caa a22 4500 605509166 CHVBK 20210128100640.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s10452-015-9506-7 doi (NATIONALLICENCE)springer-10.1007/s10452-015-9506-7 The first molecular characterization of the genus Hirudo on the territory of Serbia: estimation of endangerment [Elektronische Daten] [Ivana Živić, Tijana Radosavljević, Katarina Stojanović, Andjeljko Petrović] Several recent morphological studies of the genus Hirudo indicated that only Hirudo verbana is present in Serbia, while Hirudo medicinalis is completely absent. In order to test this hypothesis, specimens of medicinal leech from Serbia are analyzed for the first time by the method of DNA identification. The results show that individuals belonging to the species H. medicinalis are absent on the territory of Serbia, where only specimens of the sister (congener) species H. verbana can be found instead. All of the analyzed specimens can be related to five haplotypes. Two of the haplotypes are previously known from Ukraine, while three are newly discovered. It follows that genetic differentiation into eastern and western clades of the species H. verbana is unrealistic. Much more detailed sampling throughout the entire area is needed in order to establish the precise distribution of haplotypes. Factors posing a threat to both individual populations and the species as a whole are also considered as a basis for undertaking conservation measures for preservation of the species H. verbana. Such measures are necessary in view of the fact that for the past 8years, H. verbana has been exported from Serbia to Western European countries instead of H. medicinalis (which does not live on the territory of Serbia). On the basis of the obtained results, the degree of threat to the species H. verbana is defined and preliminary protective measures are proposed for the conservation of this endangered species of aquatic invertebrate. Springer Science+Business Media Dordrecht, 2015 Hirudo verbana nationallicence DNK identification nationallicence Estimation of endangerment nationallicence Živić Ivana Faculty of Biology, Institute of Zoology, University of Belgrade, Studentski trg 16, 11000, Belgrade, Serbia aut Radosavljević Tijana Faculty of Biology, Institute of Zoology, University of Belgrade, Studentski trg 16, 11000, Belgrade, Serbia aut Stojanović Katarina Faculty of Biology, Institute of Zoology, University of Belgrade, Studentski trg 16, 11000, Belgrade, Serbia aut Petrović Andjeljko Faculty of Biology, Institute of Zoology, University of Belgrade, Studentski trg 16, 11000, Belgrade, Serbia aut Aquatic Ecology Springer Netherlands 49/1(2015-03-01), 81-90 1386-2588 49:1<81 2015 49 10452 https://doi.org/10.1007/s10452-015-9506-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10452-015-9506-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Živić Ivana Faculty of Biology, Institute of Zoology, University of Belgrade, Studentski trg 16, 11000, Belgrade, Serbia aut NATIONALLICENCE 700 1- Radosavljević Tijana Faculty of Biology, Institute of Zoology, University of Belgrade, Studentski trg 16, 11000, Belgrade, Serbia aut NATIONALLICENCE 700 1- Stojanović Katarina Faculty of Biology, Institute of Zoology, University of Belgrade, Studentski trg 16, 11000, Belgrade, Serbia aut NATIONALLICENCE 700 1- Petrović Andjeljko Faculty of Biology, Institute of Zoology, University of Belgrade, Studentski trg 16, 11000, Belgrade, Serbia aut NATIONALLICENCE 773 0- Aquatic Ecology Springer Netherlands 49/1(2015-03-01), 81-90 1386-2588 49:1<81 2015 49 10452