caa a22 4500 605513937 CHVBK 20210128100702.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s00360-014-0864-6 doi (NATIONALLICENCE)springer-10.1007/s00360-014-0864-6 Parental environmental exposure leads to glycometabolic disturbances that affect fertilization of eggs in the silkworm Bombyx mori [Elektronische Daten] the parental transcript legacy [H. Tao, H. Liu, Y. Cheng, Y. Sima, W. Yin, S. Xu] Parental transcript legacy plays an important role in fertilization and development of the early embryo. Parental environmental exposure affects the fertilization of eggs, but the underlying biochemical mechanism is largely unresolved. In this study, the parental environmental effects on fertilization of eggs were explored in the silkworm Bombyx mori (B. mori), an ideal lepidopteran animal model. The results showed that the rate of fertilization decreased after the parents were exposed to a poor environment at 32°C with continuous illumination for 72h on days 6-9 of the pupal stage, which is a key period for germ cell maturation. This was likely attributable to lower energy charge values, obstructed nicotinamide adenine dinucleotide (NAD+) regeneration and inactive tricarboxylic acid cycle (TCA), leading to accumulation of large amounts of pyruvic acid and lactic acid. This effect was related to energy metabolism via glycolysis; in particular disruption of pyruvate metabolism. In conclusion, this study showed parental exposure to an abnormal environment during germ cell maturation affected glycolysis and the subsequent fertilization of eggs via the parental transcript legacy in B. mori. Springer-Verlag Berlin Heidelberg, 2014 Bombyx mori nationallicence Fertilization nationallicence Glycolysis nationallicence Environmental exposure nationallicence Parental transcript legacy nationallicence 25LD : 12-hours light-dark cycles at 25°C nationallicence 32LL : Constant illumination at 32°C nationallicence DEM : Disorders of energy metabolism nationallicence E0 : Eggs from virgin females nationallicence E1-E3 : Eggs collected at 1-3h after oviposition nationallicence Tao H. Department of Applied Biology, School of Biology and Basic Medical Sciences, Soochow University, 215123, Suzhou, China aut Liu H. Department of Applied Biology, School of Biology and Basic Medical Sciences, Soochow University, 215123, Suzhou, China aut Cheng Y. Department of Applied Biology, School of Biology and Basic Medical Sciences, Soochow University, 215123, Suzhou, China aut Sima Y. Department of Applied Biology, School of Biology and Basic Medical Sciences, Soochow University, 215123, Suzhou, China aut Yin W. Institute for Cardiovascular Science, Soochow University, 215123, Suzhou, China aut Xu S. Department of Applied Biology, School of Biology and Basic Medical Sciences, Soochow University, 215123, Suzhou, China aut Journal of Comparative Physiology B Springer Berlin Heidelberg 185/1(2015-01-01), 47-55 0174-1578 185:1<47 2015 185 360 https://doi.org/10.1007/s00360-014-0864-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00360-014-0864-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tao H. Department of Applied Biology, School of Biology and Basic Medical Sciences, Soochow University, 215123, Suzhou, China aut NATIONALLICENCE 700 1- Liu H. Department of Applied Biology, School of Biology and Basic Medical Sciences, Soochow University, 215123, Suzhou, China aut NATIONALLICENCE 700 1- Cheng Y. Department of Applied Biology, School of Biology and Basic Medical Sciences, Soochow University, 215123, Suzhou, China aut NATIONALLICENCE 700 1- Sima Y. Department of Applied Biology, School of Biology and Basic Medical Sciences, Soochow University, 215123, Suzhou, China aut NATIONALLICENCE 700 1- Yin W. Institute for Cardiovascular Science, Soochow University, 215123, Suzhou, China aut NATIONALLICENCE 700 1- Xu S. Department of Applied Biology, School of Biology and Basic Medical Sciences, Soochow University, 215123, Suzhou, China aut NATIONALLICENCE 773 0- Journal of Comparative Physiology B Springer Berlin Heidelberg 185/1(2015-01-01), 47-55 0174-1578 185:1<47 2015 185 360