caa a22 4500 605509271 CHVBK 20210128100640.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s10452-015-9520-9 doi (NATIONALLICENCE)springer-10.1007/s10452-015-9520-9 Identification of species and genotypic compositions of Cryptomonas (Cryptophyceae) populations in the eutrophic Lake Hira, Japan, using single-cell PCR [Elektronische Daten] [Hiroshi Nishino, Yoshikuni Hodoki, Shoji Thottathil, Kako Ohbayashi, Yoshitake Takao, Shin-ichi Nakano] Single-cell PCR and gene sequencing were conducted to evaluate species and genotypic compositions of Cryptomonas in the eutrophic Lake Hira, Japan. We determined the sequences of nuclear internal transcribed spacer 2 region from single Cryptomonas cells with a high success rate (83.3-97.9%), excluding one case (56.3%). A total of 325 sequences were obtained over eight sampling days from May 28, 2012, to October 3, 2012, and phylogenetic analysis indicated that all sequences were divided into six groups. Four groups were clustered together with known sequences of C. curvata, C. marssonii, C. pyrenoidifera or C. tetrapyrenoidosa, although the sequences of the other two groups did not show high similarity to known Cryptomonas species. Cryptomonas curvata dominated during the study period (45-98%), and unidentified Cryptomonas species (group 2) became dominant at high water temperatures. The genotypic composition of C. curvata also varied temporarily, suggesting that the genotypic composition of Cryptomonas was susceptible to environmental changes. These results indicated that single-cell PCR can be used to analyze the species composition and ecology of Cryptomonas. Springer Science+Business Media Dordrecht, 2015 Cryptomonas nationallicence Nuclear internal transcribed spacer 2 (ITS2) nationallicence Single-cell PCR nationallicence Genotypic composition nationallicence Nishino Hiroshi Center for Ecological Research, Kyoto University, Hirano 2-509-3, 520-2113, Otsu, Shiga, Japan aut Hodoki Yoshikuni Department of Biology, Keio University, 4-1-1 Hiyoshi, Kohoku-ku, 223-8521, Yokohama, Kanagawa, Japan aut Thottathil Shoji Center for Ecological Research, Kyoto University, Hirano 2-509-3, 520-2113, Otsu, Shiga, Japan aut Ohbayashi Kako Department of General Systems Studies, University of Tokyo, 3-8-1 Komaba, Meguro-ku, 153-8902, Tokyo, Japan aut Takao Yoshitake Department of Marine Bioscience, Fukui Prefectural University, 1-1 Gakuen-cho, 917-0003, Obama, Fukui, Japan aut Nakano Shin-ichi Center for Ecological Research, Kyoto University, Hirano 2-509-3, 520-2113, Otsu, Shiga, Japan aut Aquatic Ecology Springer Netherlands 49/3(2015-09-01), 263-272 1386-2588 49:3<263 2015 49 10452 https://doi.org/10.1007/s10452-015-9520-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10452-015-9520-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nishino Hiroshi Center for Ecological Research, Kyoto University, Hirano 2-509-3, 520-2113, Otsu, Shiga, Japan aut NATIONALLICENCE 700 1- Hodoki Yoshikuni Department of Biology, Keio University, 4-1-1 Hiyoshi, Kohoku-ku, 223-8521, Yokohama, Kanagawa, Japan aut NATIONALLICENCE 700 1- Thottathil Shoji Center for Ecological Research, Kyoto University, Hirano 2-509-3, 520-2113, Otsu, Shiga, Japan aut NATIONALLICENCE 700 1- Ohbayashi Kako Department of General Systems Studies, University of Tokyo, 3-8-1 Komaba, Meguro-ku, 153-8902, Tokyo, Japan aut NATIONALLICENCE 700 1- Takao Yoshitake Department of Marine Bioscience, Fukui Prefectural University, 1-1 Gakuen-cho, 917-0003, Obama, Fukui, Japan aut NATIONALLICENCE 700 1- Nakano Shin-ichi Center for Ecological Research, Kyoto University, Hirano 2-509-3, 520-2113, Otsu, Shiga, Japan aut NATIONALLICENCE 773 0- Aquatic Ecology Springer Netherlands 49/3(2015-09-01), 263-272 1386-2588 49:3<263 2015 49 10452