Calcium response of KCl-excited populations of ventricular myocytes from the European sea bass ( Dicentrarchus labrax ): a promising approach to integrate cell-to-cell heterogeneity in studying the cellular basis of fish cardiac performance
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| 024 | 7 | 0 | |a 10.1007/s00360-015-0924-6 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00360-015-0924-6 | ||
| 245 | 0 | 0 | |a Calcium response of KCl-excited populations of ventricular myocytes from the European sea bass ( Dicentrarchus labrax ): a promising approach to integrate cell-to-cell heterogeneity in studying the cellular basis of fish cardiac performance |h [Elektronische Daten] |c [Hélène Ollivier, James Marchant, Nicolas Le Bayon, Arianna Servili, Guy Claireaux] |
| 520 | 3 | |a Climate change challenges the capacity of fishes to thrive in their habitat. However, through phenotypic diversity, they demonstrate remarkable resilience to deteriorating conditions. In fish populations, inter-individual variation in a number of fitness-determining physiological traits, including cardiac performance, is classically observed. Information about the cellular bases of inter-individual variability in cardiac performance is scarce including the possible contribution of excitation-contraction (EC) coupling. This study aimed at providing insight into EC coupling-related Ca2+ response and thermal plasticity in the European sea bass (Dicentrarchus labrax). A cell population approach was used to lay the methodological basis for identifying the cellular determinants of cardiac performance. Fish were acclimated at 12 and 22°C and changes in intracellular calcium concentration ([Ca2+]i) following KCl stimulation were measured using Fura-2, at 12 or 22°C-test. The increase in [Ca2+]i resulted primarily from extracellular Ca2+ entry but sarcoplasmic reticulum stores were also shown to be involved. As previously reported in sea bass, a modest effect of adrenaline was observed. Moreover, although the response appeared relatively insensitive to an acute temperature change, a difference in Ca2+ response was observed between 12- and 22°C-acclimated fish. In particular, a greater increase in [Ca2+]i at a high level of adrenaline was observed in 22°C-acclimated fish that may be related to an improved efficiency of adrenaline under these conditions. In conclusion, this method allows a rapid screening of cellular characteristics. It represents a promising tool to identify the cellular determinants of inter-individual variability in fishes' capacity for environmental adaptation. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a Sea bass |2 nationallicence | |
| 690 | 7 | |a Myocyte populations |2 nationallicence | |
| 690 | 7 | |a Calcium signaling |2 nationallicence | |
| 690 | 7 | |a Cardiac plasticity |2 nationallicence | |
| 690 | 7 | |a Inter-individual variability |2 nationallicence | |
| 700 | 1 | |a Ollivier |D Hélène |u ORPHY, Université de Bretagne Occidentale, Brest, France |4 aut | |
| 700 | 1 | |a Marchant |D James |u Unité PFOM-ARN, LEMAR, Centre Ifremer de Brest, Plouzané, France |4 aut | |
| 700 | 1 | |a Le Bayon |D Nicolas |u Unité PFOM-ARN, LEMAR, Centre Ifremer de Brest, Plouzané, France |4 aut | |
| 700 | 1 | |a Servili |D Arianna |u Unité PFOM-ARN, LEMAR, Centre Ifremer de Brest, Plouzané, France |4 aut | |
| 700 | 1 | |a Claireaux |D Guy |u Unité PFOM-ARN, LEMAR, Centre Ifremer de Brest, Plouzané, France |4 aut | |
| 773 | 0 | |t Journal of Comparative Physiology B |d Springer Berlin Heidelberg |g 185/7(2015-10-01), 755-765 |x 0174-1578 |q 185:7<755 |1 2015 |2 185 |o 360 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00360-015-0924-6 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00360-015-0924-6 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Ollivier |D Hélène |u ORPHY, Université de Bretagne Occidentale, Brest, France |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Marchant |D James |u Unité PFOM-ARN, LEMAR, Centre Ifremer de Brest, Plouzané, France |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Le Bayon |D Nicolas |u Unité PFOM-ARN, LEMAR, Centre Ifremer de Brest, Plouzané, France |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Servili |D Arianna |u Unité PFOM-ARN, LEMAR, Centre Ifremer de Brest, Plouzané, France |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Claireaux |D Guy |u Unité PFOM-ARN, LEMAR, Centre Ifremer de Brest, Plouzané, France |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Journal of Comparative Physiology B |d Springer Berlin Heidelberg |g 185/7(2015-10-01), 755-765 |x 0174-1578 |q 185:7<755 |1 2015 |2 185 |o 360 | ||