An investigation of folic acid-protein association sites and the effect of this association on folic acid self-assembly
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| 008 | 210128e20151201xx s 000 0 eng | ||
| 024 | 7 | 0 | |a 10.1007/s00894-015-2847-2 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00894-015-2847-2 | ||
| 245 | 0 | 3 | |a An investigation of folic acid-protein association sites and the effect of this association on folic acid self-assembly |h [Elektronische Daten] |c [Rajat Gupta, Prasanta Kalita, Omkar Patil, Sanat Mohanty] |
| 520 | 3 | |a The contribution of folic acid (FA)-tryptophan interactions to FA-protein association was investigated in the context of using FA as a drug carrier in protein delivery systems. Bovine serum albumin (BSA) and indolicidin were used as model proteins in the study. The FA-BSA complex was characterized by using the Bradford reagent to identify the impact of FA-BSA association on BSA-dye reagent interactions. UV-visible spectroscopic analysis of the FA-BSA mixture showed that the absorbance maximum of BSA-dye reagent occurred at 595nm, even after the association of FA with BSA. This confirms that protonated amino acid groups of the protein are not involved in FA-BSA association. Moreover, molecular dynamics (MD) simulation confirmed the presence of an associative interaction between aromatic moieties in FA and tryptophan moieties in the indolicidin molecule, which disrupted FA self-assembly. An X-ray diffraction (XRD) study showed that there was limited disruption of FA self-assembly after the addition of BSA or tryptophan. This suggests that FA and BSA are compatible and associate with each other. Graphical Abstract Mechanism of folic acid and protein association | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a Folate |2 nationallicence | |
| 690 | 7 | |a Tryptophan |2 nationallicence | |
| 690 | 7 | |a Molecular dynamics |2 nationallicence | |
| 690 | 7 | |a Indolicidin |2 nationallicence | |
| 690 | 7 | |a Spectroscopy |2 nationallicence | |
| 700 | 1 | |a Gupta |D Rajat |u Department of Chemical Engineering, Indian Institute of Technology Delhi, 110016, New Delhi, India |4 aut | |
| 700 | 1 | |a Kalita |D Prasanta |u Department of Chemical Engineering, Indian Institute of Technology Delhi, 110016, New Delhi, India |4 aut | |
| 700 | 1 | |a Patil |D Omkar |u Department of Chemical Engineering, Indian Institute of Technology Delhi, 110016, New Delhi, India |4 aut | |
| 700 | 1 | |a Mohanty |D Sanat |u Department of Chemical Engineering, Indian Institute of Technology Delhi, 110016, New Delhi, India |4 aut | |
| 773 | 0 | |t Journal of Molecular Modeling |d Springer Berlin Heidelberg |g 21/12(2015-12-01), 1-8 |x 1610-2940 |q 21:12<1 |1 2015 |2 21 |o 894 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00894-015-2847-2 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00894-015-2847-2 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Gupta |D Rajat |u Department of Chemical Engineering, Indian Institute of Technology Delhi, 110016, New Delhi, India |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Kalita |D Prasanta |u Department of Chemical Engineering, Indian Institute of Technology Delhi, 110016, New Delhi, India |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Patil |D Omkar |u Department of Chemical Engineering, Indian Institute of Technology Delhi, 110016, New Delhi, India |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Mohanty |D Sanat |u Department of Chemical Engineering, Indian Institute of Technology Delhi, 110016, New Delhi, India |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Journal of Molecular Modeling |d Springer Berlin Heidelberg |g 21/12(2015-12-01), 1-8 |x 1610-2940 |q 21:12<1 |1 2015 |2 21 |o 894 | ||