caa a22 4500 605514054 CHVBK 20210128100703.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s00360-015-0905-9 doi (NATIONALLICENCE)springer-10.1007/s00360-015-0905-9 Proteomics approaches shed new light on hibernation physiology [Elektronische Daten] [Katharine Grabek, Sandra Martin, Allyson Hindle] The broad phylogenetic distribution and rapid phenotypic transitions of mammalian hibernators imply that hibernation is accomplished by differential expression of common genes. Traditional candidate gene approaches have thus far explained little of the molecular mechanisms underlying hibernation, likely due to (1) incomplete and imprecise sampling of a complex phenotype, and (2) the forming of hypotheses about which genes might be important based on studies of model organisms incapable of such dynamic physiology. Unbiased screening approaches, such as proteomics, offer an alternative means to discover the cellular underpinnings that permit successful hibernation and may reveal previously overlooked, important pathways. Here, we review the findings that have emerged from proteomics studies of hibernation. One striking feature is the stability of the proteome, especially across the extreme physiological shifts of torpor-arousal cycles during hibernation. This has led to subsequent investigations of the role of post-translational protein modifications in altering protein activity without energetically wasteful removal and rebuilding of protein pools. Another unexpected finding is the paucity of universal proteomic adjustments across organ systems in response to the extreme metabolic fluctuations despite the universality of their physiological challenges; rather each organ appears to respond in a unique, tissue-specific manner. Additional research is needed to extend and synthesize these results before it will be possible to address the whole body physiology of hibernation. Springer-Verlag Berlin Heidelberg, 2015 Ground squirrel nationallicence Bear nationallicence Bat nationallicence Metabolic depression nationallicence Grabek Katharine Department of Cell and Developmental Biology, University of Colorado School of Medicine, 80045, Aurora, CO, USA aut Martin Sandra Department of Cell and Developmental Biology, University of Colorado School of Medicine, 80045, Aurora, CO, USA aut Hindle Allyson Department of Cell and Developmental Biology, University of Colorado School of Medicine, 80045, Aurora, CO, USA aut Journal of Comparative Physiology B Springer Berlin Heidelberg 185/6(2015-08-01), 607-627 0174-1578 185:6<607 2015 185 360 https://doi.org/10.1007/s00360-015-0905-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 review-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00360-015-0905-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Grabek Katharine Department of Cell and Developmental Biology, University of Colorado School of Medicine, 80045, Aurora, CO, USA aut NATIONALLICENCE 700 1- Martin Sandra Department of Cell and Developmental Biology, University of Colorado School of Medicine, 80045, Aurora, CO, USA aut NATIONALLICENCE 700 1- Hindle Allyson Department of Cell and Developmental Biology, University of Colorado School of Medicine, 80045, Aurora, CO, USA aut NATIONALLICENCE 773 0- Journal of Comparative Physiology B Springer Berlin Heidelberg 185/6(2015-08-01), 607-627 0174-1578 185:6<607 2015 185 360