caa a22 4500 605513872 CHVBK 20210128100702.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s00360-014-0871-7 doi (NATIONALLICENCE)springer-10.1007/s00360-014-0871-7 Acclimation to different environmental salinities induces molecular endocrine changes in the GH/IGF-I axis of juvenile gilthead sea bream ( Sparus aurata L.) [Elektronische Daten] [Khaled Mohammed-Geba, J. Mancera, G. Martínez-Rodríguez] To assess the role of the GH/IGF-I axis in osmotic acclimation of the gilthead seabream Sparus aurata, juvenile specimens were acclimated to four environmental salinities: hyposmotic (5‰), isosmotic (12‰) and hyperosmotic (40 and 55‰). The full-length cDNAs for both pituitary adenylate cyclase-activating peptide (PACAP) and prepro-somatostatin-I (PSS-I), the precursor for mature somatostatin-I (SS-I), were cloned. Hypothalamic PACAP and PSS-I, hypophyseal growth hormone (GH) and prolactin (PRL), and hepatic insulin-like growth factor-I (IGF-I) mRNA expression levels were analyzed in the four rearing salinities tested. PACAP and IGF-I mRNA values increased significantly in response to both 5 and 55‰ salinities, showing a U-shaped curve relationship with the basal level in the 40‰ group. Hypothalamic PSS-I expression increased strongly in the 55‰ environment. GH mRNA levels did not change in any of the tested environmental salinities. PRL mRNA maximum levels were encountered in the 5 and 12‰ environments, but significantly down-regulated in the 40‰. Plasma cortisol levels significantly increased in the 40‰ environment. These results are discussed in relation to the well-known high adaptability of Sparus aurata to different environmental salinities and the role of the GH/IGF-I axis in this process. Springer-Verlag Berlin Heidelberg, 2014 GH nationallicence IGF-I nationallicence Osmoregulation nationallicence PACAP nationallicence PSS-I nationallicence Sparus aurata nationallicence Mohammed-Geba Khaled Departamento de Biología Marina y Acuicultura, Instituto de Ciencias Marinas de Andalucía, Consejo Superior Investigaciones Científicas (CSIC), 11510, Puerto Real, Cádiz, Spain aut Mancera J. Departamento de Biología, Facultad de Ciencias del Mar y Ambientales, Campus de Excelencia Internacional del Mar (CEIMAR), Universidad de Cádiz, 11510, Puerto Real, Cádiz, Spain aut Martínez-Rodríguez G. Departamento de Biología Marina y Acuicultura, Instituto de Ciencias Marinas de Andalucía, Consejo Superior Investigaciones Científicas (CSIC), 11510, Puerto Real, Cádiz, Spain aut Journal of Comparative Physiology B Springer Berlin Heidelberg 185/1(2015-01-01), 87-101 0174-1578 185:1<87 2015 185 360 https://doi.org/10.1007/s00360-014-0871-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00360-014-0871-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mohammed-Geba Khaled Departamento de Biología Marina y Acuicultura, Instituto de Ciencias Marinas de Andalucía, Consejo Superior Investigaciones Científicas (CSIC), 11510, Puerto Real, Cádiz, Spain aut NATIONALLICENCE 700 1- Mancera J. Departamento de Biología, Facultad de Ciencias del Mar y Ambientales, Campus de Excelencia Internacional del Mar (CEIMAR), Universidad de Cádiz, 11510, Puerto Real, Cádiz, Spain aut NATIONALLICENCE 700 1- Martínez-Rodríguez G. Departamento de Biología Marina y Acuicultura, Instituto de Ciencias Marinas de Andalucía, Consejo Superior Investigaciones Científicas (CSIC), 11510, Puerto Real, Cádiz, Spain aut NATIONALLICENCE 773 0- Journal of Comparative Physiology B Springer Berlin Heidelberg 185/1(2015-01-01), 87-101 0174-1578 185:1<87 2015 185 360