caa a22 4500 605513929 CHVBK 20210128100702.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s00360-014-0870-8 doi (NATIONALLICENCE)springer-10.1007/s00360-014-0870-8 Dietary fatty acids affect mitochondrial phospholipid compositions and mitochondrial gene expression of rainbow trout liver at different ages [Elektronische Daten] [P. Almaida-Pagán, C. De Santis, O. Rubio-Mejía, D. Tocher] Mitochondria are among the first responders to various stressors that challenge the homeostasis of cells and organisms. Mitochondrial decay is generally associated with impairment in the organelle bioenergetics function and increased oxidative stress, and it appears that deterioration of mitochondrial inner membrane phospholipids (PL), particularly cardiolipin (CL), and accumulation of mitochondrial DNA (mtDNA) mutations are among the main mechanisms involved in this process. In the present study, liver mitochondrial membrane PL compositions, lipid peroxidation, and mtDNA gene expression were analyzed in rainbow trout fed three diets with the same base formulation but with lipid supplied either by fish oil (FO), rapeseed oil (RO), or high DHA oil (DHA) during 6weeks. Specifically, two feeding trials were performed using fish from the same population of two ages (1 and 3years), and PL class compositions of liver mitochondria, fatty acid composition of individual PL classes, TBARS content, and mtDNA expression were determined. Dietary fatty acid composition strongly affected mitochondrial membrane composition from trout liver but observed changes did not fully reflect the diet, particularly when it contained high DHA. The changes were PL specific, CL being particularly resistant to changes in DHA. Some significant differences observed in expression of mtDNA with diet may suggest long-term dietary effects in mitochondrial gene expression which could affect electron transport chain function. All the changes were influenced by fish age, which could be related to the different growth rates observed between 1- and 3-year-old trout but that could also indicate age-related changes in the ability to maintain structural homeostasis of mitochondrial membranes. Springer-Verlag Berlin Heidelberg, 2014 Cardiolipin nationallicence Diet nationallicence Fatty acid nationallicence Mitochondria nationallicence Rainbow trout nationallicence Oxidative stress nationallicence Phospholipid nationallicence ANT : Nucleotide translocase nationallicence BHT : Butylated hydroxytoluene nationallicence cDNA : Complementary DNA nationallicence CL : Cardiolipin nationallicence COX : Cytochrome c oxidase complex nationallicence DHA : Docosahexaenoic acid nationallicence E : PCR efficiency nationallicence EPA : Eicosapentaenoic acid nationallicence ETC : Electron transport chain nationallicence FA : Fatty acid nationallicence FAME : Fatty acid methyl esters nationallicence HPTLC : High performance thin layer chromatography nationallicence HUFA : Highly unsaturated fatty acids nationallicence LA : Linoleic acid nationallicence LC-PUFA : Long-chain polyunsaturated fatty acid nationallicence MPH : Membrane pacemaker hypothesis nationallicence mtDNA : Mitochondrial DNA nationallicence MUFA : Monounsaturated fatty acids nationallicence NAC : No-amplification control nationallicence ND : NADH-coenzyme Q oxidoreductase complex nationallicence NTC : No-template control nationallicence PC : Phosphatidylcholine nationallicence PE : Phosphatidylethanolamine nationallicence PI : Phosphatidylinositol nationallicence PIn : Peroxidation index nationallicence PL : Phospholipid nationallicence PS : Phosphatidylserine nationallicence PUFA : Polyunsaturated fatty acid nationallicence RO : Rapeseed oil nationallicence ROS : Reactive oxygen species nationallicence SFA : Saturated fatty acids nationallicence FO : Fish oil nationallicence SM : Sphingomyelin nationallicence RT-PCR : Real-time PCR nationallicence TBARS : Thiobarbituric acid reactive substances nationallicence TBA : Thiobarbituric acid nationallicence TCA : Trichloroacetic acid nationallicence TLC : Thin layer chromatography nationallicence Almaida-Pagán P. School of Natural Sciences, Institute of Aquaculture, University of Stirling, FK9 4LA, Stirling, Scotland, UK aut De Santis C. School of Natural Sciences, Institute of Aquaculture, University of Stirling, FK9 4LA, Stirling, Scotland, UK aut Rubio-Mejía O. School of Natural Sciences, Institute of Aquaculture, University of Stirling, FK9 4LA, Stirling, Scotland, UK aut Tocher D. School of Natural Sciences, Institute of Aquaculture, University of Stirling, FK9 4LA, Stirling, Scotland, UK aut Journal of Comparative Physiology B Springer Berlin Heidelberg 185/1(2015-01-01), 73-86 0174-1578 185:1<73 2015 185 360 https://doi.org/10.1007/s00360-014-0870-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00360-014-0870-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Almaida-Pagán P. School of Natural Sciences, Institute of Aquaculture, University of Stirling, FK9 4LA, Stirling, Scotland, UK aut NATIONALLICENCE 700 1- De Santis C. School of Natural Sciences, Institute of Aquaculture, University of Stirling, FK9 4LA, Stirling, Scotland, UK aut NATIONALLICENCE 700 1- Rubio-Mejía O. School of Natural Sciences, Institute of Aquaculture, University of Stirling, FK9 4LA, Stirling, Scotland, UK aut NATIONALLICENCE 700 1- Tocher D. School of Natural Sciences, Institute of Aquaculture, University of Stirling, FK9 4LA, Stirling, Scotland, UK aut NATIONALLICENCE 773 0- Journal of Comparative Physiology B Springer Berlin Heidelberg 185/1(2015-01-01), 73-86 0174-1578 185:1<73 2015 185 360