi |u Graduate School of Biomedical Sciences, Nagasaki University, 1-14 Bunkyo-machi, 852-8521, Nagasaki, Japan |4 aut | |
| 700 | 1 | |a Nakayama |D Morio |u Graduate School of Biomedical Sciences, Nagasaki University, 1-14 Bunkyo-machi, 852-8521, Nagasaki, Japan |4 aut | |
| 773 | 0 | |t JBIC Journal of Biological Inorganic Chemistry |d Springer Berlin Heidelberg |g 20/5(2015-07-01), 781-789 |x 0949-8257 |q 20:5<781 |1 2015 |2 20 |o 775 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00775-015-1265-3 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00775-015-1265-3 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Hori |D Eriko |u Graduate School of Biomedical Sciences, Nagasaki University, 1-14 Bunkyo-machi, 852-8521, Nagasaki, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Yoshida |D Sakura |u Graduate School of Biomedical Sciences, Nagasaki University, 1-14 Bunkyo-machi, 852-8521, Nagasaki, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Haratake |D Mamoru |u Faculty of Pharmaceutical Sciences, Sojo University, 4-22-1 Ikeda, 860-0082, Kumamoto, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Ura |D Sakiko |u Graduate School of Biomedical Sciences, Nagasaki University, 1-14 Bunkyo-machi, 852-8521, Nagasaki, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Fuchigami |D Takeshi |u Graduate School of Biomedical Sciences, Nagasaki University, 1-14 Bunkyo-machi, 852-8521, Nagasaki, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Nakayama |D Morio |u Graduate School of Biomedical Sciences, Nagasaki University, 1-14 Bunkyo-machi, 852-8521, Nagasaki, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t JBIC Journal of Biological Inorganic Chemistry |d Springer Berlin Heidelberg |g 20/5(2015-07-01), 781-789 |x 0949-8257 |q 20:5<781 |1 2015 |2 20 |o 775 | ||