caa a22 4500 605507805 CHVBK 20210128100633.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s00775-014-1224-4 doi (NATIONALLICENCE)springer-10.1007/s00775-014-1224-4 Changes in metabolic pathways of Desulfovibrio alaskensis G20 cells induced by molybdate excess [Elektronische Daten] [Rashmi Nair, Célia Silveira, Mário Diniz, Maria Almeida, Jose Moura, Maria Rivas] The activity of sulfate-reducing bacteria (SRB) intensifies the problems associated to corrosion of metals and the solution entails significant economic costs. Although molybdate can be used to control the negative effects of these organisms, the mechanisms triggered in the cells exposed to Mo-excess are poorly understood. In this work, the effects of molybdate ions on the growth and morphology of the SRB Desulfovibrio alaskensis G20 (DaG20) were investigated. In addition, the cellular localization, ion uptake and regulation of protein expression were studied. We found that molybdate concentrations ranging between 50 and 150µM produce a twofold increase in the doubling time with this effect being more significant at 200µM molybdate (five times increase in the doubling time). It was also observed that 500µM molybdate completely inhibits the cellular growth. On the context of protein regulation, we found that several enzymes involved in energy metabolism, cellular division and metal uptake processes were particularly influenced under the conditions tested. An overall description of some of the mechanisms involved in the DaG20 adaptation to molybdate-stress conditions is discussed. SBIC, 2014 Sulfate-reducing bacteria nationallicence Hydrogen cycling nationallicence Molybdenum nationallicence Tungsten nationallicence Microbiologically influenced corrosion nationallicence AOR : Aldehyde oxidoreductase nationallicence ATP : Adenosine triphosphate nationallicence D. : Desulfovibrio nationallicence Da G20 : Desulfovibrio alaskensis G20 nationallicence DCPIP : 2,6-Dichlorophenol-iodophenol nationallicence Dv H : Desulfovibrio vulgaris Hildenborough nationallicence EPS : Extracellular polymeric substance nationallicence IEF : Isoelectric focusing nationallicence ICP-AES : Inductively coupled plasma atomic emission spectroscopy nationallicence MALDI-TOF : Matrix-assisted laser desorption ionization time-of-flight nationallicence MIC : Microbiologically influenced corrosion nationallicence SEM : Scanning electron microscopy nationallicence SRB : Sulfate-reducing bacteria nationallicence TCA : Tricarboxylic acid cycle nationallicence Tris-acetate/HCl : Tris(hydroxymethyl)aminomethane acetate/hydrochloride nationallicence 2-DE : Two-dimensional electrophoresis nationallicence Nair Rashmi REQUIMTE/CQFB, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516, Caparica, Portugal aut Silveira Célia REQUIMTE/CQFB, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516, Caparica, Portugal aut Diniz Mário REQUIMTE/CQFB, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516, Caparica, Portugal aut Almeida Maria REQUIMTE/CQFB, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516, Caparica, Portugal aut Moura Jose REQUIMTE/CQFB, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516, Caparica, Portugal aut Rivas Maria REQUIMTE/CQFB, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516, Caparica, Portugal aut JBIC Journal of Biological Inorganic Chemistry Springer Berlin Heidelberg 20/2(2015-03-01), 311-322 0949-8257 20:2<311 2015 20 775 https://doi.org/10.1007/s00775-014-1224-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00775-014-1224-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nair Rashmi REQUIMTE/CQFB, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516, Caparica, Portugal aut NATIONALLICENCE 700 1- Silveira Célia REQUIMTE/CQFB, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516, Caparica, Portugal aut NATIONALLICENCE 700 1- Diniz Mário REQUIMTE/CQFB, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516, Caparica, Portugal aut NATIONALLICENCE 700 1- Almeida Maria REQUIMTE/CQFB, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516, Caparica, Portugal aut NATIONALLICENCE 700 1- Moura Jose REQUIMTE/CQFB, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516, Caparica, Portugal aut NATIONALLICENCE 700 1- Rivas Maria REQUIMTE/CQFB, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516, Caparica, Portugal aut NATIONALLICENCE 773 0- JBIC Journal of Biological Inorganic Chemistry Springer Berlin Heidelberg 20/2(2015-03-01), 311-322 0949-8257 20:2<311 2015 20 775