Changes in metabolic pathways of Desulfovibrio alaskensis G20 cells induced by molybdate excess
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| 024 | 7 | 0 | |a 10.1007/s00775-014-1224-4 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00775-014-1224-4 | ||
| 245 | 0 | 0 | |a Changes in metabolic pathways of Desulfovibrio alaskensis G20 cells induced by molybdate excess |h [Elektronische Daten] |c [Rashmi Nair, Célia Silveira, Mário Diniz, Maria Almeida, Jose Moura, Maria Rivas] |
| 520 | 3 | |a The activity of sulfate-reducing bacteria (SRB) intensifies the problems associated to corrosion of metals and the solution entails significant economic costs. Although molybdate can be used to control the negative effects of these organisms, the mechanisms triggered in the cells exposed to Mo-excess are poorly understood. In this work, the effects of molybdate ions on the growth and morphology of the SRB Desulfovibrio alaskensis G20 (DaG20) were investigated. In addition, the cellular localization, ion uptake and regulation of protein expression were studied. We found that molybdate concentrations ranging between 50 and 150µM produce a twofold increase in the doubling time with this effect being more significant at 200µM molybdate (five times increase in the doubling time). It was also observed that 500µM molybdate completely inhibits the cellular growth. On the context of protein regulation, we found that several enzymes involved in energy metabolism, cellular division and metal uptake processes were particularly influenced under the conditions tested. An overall description of some of the mechanisms involved in the DaG20 adaptation to molybdate-stress conditions is discussed. | |
| 540 | |a SBIC, 2014 | ||
| 690 | 7 | |a Sulfate-reducing bacteria |2 nationallicence | |
| 690 | 7 | |a Hydrogen cycling |2 nationallicence | |
| 690 | 7 | |a Molybdenum |2 nationallicence | |
| 690 | 7 | |a Tungsten |2 nationallicence | |
| 690 | 7 | |a Microbiologically influenced corrosion |2 nationallicence | |
| 690 | 7 | |a AOR : Aldehyde oxidoreductase |2 nationallicence | |
| 690 | 7 | |a ATP : Adenosine triphosphate |2 nationallicence | |
| 690 | 7 | |a D. : Desulfovibrio |2 nationallicence | |
| 690 | 7 | |a Da G20 : Desulfovibrio alaskensis G20 |2 nationallicence | |
| 690 | 7 | |a DCPIP : 2,6-Dichlorophenol-iodophenol |2 nationallicence | |
| 690 | 7 | |a Dv H : Desulfovibrio vulgaris Hildenborough |2 nationallicence | |
| 690 | 7 | |a EPS : Extracellular polymeric substance |2 nationallicence | |
| 690 | 7 | |a IEF : Isoelectric focusing |2 nationallicence | |
| 690 | 7 | |a ICP-AES : Inductively coupled plasma atomic emission spectroscopy |2 nationallicence | |
| 690 | 7 | |a MALDI-TOF : Matrix-assisted laser desorption ionization time-of-flight |2 nationallicence | |
| 690 | 7 | |a MIC : Microbiologically influenced corrosion |2 nationallicence | |
| 690 | 7 | |a SEM : Scanning electron microscopy |2 nationallicence | |
| 690 | 7 | |a SRB : Sulfate-reducing bacteria |2 nationallicence | |
| 690 | 7 | |a TCA : Tricarboxylic acid cycle |2 nationallicence | |
| 690 | 7 | |a Tris-acetate/HCl : Tris(hydroxymethyl)aminomethane acetate/hydrochloride |2 nationallicence | |
| 690 | 7 | |a 2-DE : Two-dimensional electrophoresis |2 nationallicence | |
| 700 | 1 | |a Nair |D Rashmi |u REQUIMTE/CQFB, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516, Caparica, Portugal |4 aut | |
| 700 | 1 | |a Silveira |D Célia |u REQUIMTE/CQFB, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516, Caparica, Portugal |4 aut | |
| 700 | 1 | |a Diniz |D Mário |u REQUIMTE/CQFB, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516, Caparica, Portugal |4 aut | |
| 700 | 1 | |a Almeida |D Maria |u REQUIMTE/CQFB, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516, Caparica, Portugal |4 aut | |
| 700 | 1 | |a Moura |D Jose |u REQUIMTE/CQFB, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516, Caparica, Portugal |4 aut | |
| 700 | 1 | |a Rivas |D Maria |u REQUIMTE/CQFB, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516, Caparica, Portugal |4 aut | |
| 773 | 0 | |t JBIC Journal of Biological Inorganic Chemistry |d Springer Berlin Heidelberg |g 20/2(2015-03-01), 311-322 |x 0949-8257 |q 20:2<311 |1 2015 |2 20 |o 775 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00775-014-1224-4 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00775-014-1224-4 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Nair |D Rashmi |u REQUIMTE/CQFB, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516, Caparica, Portugal |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Silveira |D Célia |u REQUIMTE/CQFB, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516, Caparica, Portugal |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Diniz |D Mário |u REQUIMTE/CQFB, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516, Caparica, Portugal |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Almeida |D Maria |u REQUIMTE/CQFB, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516, Caparica, Portugal |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Moura |D Jose |u REQUIMTE/CQFB, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516, Caparica, Portugal |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Rivas |D Maria |u REQUIMTE/CQFB, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516, Caparica, Portugal |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t JBIC Journal of Biological Inorganic Chemistry |d Springer Berlin Heidelberg |g 20/2(2015-03-01), 311-322 |x 0949-8257 |q 20:2<311 |1 2015 |2 20 |o 775 | ||