A sensitive and stable amperometric nitrate biosensor employing Arabidopsis thaliana nitrate reductase
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| 024 | 7 | 0 | |a 10.1007/s00775-014-1171-0 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00775-014-1171-0 | ||
| 245 | 0 | 2 | |a A sensitive and stable amperometric nitrate biosensor employing Arabidopsis thaliana nitrate reductase |h [Elektronische Daten] |c [Palraj Kalimuthu, Katrin Fischer-Schrader, Günter Schwarz, Paul Bernhardt] |
| 520 | 3 | |a Nitrate reductase (NR) from the plant Arabidopsis thaliana has been employed in the development of an amperometric nitrate biosensor that functions at physiological pH. The anion anthraquinone-2-sulfonate (AQ) is used as an effective artificial electron transfer partner for NR at a glassy carbon (GC) electrode. Nitrate is enzymatically reduced to nitrite and the oxidized form of NR is electrochemically reduced by the hydroquinone form of the mediator (AQH2). The GC/NR electrode shows a pronounced cathodic wave for nitrate reduction and the catalytic current increases linearly in the nitrate concentration range of 10-400µM with a correlation coefficient of 0.989. Using an amperometric method, a low detection limit of 0.76nM (S/N=3) was achieved. The practical application of the present electrochemical biosensor was demonstrated by the determination of nitrate concentration in natural water samples and the results agreed well with a standard spectroscopic method. Graphical Abstract: | |
| 540 | |a SBIC, 2014 | ||
| 690 | 7 | |a Nitrate reductase |2 nationallicence | |
| 690 | 7 | |a Voltammetry |2 nationallicence | |
| 690 | 7 | |a Molybdenum |2 nationallicence | |
| 700 | 1 | |a Kalimuthu |D Palraj |u School of Chemistry and Molecular Biosciences, University of Queensland, 4072, Brisbane, Australia |4 aut | |
| 700 | 1 | |a Fischer-Schrader |D Katrin |u Department of Chemistry and Center for Molecular Medicine, Institute of Biochemistry, Cologne University, Zülicher Str. 47, 50674, Cologne, Germany |4 aut | |
| 700 | 1 | |a Schwarz |D Günter |u Department of Chemistry and Center for Molecular Medicine, Institute of Biochemistry, Cologne University, Zülicher Str. 47, 50674, Cologne, Germany |4 aut | |
| 700 | 1 | |a Bernhardt |D Paul |u School of Chemistry and Molecular Biosciences, University of Queensland, 4072, Brisbane, Australia |4 aut | |
| 773 | 0 | |t JBIC Journal of Biological Inorganic Chemistry |d Springer Berlin Heidelberg |g 20/2(2015-03-01), 385-393 |x 0949-8257 |q 20:2<385 |1 2015 |2 20 |o 775 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00775-014-1171-0 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00775-014-1171-0 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Kalimuthu |D Palraj |u School of Chemistry and Molecular Biosciences, University of Queensland, 4072, Brisbane, Australia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Fischer-Schrader |D Katrin |u Department of Chemistry and Center for Molecular Medicine, Institute of Biochemistry, Cologne University, Zülicher Str. 47, 50674, Cologne, Germany |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Schwarz |D Günter |u Department of Chemistry and Center for Molecular Medicine, Institute of Biochemistry, Cologne University, Zülicher Str. 47, 50674, Cologne, Germany |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Bernhardt |D Paul |u School of Chemistry and Molecular Biosciences, University of Queensland, 4072, Brisbane, Australia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t JBIC Journal of Biological Inorganic Chemistry |d Springer Berlin Heidelberg |g 20/2(2015-03-01), 385-393 |x 0949-8257 |q 20:2<385 |1 2015 |2 20 |o 775 | ||