The response of Ω-loop D dynamics to truncation of trimethyllysine 72 of yeast iso-1-cytochrome c depends on the nature of loop deformation
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| 024 | 7 | 0 | |a 10.1007/s00775-015-1267-1 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00775-015-1267-1 | ||
| 245 | 0 | 4 | |a The response of Ω-loop D dynamics to truncation of trimethyllysine 72 of yeast iso-1-cytochrome c depends on the nature of loop deformation |h [Elektronische Daten] |c [Levi McClelland, Sean Seagraves, Md. Khan, Melisa Cherney, Swati Bandi, Justin Culbertson, Bruce Bowler] |
| 520 | 3 | |a Trimethyllysine 72 (tmK72) has been suggested to play a role in sterically constraining the heme crevice dynamics of yeast iso-1-cytochrome c mediated by the Ω-loop D cooperative substructure (residues 70-85). A tmK72A mutation causes a gain in peroxidase activity, a function of cytochrome c that is important early in apoptosis. More than one higher energy state is accessible for the Ω-loop D substructure via tier 0 dynamics. Two of these are alkaline conformers mediated by Lys73 and Lys79. In the current work, the effect of the tmK72A mutation on the thermodynamic and kinetic properties of wild-type iso-1-cytochrome c (yWT versus WT*) and on variants carrying a K73H mutation (yWT/K73H versus WT*/K73H) is studied. Whereas the tmK72A mutation confers increased peroxidase activity in wild-type yeast iso-1-cytochrome c and increased dynamics for formation of a previously studied His79-heme alkaline conformer, the tmK72A mutation speeds return of the His73-heme alkaline conformer to the native state through destabilization of the His73-heme alkaline conformer relative to the native conformer. These opposing behaviors demonstrate that the response of the dynamics of a protein substructure to mutation depends on the nature of the perturbation to the substructure. For a protein substructure which mediates more than one function of a protein through multiple non-native structures, a mutation could change the partitioning between these functions. The current results suggest that the tier 0 dynamics of Ω-loop D that mediates peroxidase activity has similarities to the tier 0 dynamics required to form the His79-heme alkaline conformer. | |
| 540 | |a SBIC, 2015 | ||
| 690 | 7 | |a Cooperative substructure dynamics |2 nationallicence | |
| 690 | 7 | |a Cytochrome c |2 nationallicence | |
| 690 | 7 | |a Alkaline conformational transition |2 nationallicence | |
| 690 | 7 | |a Conformationally gated electron transfer |2 nationallicence | |
| 690 | 7 | |a Apoptosis |2 nationallicence | |
| 690 | 7 | |a ET : Electron transfer |2 nationallicence | |
| 690 | 7 | |a gated ET : Conformationally gated electron transfer |2 nationallicence | |
| 690 | 7 | |a GdnHCl : Guanidine hydrochloride |2 nationallicence | |
| 690 | 7 | |a iso-1-Cyt c : Iso-1-cytochrome c |2 nationallicence | |
| 690 | 7 | |a tmK : Trimethyllysine |2 nationallicence | |
| 700 | 1 | |a McClelland |D Levi |u Department of Chemistry and Biochemistry, Center for Biomolecular Structure and Dynamics, University of Montana, 59812, Missoula, MT, USA |4 aut | |
| 700 | 1 | |a Seagraves |D Sean |u Department of Chemistry and Biochemistry, Center for Biomolecular Structure and Dynamics, University of Montana, 59812, Missoula, MT, USA |4 aut | |
| 700 | 1 | |a Khan |D Md |u Department of Chemistry and Biochemistry, Center for Biomolecular Structure and Dynamics, University of Montana, 59812, Missoula, MT, USA |4 aut | |
| 700 | 1 | |a Cherney |D Melisa |u Department of Chemistry and Biochemistry, Center for Biomolecular Structure and Dynamics, University of Montana, 59812, Missoula, MT, USA |4 aut | |
| 700 | 1 | |a Bandi |D Swati |u Department of Chemistry and Biochemistry, Center for Biomolecular Structure and Dynamics, University of Montana, 59812, Missoula, MT, USA |4 aut | |
| 700 | 1 | |a Culbertson |D Justin |u Department of Chemistry and Biochemistry, Center for Biomolecular Structure and Dynamics, University of Montana, 59812, Missoula, MT, USA |4 aut | |
| 700 | 1 | |a Bowler |D Bruce |u Department of Chemistry and Biochemistry, Center for Biomolecular Structure and Dynamics, University of Montana, 59812, Missoula, MT, USA |4 aut | |
| 773 | 0 | |t JBIC Journal of Biological Inorganic Chemistry |d Springer Berlin Heidelberg |g 20/5(2015-07-01), 805-819 |x 0949-8257 |q 20:5<805 |1 2015 |2 20 |o 775 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00775-015-1267-1 |q text/html |z Onlinezugriff via DOI |
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| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00775-015-1267-1 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a McClelland |D Levi |u Department of Chemistry and Biochemistry, Center for Biomolecular Structure and Dynamics, University of Montana, 59812, Missoula, MT, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Seagraves |D Sean |u Department of Chemistry and Biochemistry, Center for Biomolecular Structure and Dynamics, University of Montana, 59812, Missoula, MT, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Khan |D Md |u Department of Chemistry and Biochemistry, Center for Biomolecular Structure and Dynamics, University of Montana, 59812, Missoula, MT, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Cherney |D Melisa |u Department of Chemistry and Biochemistry, Center for Biomolecular Structure and Dynamics, University of Montana, 59812, Missoula, MT, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Bandi |D Swati |u Department of Chemistry and Biochemistry, Center for Biomolecular Structure and Dynamics, University of Montana, 59812, Missoula, MT, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Culbertson |D Justin |u Department of Chemistry and Biochemistry, Center for Biomolecular Structure and Dynamics, University of Montana, 59812, Missoula, MT, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Bowler |D Bruce |u Department of Chemistry and Biochemistry, Center for Biomolecular Structure and Dynamics, University of Montana, 59812, Missoula, MT, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t JBIC Journal of Biological Inorganic Chemistry |d Springer Berlin Heidelberg |g 20/5(2015-07-01), 805-819 |x 0949-8257 |q 20:5<805 |1 2015 |2 20 |o 775 | ||