caa a22 4500 605509174 CHVBK 20210128100640.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s10452-015-9523-6 doi (NATIONALLICENCE)springer-10.1007/s10452-015-9523-6 A case study of the past CH4 cycle in lakes by the combined use of dual isotopes (carbon and hydrogen) and ancient DNA of methane-oxidizing bacteria: rearing experiment and application to Lake Remoray (eastern France) [Elektronische Daten] [Simon Belle, Valérie Verneaux, Laurent Millet, Claire Parent, Michel Magny] This study aims at estimating the potential of the hydrogen stable isotope (δ2H) analysis of chironomid remains (HC) to reconstruct past changes in the methane (CH4) cycle in lakes. A rearing experiment was first designed to assess the contribution of hydrogen derived from diet to the chironomid biomass and the offset between larvae and their HC. Results from the rearing experiment show that hydrogen stable isotope applied to the chironomid HC seems to be a powerful geochemical tracer because a large proportion (about 85%) of the total hydrogen in the chironomid biomass is derived from the diet and that the application of a corrected factor to the HC values allows estimating the δ2H of the chironomid larvae. We have then tested the interest of the HC δ2H analysis in addition to other approaches to reconstruct CH4 production and consumption along a sediment core covering the last 1500years and collected in the deepest part of Lake Remoray (Jura Mountains, France). δ13C and δ2H analyses were performed on both HC and sedimentary organic matter and were combined with an analysis of ancient DNA (aDNA) of methane-oxidizing bacteria (MOB). Results show that MOB is an important part of the total bacteria aDNA all along the studied period. Moreover, δ13C data confirm that MOB may be an important source of carbon for the chironomid biomass (between 20 and 54%). Finally, δ2H values appear fastidious to obtain in the paleolimnological context; however, this tracer may provide evidence that CO2 reduction is the main dominant process of methanogenesis in Lake Remoray. δ2H analysis could be useful to distinguish the long-term evolution and the relative contribution of the two pathways of methanogenesis. However, δ2H rearing experiments are needed to better estimate the offset and the contribution values. Springer Science+Business Media Dordrecht, 2015 Chironomidae nationallicence Hydrogen stable isotopes nationallicence Carbon stable isotopes nationallicence Methane-oxidizing bacteria nationallicence Methanogenesis nationallicence Ancient DNA nationallicence Belle Simon Laboratoire de Chrono-Environnement, UMR CNRS 6249, Université de Franche-Comté, Besançon, France aut Verneaux Valérie Laboratoire de Chrono-Environnement, UMR CNRS 6249, Université de Franche-Comté, Besançon, France aut Millet Laurent Laboratoire de Chrono-Environnement, UMR CNRS 6249, Université de Franche-Comté, Besançon, France aut Parent Claire Laboratoire de Chrono-Environnement, UMR CNRS 6249, Université de Franche-Comté, Besançon, France aut Magny Michel Laboratoire de Chrono-Environnement, UMR CNRS 6249, Université de Franche-Comté, Besançon, France aut Aquatic Ecology Springer Netherlands 49/3(2015-09-01), 279-291 1386-2588 49:3<279 2015 49 10452 https://doi.org/10.1007/s10452-015-9523-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10452-015-9523-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Belle Simon Laboratoire de Chrono-Environnement, UMR CNRS 6249, Université de Franche-Comté, Besançon, France aut NATIONALLICENCE 700 1- Verneaux Valérie Laboratoire de Chrono-Environnement, UMR CNRS 6249, Université de Franche-Comté, Besançon, France aut NATIONALLICENCE 700 1- Millet Laurent Laboratoire de Chrono-Environnement, UMR CNRS 6249, Université de Franche-Comté, Besançon, France aut NATIONALLICENCE 700 1- Parent Claire Laboratoire de Chrono-Environnement, UMR CNRS 6249, Université de Franche-Comté, Besançon, France aut NATIONALLICENCE 700 1- Magny Michel Laboratoire de Chrono-Environnement, UMR CNRS 6249, Université de Franche-Comté, Besançon, France aut NATIONALLICENCE 773 0- Aquatic Ecology Springer Netherlands 49/3(2015-09-01), 279-291 1386-2588 49:3<279 2015 49 10452