caa a22 4500 605514062 CHVBK 20210128100703.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s00360-015-0913-9 doi (NATIONALLICENCE)springer-10.1007/s00360-015-0913-9 Comparisons of two types of teleostean pseudobranchs, silver moony ( Monodactylus argenteus ) and tilapia ( Oreochromis mossambicus ), with salinity-dependent morphology and ion transporter expression [Elektronische Daten] [Sheng-Hui Yang, Chao-Kai Kang, Yau-Chung Hu, Ling-Chia Yen, Shu-Chuan Tsai, Yueh-Ling Hsieh, Tsung-Han Lee] There are essentially four different morphological types of pseudobranchs in teleosts, including lamellae-free, lamellae semi-free, covered, and embedded types. In the euryhaline silver moony (Monodactylus argenteus), the pseudobranch belongs to the lamellae semi-free type, which is characterized by one row of filaments on the opercular membrane and fusion on the buccal edge. The pseudobranchial epithelium of the moony contains two types of Na+, K+-ATPase (NKA)-rich cells: chloride cells (CCs) and pseudobranch-type cells (PSCs). Our results revealed increased expression of NKA, the Na+, K+, 2Cl− cotransporter (NKCC), and the cystic fibrosis transmembrane conductance regulator (CFTR) for Cl− secretion and CCs profiles in the pseudobranchs of seawater (SW)-acclimated silver moonies, which indicates the potential role of pseudobranchs containing CCs in hypo-osmoregulation. In contrast, the pseudobranch of the Mozambique tilapia (Oreochromis mossambicus) belongs to the embedded type, which is covered by the connective tissues and only contains PSCs but not CCs. No sign of NKCC and CFTR-immunoreactivity (IR) was found in the pseudobranchs of SW and freshwater (FW) tilapia. However, higher NKA protein expression and larger sizes of NKA-IR PSCs were found in the pseudobranchs of FW-acclimated tilapia. Moreover, in the FW-acclimated moony, NKA-IR PSCs also exhibited higher numbers and larger sizes than in the SW individuals. Taken together, similar responses in low-salinity environments in different types of pseudobranchs indicated that the salinity-dependent morphologies of PSCs might be involved in critical functions for FW teleosts. Springer-Verlag Berlin Heidelberg, 2015 Pseudobranch nationallicence Osmoregulation nationallicence Na+, K+-ATPase nationallicence Na+, K+, 2Cl− cotransporter nationallicence Silver moony nationallicence Tilapia nationallicence Yang Sheng-Hui Department of Life Sciences, National Chung Hsing University, 250, Kuo-Kuang Road, 402, Taichung, Taiwan aut Kang Chao-Kai Tainan Hydraulics Laboratory, National Cheng Kung University, 709, Tainan, Taiwan aut Hu Yau-Chung Program in Microbial Genomics, National Chung Hsing University, 402, Taichung, Taiwan aut Yen Ling-Chia Department of Life Sciences, National Chung Hsing University, 250, Kuo-Kuang Road, 402, Taichung, Taiwan aut Tsai Shu-Chuan Institute of Life Sciences, Central Taiwan University of Science and Technology, 406, Taichung, Taiwan aut Hsieh Yueh-Ling Department of Physical Therapy, China Medical University, 404, Taichung, Taiwan aut Lee Tsung-Han Department of Life Sciences, National Chung Hsing University, 250, Kuo-Kuang Road, 402, Taichung, Taiwan aut Journal of Comparative Physiology B Springer Berlin Heidelberg 185/6(2015-08-01), 677-693 0174-1578 185:6<677 2015 185 360 https://doi.org/10.1007/s00360-015-0913-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00360-015-0913-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yang Sheng-Hui Department of Life Sciences, National Chung Hsing University, 250, Kuo-Kuang Road, 402, Taichung, Taiwan aut NATIONALLICENCE 700 1- Kang Chao-Kai Tainan Hydraulics Laboratory, National Cheng Kung University, 709, Tainan, Taiwan aut NATIONALLICENCE 700 1- Hu Yau-Chung Program in Microbial Genomics, National Chung Hsing University, 402, Taichung, Taiwan aut NATIONALLICENCE 700 1- Yen Ling-Chia Department of Life Sciences, National Chung Hsing University, 250, Kuo-Kuang Road, 402, Taichung, Taiwan aut NATIONALLICENCE 700 1- Tsai Shu-Chuan Institute of Life Sciences, Central Taiwan University of Science and Technology, 406, Taichung, Taiwan aut NATIONALLICENCE 700 1- Hsieh Yueh-Ling Department of Physical Therapy, China Medical University, 404, Taichung, Taiwan aut NATIONALLICENCE 700 1- Lee Tsung-Han Department of Life Sciences, National Chung Hsing University, 250, Kuo-Kuang Road, 402, Taichung, Taiwan aut NATIONALLICENCE 773 0- Journal of Comparative Physiology B Springer Berlin Heidelberg 185/6(2015-08-01), 677-693 0174-1578 185:6<677 2015 185 360